Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF155 and GFF156 are separated by 16 nucleotides GFF156 and GFF157 overlap by 4 nucleotides GFF157 and GFF158 overlap by 4 nucleotides GFF158 and GFF159 overlap by 13 nucleotides
GFF155 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 183,212 to 184,783
GFF155
GFF156 - Glycosyl transferase, group 2 family, at 184,800 to 185,498
GFF156
GFF157 - Methyltransferase type 12, at 185,495 to 186,100
GFF157
GFF158 - LmbE-like protein, at 186,097 to 186,858
GFF158
GFF159 - Acyl-CoA dehydrogenase/oxidase domain protein, at 186,846 to 187,865
GFF159
Position (kb) 185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 184.499 kb on + strand, within GFF155 at 184.500 kb on - strand, within GFF155 at 184.500 kb on - strand, within GFF155 at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.657 kb on + strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.658 kb on - strand at 184.907 kb on + strand, within GFF156 at 184.908 kb on - strand, within GFF156 at 184.908 kb on - strand, within GFF156 at 185.083 kb on + strand, within GFF156 at 185.083 kb on + strand, within GFF156 at 185.084 kb on - strand, within GFF156 at 185.084 kb on - strand, within GFF156 at 185.746 kb on - strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.959 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 186.021 kb on + strand, within GFF157 at 186.022 kb on - strand, within GFF157 at 186.042 kb on + strand at 186.043 kb on - strand at 186.043 kb on - strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.078 kb on + strand at 186.078 kb on + strand at 186.116 kb on + strand at 186.116 kb on + strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.847 kb on + strand at 186.848 kb on - strand at 186.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 184,499 + GFF155 0.82 -1.7 184,500 - GFF155 0.82 +0.5 184,500 - GFF155 0.82 -0.9 184,657 + -0.3 184,657 + +0.0 184,657 + -0.4 184,657 + -0.4 184,657 + +0.5 184,658 - -1.0 184,658 - -0.6 184,658 - -0.9 184,658 - -0.8 184,658 - -1.9 184,907 + GFF156 0.15 +1.3 184,908 - GFF156 0.15 -0.7 184,908 - GFF156 0.15 +0.2 185,083 + GFF156 0.40 +0.9 185,083 + GFF156 0.40 -0.5 185,084 - GFF156 0.41 -0.6 185,084 - GFF156 0.41 -0.3 185,746 - GFF157 0.41 +1.6 185,958 + GFF157 0.76 +0.1 185,958 + GFF157 0.76 -0.0 185,959 - GFF157 0.77 -0.8 185,971 - GFF157 0.79 +1.0 185,971 - GFF157 0.79 +1.0 186,021 + GFF157 0.87 -0.1 186,022 - GFF157 0.87 -0.1 186,042 + -0.1 186,043 - -0.2 186,043 - -2.9 186,063 + -0.2 186,063 + +1.5 186,063 + -0.6 186,063 + -0.7 186,064 - -0.9 186,064 - +0.6 186,064 - -1.3 186,078 + -0.8 186,078 + -2.4 186,116 + +0.1 186,116 + -0.8 186,117 - -0.8 186,117 - -0.3 186,117 - +1.1 186,117 - -2.5 186,847 + -0.1 186,848 - +0.3 186,848 - -1.7
Or see this region's nucleotide sequence