Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1573 and GFF1574 are separated by 201 nucleotides GFF1574 and GFF1575 overlap by 4 nucleotides GFF1575 and GFF1576 are separated by 19 nucleotides
GFF1573 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 173,540 to 174,274
GFF1573
GFF1574 - Alpha-aminoadipate aminotransferase (EC 2.6.1.39) @ Leucine transaminase (EC 2.6.1.6) @ Valine transaminase, at 174,476 to 175,687
GFF1574
GFF1575 - BUG/TctC family periplasmic protein, at 175,684 to 176,655
GFF1575
GFF1576 - 2-pyrone-4,6-dicarboxylic acid hydrolase (EC 3.1.1.57), at 176,675 to 177,592
GFF1576
Position (kb) 174
175
176 Strain fitness (log2 ratio) -2
-1
0
1 at 174.018 kb on - strand, within GFF1573 at 174.018 kb on - strand, within GFF1573 at 174.681 kb on - strand, within GFF1574 at 174.860 kb on + strand, within GFF1574 at 174.861 kb on - strand, within GFF1574 at 174.890 kb on + strand, within GFF1574 at 174.902 kb on + strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.283 kb on + strand, within GFF1574 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.150 kb on - strand, within GFF1575 at 176.150 kb on - strand, within GFF1575 at 176.606 kb on - strand at 176.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 174,018 - GFF1573 0.65 +0.1 174,018 - GFF1573 0.65 -0.8 174,681 - GFF1574 0.17 -1.4 174,860 + GFF1574 0.32 -1.3 174,861 - GFF1574 0.32 +0.9 174,890 + GFF1574 0.34 -0.7 174,902 + GFF1574 0.35 -0.8 174,978 - GFF1574 0.41 -0.6 174,978 - GFF1574 0.41 -0.4 174,978 - GFF1574 0.41 -0.2 175,208 + GFF1574 0.60 -2.0 175,208 + GFF1574 0.60 -2.4 175,208 + GFF1574 0.60 -0.4 175,208 + GFF1574 0.60 -0.2 175,208 + GFF1574 0.60 -0.4 175,209 - GFF1574 0.60 -0.5 175,209 - GFF1574 0.60 +0.0 175,209 - GFF1574 0.60 -0.1 175,209 - GFF1574 0.60 -1.0 175,283 + GFF1574 0.67 +0.2 176,149 + GFF1575 0.48 +0.2 176,149 + GFF1575 0.48 -0.7 176,149 + GFF1575 0.48 -0.2 176,149 + GFF1575 0.48 -0.1 176,150 - GFF1575 0.48 -0.3 176,150 - GFF1575 0.48 +0.2 176,606 - +0.2 176,606 - -0.6
Or see this region's nucleotide sequence