Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1816 and GFF1817 are separated by 74 nucleotides GFF1817 and GFF1818 are separated by 4 nucleotides GFF1818 and GFF1819 are separated by 56 nucleotides GFF1819 and GFF1820 are separated by 215 nucleotides
GFF1816 - Transcriptional regulator, GntR family, at 480,777 to 481,451
GFF1816
GFF1817 - 5-hydroxyisourate hydrolase (EC 3.5.2.17), at 481,526 to 481,876
GFF1817
GFF1818 - FIG00932412: hypothetical protein, at 481,881 to 482,378
GFF1818
GFF1819 - Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family, at 482,435 to 483,244
GFF1819
GFF1820 - FIG137887: membrane protein related to purine degradation, at 483,460 to 484,671
GFF1820
Position (kb) 482
483
484 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.476 kb on - strand at 481.545 kb on + strand at 481.546 kb on - strand at 481.595 kb on + strand, within GFF1817 at 481.595 kb on + strand, within GFF1817 at 481.596 kb on - strand, within GFF1817 at 481.596 kb on - strand, within GFF1817 at 481.673 kb on + strand, within GFF1817 at 481.673 kb on + strand, within GFF1817 at 481.703 kb on + strand, within GFF1817 at 481.704 kb on - strand, within GFF1817 at 481.730 kb on + strand, within GFF1817 at 481.730 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.862 kb on + strand at 481.863 kb on - strand at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.176 kb on + strand, within GFF1818 at 482.176 kb on + strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.363 kb on + strand at 482.363 kb on + strand at 482.364 kb on - strand at 482.388 kb on + strand at 482.388 kb on + strand at 482.424 kb on + strand at 482.720 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.383 kb on + strand at 483.383 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.469 kb on + strand at 483.470 kb on - strand at 483.470 kb on - strand at 483.470 kb on - strand at 483.470 kb on - strand at 483.472 kb on + strand at 483.472 kb on + strand at 483.472 kb on + strand at 483.473 kb on - strand at 483.547 kb on + strand at 483.548 kb on - strand at 483.649 kb on + strand, within GFF1820 at 483.649 kb on + strand, within GFF1820 at 483.649 kb on + strand, within GFF1820 at 483.650 kb on - strand, within GFF1820 at 483.650 kb on - strand, within GFF1820 at 483.650 kb on - strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.664 kb on + strand, within GFF1820 at 483.665 kb on - strand, within GFF1820 at 483.665 kb on - strand, within GFF1820 at 483.709 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.721 kb on + strand, within GFF1820 at 483.722 kb on - strand, within GFF1820 at 483.722 kb on - strand, within GFF1820 at 483.722 kb on - strand, within GFF1820 at 483.735 kb on + strand, within GFF1820 at 483.735 kb on + strand, within GFF1820 at 483.763 kb on + strand, within GFF1820 at 483.763 kb on + strand, within GFF1820 at 483.763 kb on + strand, within GFF1820 at 483.764 kb on - strand, within GFF1820 at 483.764 kb on - strand, within GFF1820 at 483.764 kb on - strand, within GFF1820 at 483.764 kb on - strand, within GFF1820 at 483.764 kb on - strand, within GFF1820 at 483.784 kb on + strand, within GFF1820 at 483.785 kb on - strand, within GFF1820 at 483.785 kb on - strand, within GFF1820 at 483.785 kb on - strand, within GFF1820 at 483.871 kb on + strand, within GFF1820 at 483.871 kb on + strand, within GFF1820 at 483.872 kb on - strand, within GFF1820 at 483.872 kb on - strand, within GFF1820 at 483.872 kb on - strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.156 kb on + strand, within GFF1820 at 484.157 kb on - strand, within GFF1820 at 484.157 kb on - strand, within GFF1820 at 484.157 kb on - strand, within GFF1820 at 484.199 kb on - strand, within GFF1820 at 484.199 kb on - strand, within GFF1820 at 484.199 kb on - strand, within GFF1820 at 484.210 kb on + strand, within GFF1820 at 484.211 kb on - strand, within GFF1820 at 484.222 kb on + strand, within GFF1820 at 484.222 kb on + strand, within GFF1820 at 484.222 kb on + strand, within GFF1820 at 484.222 kb on + strand, within GFF1820 at 484.222 kb on + strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820 at 484.223 kb on - strand, within GFF1820
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 481,454 + +0.2 481,454 + -0.4 481,454 + -1.0 481,454 + -1.9 481,454 + -0.9 481,454 + -0.9 481,454 + -2.4 481,476 - -0.1 481,545 + +0.0 481,546 - -0.2 481,595 + GFF1817 0.20 -0.1 481,595 + GFF1817 0.20 -0.2 481,596 - GFF1817 0.20 +0.0 481,596 - GFF1817 0.20 +1.2 481,673 + GFF1817 0.42 -0.4 481,673 + GFF1817 0.42 +0.5 481,703 + GFF1817 0.50 -1.3 481,704 - GFF1817 0.51 -0.2 481,730 + GFF1817 0.58 -1.5 481,730 + GFF1817 0.58 +0.2 481,820 + GFF1817 0.84 +1.2 481,820 + GFF1817 0.84 -1.4 481,820 + GFF1817 0.84 -0.7 481,820 + GFF1817 0.84 -2.2 481,820 + GFF1817 0.84 -0.6 481,820 + GFF1817 0.84 -2.6 481,820 + GFF1817 0.84 -0.6 481,821 - GFF1817 0.84 -1.1 481,821 - GFF1817 0.84 -0.1 481,821 - GFF1817 0.84 +0.1 481,821 - GFF1817 0.84 +0.4 481,821 - GFF1817 0.84 +0.1 481,853 + -0.3 481,853 + +1.0 481,853 + +0.6 481,853 + -0.1 481,853 + -0.9 481,854 - -0.8 481,854 - +1.4 481,854 - -0.5 481,854 - -0.7 481,862 + +0.8 481,863 - +1.1 482,152 + GFF1818 0.54 +0.1 482,152 + GFF1818 0.54 -0.2 482,152 + GFF1818 0.54 -0.2 482,152 + GFF1818 0.54 -0.1 482,152 + GFF1818 0.54 -0.5 482,152 + GFF1818 0.54 +0.8 482,152 + GFF1818 0.54 -0.7 482,153 - GFF1818 0.55 -0.5 482,153 - GFF1818 0.55 -0.4 482,153 - GFF1818 0.55 +0.2 482,153 - GFF1818 0.55 -0.4 482,153 - GFF1818 0.55 -0.5 482,153 - GFF1818 0.55 +0.4 482,176 + GFF1818 0.59 -1.8 482,176 + GFF1818 0.59 -0.6 482,177 - GFF1818 0.59 -0.2 482,177 - GFF1818 0.59 -0.1 482,177 - GFF1818 0.59 +0.6 482,177 - GFF1818 0.59 -0.9 482,177 - GFF1818 0.59 -0.0 482,177 - GFF1818 0.59 +0.3 482,363 + +0.0 482,363 + -0.7 482,364 - -0.1 482,388 + +0.8 482,388 + +0.1 482,424 + -0.4 482,720 + GFF1819 0.35 +0.2 483,059 + GFF1819 0.77 +1.3 483,059 + GFF1819 0.77 -0.2 483,059 + GFF1819 0.77 +0.2 483,059 + GFF1819 0.77 -0.2 483,060 - GFF1819 0.77 -0.6 483,060 - GFF1819 0.77 -0.2 483,060 - GFF1819 0.77 -0.3 483,060 - GFF1819 0.77 +0.6 483,060 - GFF1819 0.77 +0.3 483,060 - GFF1819 0.77 +0.2 483,060 - GFF1819 0.77 +0.5 483,383 + +0.1 483,383 + -0.0 483,469 + -0.4 483,469 + -1.0 483,469 + +0.8 483,469 + -0.8 483,469 + -0.7 483,469 + -0.1 483,469 + -1.2 483,470 - +0.4 483,470 - -0.1 483,470 - -0.1 483,470 - -0.2 483,472 + -0.6 483,472 + +0.1 483,472 + +0.1 483,473 - -0.1 483,547 + -0.2 483,548 - -0.6 483,649 + GFF1820 0.16 -0.4 483,649 + GFF1820 0.16 -0.4 483,649 + GFF1820 0.16 -0.2 483,650 - GFF1820 0.16 -0.0 483,650 - GFF1820 0.16 -1.4 483,650 - GFF1820 0.16 -0.1 483,664 + GFF1820 0.17 -1.0 483,664 + GFF1820 0.17 -0.4 483,664 + GFF1820 0.17 +0.4 483,664 + GFF1820 0.17 -0.8 483,664 + GFF1820 0.17 +1.5 483,664 + GFF1820 0.17 +1.6 483,664 + GFF1820 0.17 -0.5 483,664 + GFF1820 0.17 +1.0 483,665 - GFF1820 0.17 +0.2 483,665 - GFF1820 0.17 -0.1 483,709 + GFF1820 0.21 -1.3 483,721 + GFF1820 0.22 -1.4 483,721 + GFF1820 0.22 -1.5 483,721 + GFF1820 0.22 +0.0 483,721 + GFF1820 0.22 -1.2 483,721 + GFF1820 0.22 -1.4 483,721 + GFF1820 0.22 +1.2 483,721 + GFF1820 0.22 -0.2 483,721 + GFF1820 0.22 +1.2 483,722 - GFF1820 0.22 +0.2 483,722 - GFF1820 0.22 -1.1 483,722 - GFF1820 0.22 -1.2 483,735 + GFF1820 0.23 +0.6 483,735 + GFF1820 0.23 -0.2 483,763 + GFF1820 0.25 -0.8 483,763 + GFF1820 0.25 +0.3 483,763 + GFF1820 0.25 -0.3 483,764 - GFF1820 0.25 -1.0 483,764 - GFF1820 0.25 +0.1 483,764 - GFF1820 0.25 +0.2 483,764 - GFF1820 0.25 +0.5 483,764 - GFF1820 0.25 -0.8 483,784 + GFF1820 0.27 +0.3 483,785 - GFF1820 0.27 -0.5 483,785 - GFF1820 0.27 +1.4 483,785 - GFF1820 0.27 -1.4 483,871 + GFF1820 0.34 +1.4 483,871 + GFF1820 0.34 +0.3 483,872 - GFF1820 0.34 -0.4 483,872 - GFF1820 0.34 -0.7 483,872 - GFF1820 0.34 -1.0 484,156 + GFF1820 0.57 -0.5 484,156 + GFF1820 0.57 -1.3 484,156 + GFF1820 0.57 -0.0 484,156 + GFF1820 0.57 +1.1 484,156 + GFF1820 0.57 +1.2 484,156 + GFF1820 0.57 -0.7 484,157 - GFF1820 0.58 -0.3 484,157 - GFF1820 0.58 -2.6 484,157 - GFF1820 0.58 -1.7 484,199 - GFF1820 0.61 -1.8 484,199 - GFF1820 0.61 -0.2 484,199 - GFF1820 0.61 -0.3 484,210 + GFF1820 0.62 +1.0 484,211 - GFF1820 0.62 +0.1 484,222 + GFF1820 0.63 -0.6 484,222 + GFF1820 0.63 -0.3 484,222 + GFF1820 0.63 -1.2 484,222 + GFF1820 0.63 +0.2 484,222 + GFF1820 0.63 +3.4 484,223 - GFF1820 0.63 +0.4 484,223 - GFF1820 0.63 -0.2 484,223 - GFF1820 0.63 +0.6 484,223 - GFF1820 0.63 -1.3 484,223 - GFF1820 0.63 +0.0 484,223 - GFF1820 0.63 -0.5 484,223 - GFF1820 0.63 -0.5
Or see this region's nucleotide sequence