Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1733 and GFF1734 are separated by 39 nucleotides GFF1734 and GFF1735 are separated by 1 nucleotides GFF1735 and GFF1736 are separated by 85 nucleotides GFF1736 and GFF1737 are separated by 212 nucleotides
GFF1733 - Cell wall-associated hydrolases (invasion-associated proteins), at 393,612 to 394,115
GFF1733
GFF1734 - no description, at 394,155 to 394,514
GFF1734
GFF1735 - no description, at 394,516 to 394,635
GFF1735
GFF1736 - 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases, at 394,721 to 396,010
GFF1736
GFF1737 - Carbon starvation protein A, at 396,223 to 398,289
GFF1737
Position (kb) 394
395
396
397 Strain fitness (log2 ratio) -2
-1
0
1 at 393.801 kb on + strand, within GFF1733 at 393.858 kb on + strand, within GFF1733 at 393.858 kb on + strand, within GFF1733 at 393.859 kb on - strand, within GFF1733 at 393.864 kb on + strand, within GFF1733 at 393.865 kb on - strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.002 kb on + strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.003 kb on - strand, within GFF1733 at 394.078 kb on - strand at 394.078 kb on - strand at 394.435 kb on + strand, within GFF1734 at 394.435 kb on + strand, within GFF1734 at 394.435 kb on + strand, within GFF1734 at 394.489 kb on + strand at 394.666 kb on - strand at 394.724 kb on + strand at 395.304 kb on + strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.421 kb on + strand, within GFF1736 at 395.422 kb on - strand, within GFF1736 at 395.810 kb on + strand, within GFF1736 at 396.042 kb on + strand at 396.042 kb on + strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.073 kb on + strand at 396.073 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.356 kb on - strand at 396.356 kb on - strand at 396.368 kb on - strand at 396.373 kb on + strand at 396.373 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.377 kb on - strand at 396.461 kb on - strand, within GFF1737 at 396.559 kb on + strand, within GFF1737 at 396.560 kb on - strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.856 kb on + strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 397.010 kb on - strand, within GFF1737
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 393,801 + GFF1733 0.38 -1.0 393,858 + GFF1733 0.49 -0.7 393,858 + GFF1733 0.49 -0.1 393,859 - GFF1733 0.49 +0.1 393,864 + GFF1733 0.50 -0.2 393,865 - GFF1733 0.50 -0.2 394,002 + GFF1733 0.77 -0.8 394,002 + GFF1733 0.77 -0.8 394,002 + GFF1733 0.77 -1.3 394,002 + GFF1733 0.77 -1.4 394,002 + GFF1733 0.77 -0.2 394,002 + GFF1733 0.77 -0.1 394,002 + GFF1733 0.77 +0.7 394,002 + GFF1733 0.77 -0.2 394,002 + GFF1733 0.77 +0.8 394,003 - GFF1733 0.78 +1.2 394,003 - GFF1733 0.78 +0.3 394,003 - GFF1733 0.78 -0.3 394,003 - GFF1733 0.78 +0.2 394,003 - GFF1733 0.78 +0.3 394,003 - GFF1733 0.78 -0.1 394,078 - +0.9 394,078 - +0.4 394,435 + GFF1734 0.78 -0.6 394,435 + GFF1734 0.78 +0.2 394,435 + GFF1734 0.78 +0.4 394,489 + +0.4 394,666 - -0.2 394,724 + +0.8 395,304 + GFF1736 0.45 +0.1 395,305 - GFF1736 0.45 -1.6 395,305 - GFF1736 0.45 +0.8 395,421 + GFF1736 0.54 -0.4 395,422 - GFF1736 0.54 +1.1 395,810 + GFF1736 0.84 -0.1 396,042 + -0.2 396,042 + +0.4 396,043 - -0.1 396,043 - -0.1 396,043 - +0.5 396,043 - +0.8 396,073 + -0.9 396,073 + -0.9 396,355 + -1.1 396,355 + -0.8 396,355 + -0.6 396,355 + -0.8 396,355 + +0.5 396,356 - -1.8 396,356 - -0.5 396,368 - -1.0 396,373 + +0.2 396,373 + +0.6 396,376 + -0.1 396,376 + -1.8 396,376 + -0.8 396,376 + +0.2 396,376 + +0.5 396,377 - +0.9 396,461 - GFF1737 0.12 -0.8 396,559 + GFF1737 0.16 +0.9 396,560 - GFF1737 0.16 -0.7 396,847 + GFF1737 0.30 -0.2 396,847 + GFF1737 0.30 -0.7 396,847 + GFF1737 0.30 -0.1 396,848 - GFF1737 0.30 +0.2 396,848 - GFF1737 0.30 +1.4 396,848 - GFF1737 0.30 -0.3 396,856 + GFF1737 0.31 +0.3 396,857 - GFF1737 0.31 +0.3 396,857 - GFF1737 0.31 -0.4 396,857 - GFF1737 0.31 +0.3 397,010 - GFF1737 0.38 +1.2
Or see this region's nucleotide sequence