Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1656 and GFF1657 are separated by 40 nucleotides GFF1657 and GFF1658 are separated by 91 nucleotides GFF1658 and GFF1659 overlap by 4 nucleotides
GFF1656 - hypothetical protein, at 296,441 to 298,957
GFF1656
GFF1657 - Nitronate monooxygenase (EC 1.13.12.16), at 298,998 to 300,119
GFF1657
GFF1658 - Transcriptional regulator, LysR family, at 300,211 to 301,086
GFF1658
GFF1659 - Uncharacterized MFS-type transporter, at 301,083 to 302,249
GFF1659
Position (kb) 298
299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 298.196 kb on + strand, within GFF1656 at 298.380 kb on + strand, within GFF1656 at 298.380 kb on + strand, within GFF1656 at 298.380 kb on + strand, within GFF1656 at 298.380 kb on + strand, within GFF1656 at 298.381 kb on - strand, within GFF1656 at 298.381 kb on - strand, within GFF1656 at 298.571 kb on + strand, within GFF1656 at 298.571 kb on + strand, within GFF1656 at 298.571 kb on + strand, within GFF1656 at 298.572 kb on - strand, within GFF1656 at 298.572 kb on - strand, within GFF1656 at 298.646 kb on + strand, within GFF1656 at 298.646 kb on + strand, within GFF1656 at 298.647 kb on - strand, within GFF1656 at 298.647 kb on - strand, within GFF1656 at 298.727 kb on + strand at 298.868 kb on + strand at 298.868 kb on + strand at 298.869 kb on - strand at 298.869 kb on - strand at 298.955 kb on + strand at 298.955 kb on + strand at 298.955 kb on + strand at 298.955 kb on + strand at 298.956 kb on - strand at 298.956 kb on - strand at 298.956 kb on - strand at 299.207 kb on - strand, within GFF1657 at 299.207 kb on - strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.788 kb on + strand, within GFF1657 at 299.789 kb on - strand, within GFF1657 at 299.789 kb on - strand, within GFF1657 at 299.789 kb on - strand, within GFF1657 at 299.789 kb on - strand, within GFF1657 at 300.138 kb on + strand at 300.139 kb on - strand at 300.183 kb on + strand at 300.409 kb on + strand, within GFF1658 at 300.553 kb on + strand, within GFF1658 at 300.814 kb on + strand, within GFF1658 at 301.030 kb on + strand at 301.030 kb on + strand at 301.031 kb on - strand at 301.031 kb on - strand at 301.031 kb on - strand at 301.031 kb on - strand at 301.031 kb on - strand at 301.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 298,196 + GFF1656 0.70 -0.0 298,380 + GFF1656 0.77 -0.8 298,380 + GFF1656 0.77 +0.2 298,380 + GFF1656 0.77 +0.3 298,380 + GFF1656 0.77 -0.2 298,381 - GFF1656 0.77 -0.2 298,381 - GFF1656 0.77 -0.3 298,571 + GFF1656 0.85 +0.1 298,571 + GFF1656 0.85 +0.7 298,571 + GFF1656 0.85 -0.1 298,572 - GFF1656 0.85 -0.1 298,572 - GFF1656 0.85 +1.0 298,646 + GFF1656 0.88 -1.0 298,646 + GFF1656 0.88 -0.0 298,647 - GFF1656 0.88 -1.9 298,647 - GFF1656 0.88 -0.6 298,727 + +0.2 298,868 + -0.2 298,868 + -0.3 298,869 - +0.0 298,869 - -1.1 298,955 + +0.2 298,955 + +0.3 298,955 + -0.3 298,955 + -0.4 298,956 - +0.4 298,956 - +0.1 298,956 - -0.4 299,207 - GFF1657 0.19 -0.6 299,207 - GFF1657 0.19 +0.2 299,788 + GFF1657 0.70 -0.1 299,788 + GFF1657 0.70 -0.7 299,788 + GFF1657 0.70 -1.5 299,788 + GFF1657 0.70 -0.5 299,788 + GFF1657 0.70 -0.1 299,788 + GFF1657 0.70 -1.4 299,788 + GFF1657 0.70 -0.1 299,788 + GFF1657 0.70 -2.2 299,789 - GFF1657 0.70 -0.7 299,789 - GFF1657 0.70 -0.0 299,789 - GFF1657 0.70 -0.2 299,789 - GFF1657 0.70 -3.1 300,138 + +0.4 300,139 - +0.1 300,183 + -0.7 300,409 + GFF1658 0.23 -0.2 300,553 + GFF1658 0.39 -0.1 300,814 + GFF1658 0.69 +2.0 301,030 + -0.3 301,030 + +0.3 301,031 - -0.4 301,031 - -0.5 301,031 - -1.7 301,031 - -0.1 301,031 - -0.7 301,031 - -0.5
Or see this region's nucleotide sequence