Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1739 and GFF1740 are separated by 9 nucleotides GFF1740 and GFF1741 are separated by 76 nucleotides GFF1741 and GFF1742 are separated by 116 nucleotides GFF1742 and GFF1743 are separated by 46 nucleotides
GFF1739 - Glutamate carboxypeptidase (EC 3.4.17.11), at 398,629 to 399,906
GFF1739
GFF1740 - Transcriptional regulator, LysR family, at 399,916 to 400,827
GFF1740
GFF1741 - UPF0324 inner membrane protein YeiH, at 400,904 to 401,956
GFF1741
GFF1742 - hypothetical protein, at 402,073 to 402,492
GFF1742
GFF1743 - Transcriptional regulator, LysR family, at 402,539 to 403,558
GFF1743
Position (kb) 400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2 at 400.476 kb on - strand, within GFF1740 at 400.500 kb on - strand, within GFF1740 at 400.514 kb on + strand, within GFF1740 at 400.515 kb on - strand, within GFF1740 at 400.891 kb on + strand at 400.907 kb on + strand at 400.907 kb on + strand at 400.907 kb on + strand at 400.907 kb on + strand at 400.907 kb on + strand at 400.907 kb on + strand at 400.908 kb on - strand at 400.908 kb on - strand at 400.908 kb on - strand at 401.268 kb on - strand, within GFF1741 at 401.268 kb on - strand, within GFF1741 at 401.447 kb on + strand, within GFF1741 at 401.448 kb on - strand, within GFF1741 at 401.465 kb on + strand, within GFF1741 at 401.465 kb on + strand, within GFF1741 at 401.465 kb on + strand, within GFF1741 at 402.347 kb on - strand, within GFF1742 at 402.560 kb on + strand at 402.560 kb on + strand at 402.614 kb on + strand at 402.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 400,476 - GFF1740 0.61 +0.3 400,500 - GFF1740 0.64 +0.4 400,514 + GFF1740 0.66 +2.1 400,515 - GFF1740 0.66 -0.3 400,891 + -0.3 400,907 + -0.8 400,907 + -0.8 400,907 + -0.2 400,907 + +1.1 400,907 + -2.2 400,907 + -0.8 400,908 - -0.1 400,908 - +1.1 400,908 - -0.5 401,268 - GFF1741 0.35 +0.4 401,268 - GFF1741 0.35 +0.2 401,447 + GFF1741 0.52 +0.0 401,448 - GFF1741 0.52 +0.1 401,465 + GFF1741 0.53 -0.1 401,465 + GFF1741 0.53 +1.4 401,465 + GFF1741 0.53 +1.5 402,347 - GFF1742 0.65 -0.6 402,560 + -0.6 402,560 + +0.5 402,614 + +0.2 402,614 + -0.9
Or see this region's nucleotide sequence