Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2083 and GFF2084 overlap by 4 nucleotides GFF2084 and GFF2085 overlap by 4 nucleotides GFF2085 and GFF2086 overlap by 4 nucleotides GFF2086 and GFF2087 overlap by 11 nucleotides
GFF2083 - putative periplasmic membrane protein, at 131,361 to 131,963
GFF2083
GFF2084 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 131,960 to 132,913
GFF2084
GFF2085 - Acyl-CoA:1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 132,910 to 133,638
GFF2085
GFF2086 - hypothetical protein, at 133,635 to 134,084
GFF2086
GFF2087 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 134,074 to 135,423
GFF2087
Position (kb) 132
133
134 Strain fitness (log2 ratio) -2
-1
0
1
2 at 131.976 kb on + strand at 131.994 kb on + strand at 131.994 kb on + strand at 131.995 kb on - strand at 132.186 kb on + strand, within GFF2084 at 132.187 kb on - strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.522 kb on + strand, within GFF2084 at 132.522 kb on + strand, within GFF2084 at 132.523 kb on - strand, within GFF2084 at 132.523 kb on - strand, within GFF2084 at 132.534 kb on + strand, within GFF2084 at 132.534 kb on + strand, within GFF2084 at 132.534 kb on + strand, within GFF2084 at 132.535 kb on - strand, within GFF2084 at 132.535 kb on - strand, within GFF2084 at 132.535 kb on - strand, within GFF2084 at 132.535 kb on - strand, within GFF2084 at 132.535 kb on - strand, within GFF2084 at 132.544 kb on - strand, within GFF2084 at 132.544 kb on - strand, within GFF2084 at 132.576 kb on + strand, within GFF2084 at 132.576 kb on + strand, within GFF2084 at 132.576 kb on + strand, within GFF2084 at 132.576 kb on + strand, within GFF2084 at 132.577 kb on - strand, within GFF2084 at 132.577 kb on - strand, within GFF2084 at 132.577 kb on - strand, within GFF2084 at 132.577 kb on - strand, within GFF2084 at 132.577 kb on - strand, within GFF2084 at 132.597 kb on + strand, within GFF2084 at 132.598 kb on - strand, within GFF2084 at 132.639 kb on + strand, within GFF2084 at 132.640 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.467 kb on - strand, within GFF2085 at 133.891 kb on + strand, within GFF2086 at 133.891 kb on + strand, within GFF2086 at 133.892 kb on - strand, within GFF2086
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 131,976 + -0.9 131,994 + -1.4 131,994 + -0.4 131,995 - -0.5 132,186 + GFF2084 0.24 +2.0 132,187 - GFF2084 0.24 -0.8 132,303 + GFF2084 0.36 -0.7 132,303 + GFF2084 0.36 +0.8 132,303 + GFF2084 0.36 +1.2 132,303 + GFF2084 0.36 +0.8 132,304 - GFF2084 0.36 -1.3 132,304 - GFF2084 0.36 +0.2 132,304 - GFF2084 0.36 -0.5 132,304 - GFF2084 0.36 -0.5 132,304 - GFF2084 0.36 +0.2 132,522 + GFF2084 0.59 +0.5 132,522 + GFF2084 0.59 -1.2 132,523 - GFF2084 0.59 +0.8 132,523 - GFF2084 0.59 -0.3 132,534 + GFF2084 0.60 -0.3 132,534 + GFF2084 0.60 +0.8 132,534 + GFF2084 0.60 -0.4 132,535 - GFF2084 0.60 -0.7 132,535 - GFF2084 0.60 -0.7 132,535 - GFF2084 0.60 -0.4 132,535 - GFF2084 0.60 -0.7 132,535 - GFF2084 0.60 -0.4 132,544 - GFF2084 0.61 -0.9 132,544 - GFF2084 0.61 +0.8 132,576 + GFF2084 0.65 -0.3 132,576 + GFF2084 0.65 -0.6 132,576 + GFF2084 0.65 +0.9 132,576 + GFF2084 0.65 -1.1 132,577 - GFF2084 0.65 -0.4 132,577 - GFF2084 0.65 -1.0 132,577 - GFF2084 0.65 -1.2 132,577 - GFF2084 0.65 -0.5 132,577 - GFF2084 0.65 -0.3 132,597 + GFF2084 0.67 -1.8 132,598 - GFF2084 0.67 -1.0 132,639 + GFF2084 0.71 -0.0 132,640 - GFF2084 0.71 -0.4 132,670 - GFF2084 0.74 -0.2 132,670 - GFF2084 0.74 +0.9 132,670 - GFF2084 0.74 -1.2 133,367 + GFF2085 0.63 -0.7 133,367 + GFF2085 0.63 -2.2 133,367 + GFF2085 0.63 -0.7 133,466 + GFF2085 0.76 -1.5 133,466 + GFF2085 0.76 +0.6 133,467 - GFF2085 0.76 -0.3 133,891 + GFF2086 0.57 +0.6 133,891 + GFF2086 0.57 -0.4 133,892 - GFF2086 0.57 +0.2
Or see this region's nucleotide sequence