Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1458 and GFF1459 are separated by 76 nucleotides GFF1459 and GFF1460 are separated by 114 nucleotides GFF1460 and GFF1461 are separated by 2 nucleotides GFF1461 and GFF1462 overlap by 4 nucleotides
GFF1458 - no description, at 50,882 to 51,004
GFF1458
GFF1459 - Glutathione S-transferase (EC 2.5.1.18), at 51,081 to 51,731
GFF1459
GFF1460 - Enoyl-CoA hydratase/isomerase, at 51,846 to 52,592
GFF1460
GFF1461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 52,595 to 53,401
GFF1461
GFF1462 - L-carnitine dehydratase/bile acid-inducible protein F, at 53,398 to 54,600
GFF1462
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 50.967 kb on + strand, within GFF1458 at 50.968 kb on - strand, within GFF1458 at 50.968 kb on - strand, within GFF1458 at 51.000 kb on + strand at 51.000 kb on + strand at 51.020 kb on + strand at 51.021 kb on - strand at 51.021 kb on - strand at 51.058 kb on - strand at 51.095 kb on - strand at 51.200 kb on + strand, within GFF1459 at 51.200 kb on + strand, within GFF1459 at 51.201 kb on - strand, within GFF1459 at 51.201 kb on - strand, within GFF1459 at 51.301 kb on + strand, within GFF1459 at 51.301 kb on + strand, within GFF1459 at 51.302 kb on - strand, within GFF1459 at 51.355 kb on + strand, within GFF1459 at 51.356 kb on - strand, within GFF1459 at 51.379 kb on + strand, within GFF1459 at 51.394 kb on + strand, within GFF1459 at 51.415 kb on + strand, within GFF1459 at 51.415 kb on + strand, within GFF1459 at 51.544 kb on + strand, within GFF1459 at 51.544 kb on + strand, within GFF1459 at 51.544 kb on + strand, within GFF1459 at 51.545 kb on - strand, within GFF1459 at 51.545 kb on - strand, within GFF1459 at 52.199 kb on - strand, within GFF1460 at 52.297 kb on + strand, within GFF1460 at 52.298 kb on - strand, within GFF1460 at 52.298 kb on - strand, within GFF1460 at 52.405 kb on + strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.653 kb on + strand at 52.653 kb on + strand at 52.791 kb on + strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.947 kb on + strand, within GFF1461 at 52.948 kb on - strand, within GFF1461 at 52.979 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 50,967 + GFF1458 0.69 +0.1 50,968 - GFF1458 0.70 -0.3 50,968 - GFF1458 0.70 +0.3 51,000 + -0.7 51,000 + -1.8 51,020 + +0.1 51,021 - -0.6 51,021 - -0.8 51,058 - -0.9 51,095 - +0.2 51,200 + GFF1459 0.18 -1.4 51,200 + GFF1459 0.18 +0.7 51,201 - GFF1459 0.18 +0.5 51,201 - GFF1459 0.18 -0.1 51,301 + GFF1459 0.34 -0.1 51,301 + GFF1459 0.34 -0.1 51,302 - GFF1459 0.34 -0.2 51,355 + GFF1459 0.42 -0.4 51,356 - GFF1459 0.42 +0.1 51,379 + GFF1459 0.46 -0.5 51,394 + GFF1459 0.48 -0.6 51,415 + GFF1459 0.51 +0.2 51,415 + GFF1459 0.51 +0.3 51,544 + GFF1459 0.71 +1.0 51,544 + GFF1459 0.71 -0.6 51,544 + GFF1459 0.71 -0.2 51,545 - GFF1459 0.71 -0.6 51,545 - GFF1459 0.71 +0.7 52,199 - GFF1460 0.47 +0.7 52,297 + GFF1460 0.60 +0.9 52,298 - GFF1460 0.61 +2.4 52,298 - GFF1460 0.61 +0.6 52,405 + GFF1460 0.75 -0.2 52,421 - GFF1460 0.77 +0.3 52,421 - GFF1460 0.77 +0.3 52,653 + -1.1 52,653 + +0.7 52,791 + GFF1461 0.24 -0.7 52,792 - GFF1461 0.24 -0.2 52,792 - GFF1461 0.24 -0.6 52,792 - GFF1461 0.24 -0.3 52,947 + GFF1461 0.44 +0.1 52,948 - GFF1461 0.44 -0.7 52,979 + GFF1461 0.48 -0.2 53,130 + GFF1461 0.66 -0.8 53,130 + GFF1461 0.66 +0.2 53,131 - GFF1461 0.66 -0.2 53,131 - GFF1461 0.66 -0.8 53,131 - GFF1461 0.66 -0.2 53,510 + +0.0
Or see this region's nucleotide sequence