Strain Fitness in Variovorax sp. SCN45 around GFF2027

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
68,664	+		GFF2024	0.79	-0.4
68,664	+		GFF2024	0.79	+0.4
68,796	+				+0.8
68,796	+				-0.3
68,796	+				-0.1
68,797	-				-0.3
68,829	-				-0.0
69,023	+		GFF2025	0.43	-0.9
69,023	+		GFF2025	0.43	+0.0
69,023	+		GFF2025	0.43	-0.4
69,023	+		GFF2025	0.43	-0.3
69,024	-		GFF2025	0.44	-1.8
69,024	-		GFF2025	0.44	-0.8
69,024	-		GFF2025	0.44	-0.4
69,024	-		GFF2025	0.44	-0.2
69,024	-		GFF2025	0.44	-0.8
69,590	-				-1.4
69,640	+		GFF2027	0.14	-1.1
69,640	+		GFF2027	0.14	-0.6
69,640	+		GFF2027	0.14	-0.5
69,640	+		GFF2027	0.14	+0.0
69,640	+		GFF2027	0.14	+0.2
69,640	+		GFF2027	0.14	-1.9
69,641	-		GFF2027	0.14	-1.1
69,641	-		GFF2027	0.14	-0.3
69,641	-		GFF2027	0.14	-1.2
69,641	-		GFF2027	0.14	-0.8
69,641	-		GFF2027	0.14	-2.5
69,652	+		GFF2027	0.16	-2.2
69,652	+		GFF2027	0.16	-0.4
69,653	-		GFF2027	0.16	-0.9
69,653	-		GFF2027	0.16	+0.6
69,653	-		GFF2027	0.16	-0.4
69,736	+		GFF2027	0.29	-0.7
69,736	+		GFF2027	0.29	-2.0
69,736	+		GFF2027	0.29	-1.0
69,737	-		GFF2027	0.29	-0.7
69,737	-		GFF2027	0.29	+1.2
69,737	-		GFF2027	0.29	-0.2
69,802	+		GFF2027	0.39	-0.7
69,802	+		GFF2027	0.39	-2.5
69,802	+		GFF2027	0.39	-3.4
69,802	+		GFF2027	0.39	-1.1
69,802	+		GFF2027	0.39	-0.3
69,803	-		GFF2027	0.39	+0.4
69,803	-		GFF2027	0.39	-1.9
69,803	-		GFF2027	0.39	+0.6
69,803	-		GFF2027	0.39	-1.3
70,051	+		GFF2027	0.77	+0.1
70,051	+		GFF2027	0.77	-1.4
70,051	+		GFF2027	0.77	-1.4
70,051	+		GFF2027	0.77	-2.1
70,051	+		GFF2027	0.77	-2.0
70,051	+		GFF2027	0.77	-1.2
70,051	+		GFF2027	0.77	-1.0
70,051	+		GFF2027	0.77	-0.6
70,051	+		GFF2027	0.77	+0.6
70,052	-		GFF2027	0.77	-1.2
70,052	-		GFF2027	0.77	-1.4
70,052	-		GFF2027	0.77	-2.0
70,052	-		GFF2027	0.77	-2.1
70,052	-		GFF2027	0.77	+0.1
70,052	-		GFF2027	0.77	-1.1
70,108	+		GFF2027	0.86	-0.5
70,108	+		GFF2027	0.86	+2.9
70,108	-		GFF2027	0.86	-0.2
70,109	-		GFF2027	0.86	-0.7
70,260	+				-0.7
70,261	-				-0.4
70,261	-				-0.2
70,267	+				-0.7
70,360	+				-0.6
70,360	+				-1.0
70,360	+				-0.8
70,360	+				-0.5
70,360	+				-0.2
70,360	+				-1.7
70,360	+				-0.4
70,360	+				-0.0
70,360	+				+1.4
70,360	+				+0.7
70,361	-				-0.4
70,361	-				-0.4
70,361	-				-0.5
70,361	-				-2.5
70,361	-				-1.0
70,361	-				-1.0
70,361	-				+2.4
70,392	+				+0.3
70,392	+				-0.6
70,392	+				-2.3
70,392	+				-1.1
70,393	-				+0.8
70,393	-				-1.9
70,393	-				+1.2
70,491	-				+0.1
70,499	+				-0.9
70,499	+				-3.3
70,499	+				-0.8
70,499	+				+0.8
70,500	-				-1.3
70,500	-				+0.4
70,500	-				-0.4
70,500	-				+1.6
70,500	-				-0.4
70,505	+				-0.5
70,664	+		GFF2028	0.17	+1.2
70,664	+		GFF2028	0.17	-0.2
70,664	+		GFF2028	0.17	-1.5
70,664	+		GFF2028	0.17	-1.4
70,664	+		GFF2028	0.17	+2.4
70,664	+		GFF2028	0.17	-2.0
70,664	+		GFF2028	0.17	-1.2
70,664	+		GFF2028	0.17	-0.2
70,665	-		GFF2028	0.17	-0.4
70,665	-		GFF2028	0.17	-1.4
70,665	-		GFF2028	0.17	-1.8
70,665	-		GFF2028	0.17	-1.3
70,665	-		GFF2028	0.17	-0.4
70,665	-		GFF2028	0.17	-0.2
70,665	-		GFF2028	0.17	-0.7
70,665	-		GFF2028	0.17	-0.0
70,808	+		GFF2028	0.25	+1.2
70,826	+		GFF2028	0.26	-0.3
70,826	+		GFF2028	0.26	-0.6
70,826	+		GFF2028	0.26	+0.9
70,826	+		GFF2028	0.26	+0.5
70,826	+		GFF2028	0.26	-0.6
70,826	+		GFF2028	0.26	-0.8
70,826	+		GFF2028	0.26	+1.6
70,826	+		GFF2028	0.26	-0.7
70,826	+		GFF2028	0.26	+0.5
70,826	+		GFF2028	0.26	+0.2
70,826	+		GFF2028	0.26	-0.6
70,826	+		GFF2028	0.26	-0.5
70,826	+		GFF2028	0.26	-0.7
70,826	+		GFF2028	0.26	-1.2
70,826	+		GFF2028	0.26	-1.6
70,827	-		GFF2028	0.26	-0.0
70,827	-		GFF2028	0.26	-0.0
70,827	-		GFF2028	0.26	+0.2
70,827	-		GFF2028	0.26	-0.6
70,827	-		GFF2028	0.26	-0.3
70,827	-		GFF2028	0.26	-1.0
70,827	-		GFF2028	0.26	-0.8
70,827	-		GFF2028	0.26	-1.5
70,865	+		GFF2028	0.29	-0.1
70,865	+		GFF2028	0.29	-0.8
70,865	+		GFF2028	0.29	-2.2
70,865	+		GFF2028	0.29	-0.2
70,865	+		GFF2028	0.29	+0.2
70,866	-		GFF2028	0.29	-0.6
70,866	-		GFF2028	0.29	-0.9
70,866	-		GFF2028	0.29	+0.4
71,183	+		GFF2028	0.47	+0.0
71,184	-		GFF2028	0.47	+0.7
71,184	-		GFF2028	0.47	-0.8
71,184	-		GFF2028	0.47	+0.2