Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1664 and GFF1665 overlap by 4 nucleotides GFF1665 and GFF1666 overlap by 4 nucleotides GFF1666 and GFF1667 overlap by 4 nucleotides
GFF1664 - redox proteins related to the succinate dehydrogenases and fumarate reductases, at 307,969 to 309,384
GFF1664
GFF1665 - Nicotinamidase/isochorismatase family protein, at 309,381 to 309,995
GFF1665
GFF1666 - N-methylhydantoinase A (EC 3.5.2.14), at 309,992 to 312,049
GFF1666
GFF1667 - N-methylhydantoinase B (EC 3.5.2.14), at 312,046 to 313,728
GFF1667
Position (kb)
309
310
311
312
313 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 309.065 kb on + strand, within GFF1664 at 309.065 kb on + strand, within GFF1664 at 309.066 kb on - strand, within GFF1664 at 309.637 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 310.318 kb on - strand, within GFF1666 at 310.707 kb on + strand, within GFF1666 at 310.707 kb on + strand, within GFF1666 at 310.707 kb on + strand, within GFF1666 at 310.707 kb on + strand, within GFF1666 at 310.708 kb on - strand, within GFF1666 at 310.708 kb on - strand, within GFF1666 at 310.708 kb on - strand, within GFF1666 at 311.391 kb on + strand, within GFF1666 at 311.391 kb on + strand, within GFF1666 at 311.553 kb on + strand, within GFF1666 at 312.083 kb on + strand at 312.083 kb on + strand at 312.083 kb on + strand at 312.083 kb on + strand at 312.083 kb on + strand at 312.084 kb on - strand at 312.084 kb on - strand at 312.084 kb on - strand at 312.632 kb on + strand, within GFF1667 at 312.633 kb on - strand, within GFF1667 at 312.839 kb on + strand, within GFF1667 at 312.839 kb on + strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 313.007 kb on + strand, within GFF1667
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 309,065 + GFF1664 0.77 -0.0 309,065 + GFF1664 0.77 +0.2 309,066 - GFF1664 0.77 -0.4 309,637 + GFF1665 0.42 +0.3 309,907 + GFF1665 0.86 -0.2 309,907 + GFF1665 0.86 +0.0 309,907 + GFF1665 0.86 +0.5 310,318 - GFF1666 0.16 -0.5 310,707 + GFF1666 0.35 -0.3 310,707 + GFF1666 0.35 -0.7 310,707 + GFF1666 0.35 -0.1 310,707 + GFF1666 0.35 -0.2 310,708 - GFF1666 0.35 -0.2 310,708 - GFF1666 0.35 -0.6 310,708 - GFF1666 0.35 +0.1 311,391 + GFF1666 0.68 +0.4 311,391 + GFF1666 0.68 -2.0 311,553 + GFF1666 0.76 -1.5 312,083 + -0.2 312,083 + -1.3 312,083 + -0.2 312,083 + -0.4 312,083 + +0.2 312,084 - +0.7 312,084 - +0.1 312,084 - +0.2 312,632 + GFF1667 0.35 +0.6 312,633 - GFF1667 0.35 -0.0 312,839 + GFF1667 0.47 +0.2 312,839 + GFF1667 0.47 +0.2 312,840 - GFF1667 0.47 -0.7 312,840 - GFF1667 0.47 -0.8 312,840 - GFF1667 0.47 +0.1 312,840 - GFF1667 0.47 -0.9 312,840 - GFF1667 0.47 +0.4 313,007 + GFF1667 0.57 +1.8
Or see this region's nucleotide sequence