Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2117 and GFF2118 overlap by 4 nucleotides GFF2118 and GFF2119 overlap by 4 nucleotides GFF2119 and GFF2120 overlap by 4 nucleotides GFF2120 and GFF2121 are separated by 25 nucleotides
GFF2117 - KEGG: type IV fimbrial biogenesis protein FimT, at 167,355 to 167,906
GFF2117
GFF2118 - hypothetical protein, at 167,903 to 168,460
GFF2118
GFF2119 - Type IV fimbrial biogenesis protein PilW, at 168,457 to 169,221
GFF2119
GFF2120 - Type IV fimbrial biogenesis protein PilX, at 169,218 to 169,799
GFF2120
GFF2121 - Type IV fimbrial biogenesis protein PilY1, at 169,825 to 173,256
GFF2121
Position (kb) 168
169
170 Strain fitness (log2 ratio) -1
0
1
2 at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.497 kb on - strand, within GFF2117 at 167.517 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 168.028 kb on + strand, within GFF2118 at 168.245 kb on + strand, within GFF2118 at 168.290 kb on - strand, within GFF2118 at 168.293 kb on + strand, within GFF2118 at 168.371 kb on + strand, within GFF2118 at 168.372 kb on - strand, within GFF2118 at 168.447 kb on - strand at 168.829 kb on + strand, within GFF2119 at 168.829 kb on + strand, within GFF2119 at 168.915 kb on + strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.235 kb on + strand at 169.338 kb on + strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.507 kb on + strand, within GFF2120 at 169.563 kb on + strand, within GFF2120 at 169.629 kb on + strand, within GFF2120 at 169.630 kb on - strand, within GFF2120 at 169.633 kb on - strand, within GFF2120 at 169.660 kb on - strand, within GFF2120 at 169.758 kb on + strand at 169.759 kb on - strand at 169.942 kb on + strand at 169.942 kb on + strand at 169.943 kb on - strand at 170.029 kb on + strand at 170.029 kb on + strand at 170.029 kb on + strand at 170.030 kb on - strand at 170.033 kb on - strand at 170.033 kb on - strand at 170.033 kb on - strand at 170.126 kb on + strand at 170.198 kb on + strand, within GFF2121
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 167,464 + GFF2117 0.20 +0.2 167,464 + GFF2117 0.20 +1.4 167,464 + GFF2117 0.20 +0.1 167,497 - GFF2117 0.26 +0.5 167,517 + GFF2117 0.29 +0.5 167,742 + GFF2117 0.70 +0.1 167,742 + GFF2117 0.70 -0.1 167,743 - GFF2117 0.70 -0.5 167,743 - GFF2117 0.70 +1.0 167,743 - GFF2117 0.70 +0.9 167,743 - GFF2117 0.70 +0.4 168,028 + GFF2118 0.22 -0.2 168,245 + GFF2118 0.61 +1.0 168,290 - GFF2118 0.69 +0.3 168,293 + GFF2118 0.70 +0.5 168,371 + GFF2118 0.84 -0.9 168,372 - GFF2118 0.84 -0.6 168,447 - -1.4 168,829 + GFF2119 0.49 -0.0 168,829 + GFF2119 0.49 +0.8 168,915 + GFF2119 0.60 -1.4 168,916 - GFF2119 0.60 -0.2 168,916 - GFF2119 0.60 +0.2 168,916 - GFF2119 0.60 -0.4 169,027 + GFF2119 0.75 -0.2 169,027 + GFF2119 0.75 -0.2 169,027 + GFF2119 0.75 -1.7 169,028 - GFF2119 0.75 +0.1 169,028 - GFF2119 0.75 +1.0 169,028 - GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 -0.8 169,028 - GFF2119 0.75 -1.0 169,028 - GFF2119 0.75 +0.2 169,028 - GFF2119 0.75 -0.3 169,235 + +0.2 169,338 + GFF2120 0.21 -1.7 169,339 - GFF2120 0.21 -0.5 169,339 - GFF2120 0.21 -0.1 169,339 - GFF2120 0.21 -1.2 169,339 - GFF2120 0.21 +0.8 169,507 + GFF2120 0.50 +0.2 169,563 + GFF2120 0.59 +1.5 169,629 + GFF2120 0.71 -0.2 169,630 - GFF2120 0.71 +0.2 169,633 - GFF2120 0.71 -0.5 169,660 - GFF2120 0.76 -0.4 169,758 + +0.1 169,759 - +0.4 169,942 + -0.1 169,942 + -0.7 169,943 - -0.5 170,029 + -0.7 170,029 + -0.4 170,029 + -0.5 170,030 - -0.5 170,033 - -0.0 170,033 - +0.6 170,033 - +0.3 170,126 + +2.0 170,198 + GFF2121 0.11 -0.7
Or see this region's nucleotide sequence