Strain Fitness in Variovorax sp. SCN45 around GFF1397

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1396 and GFF1397 are separated by 6 nucleotidesGFF1397 and GFF1398 are separated by 42 nucleotides GFF1396 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 744,048 to 745,085 GFF1396 GFF1397 - TRAP-type C4-dicarboxylate transport system, large permease component, at 745,092 to 746,990 GFF1397 GFF1398 - Transcriptional regulator, LacI family, at 747,033 to 748,082 GFF1398 Position (kb) 745 746 747Strain fitness (log2 ratio) -2 -1 0 1 2at 744.133 kb on - strandat 744.274 kb on - strand, within GFF1396at 744.274 kb on - strand, within GFF1396at 744.408 kb on + strand, within GFF1396at 744.408 kb on + strand, within GFF1396at 744.423 kb on + strand, within GFF1396at 744.423 kb on + strand, within GFF1396at 744.423 kb on + strand, within GFF1396at 744.424 kb on - strand, within GFF1396at 744.424 kb on - strand, within GFF1396at 744.424 kb on - strand, within GFF1396at 744.424 kb on - strand, within GFF1396at 744.525 kb on + strand, within GFF1396at 744.543 kb on + strand, within GFF1396at 744.543 kb on + strand, within GFF1396at 744.543 kb on + strand, within GFF1396at 744.543 kb on + strand, within GFF1396at 744.543 kb on + strand, within GFF1396at 744.544 kb on - strand, within GFF1396at 744.544 kb on - strand, within GFF1396at 744.544 kb on - strand, within GFF1396at 744.675 kb on + strand, within GFF1396at 744.675 kb on + strand, within GFF1396at 744.675 kb on + strand, within GFF1396at 744.676 kb on - strand, within GFF1396at 744.676 kb on - strand, within GFF1396at 744.676 kb on - strand, within GFF1396at 744.676 kb on - strand, within GFF1396at 745.036 kb on - strandat 745.036 kb on - strandat 745.207 kb on - strandat 745.269 kb on + strandat 745.270 kb on - strandat 745.506 kb on + strand, within GFF1397at 745.506 kb on + strand, within GFF1397at 745.507 kb on - strand, within GFF1397at 745.507 kb on - strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.545 kb on + strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.546 kb on - strand, within GFF1397at 745.833 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.866 kb on + strand, within GFF1397at 745.867 kb on - strand, within GFF1397at 745.867 kb on - strand, within GFF1397at 745.867 kb on - strand, within GFF1397at 746.277 kb on + strand, within GFF1397at 746.277 kb on + strand, within GFF1397at 746.277 kb on + strand, within GFF1397at 746.277 kb on + strand, within GFF1397at 746.278 kb on - strand, within GFF1397at 746.278 kb on - strand, within GFF1397at 746.278 kb on - strand, within GFF1397at 746.278 kb on - strand, within GFF1397at 746.442 kb on + strand, within GFF1397at 746.442 kb on + strand, within GFF1397at 746.442 kb on + strand, within GFF1397at 746.443 kb on - strand, within GFF1397at 746.443 kb on - strand, within GFF1397at 746.443 kb on - strand, within GFF1397at 746.443 kb on - strand, within GFF1397at 746.602 kb on - strand, within GFF1397at 746.602 kb on - strand, within GFF1397at 746.904 kb on + strandat 746.905 kb on - strandat 747.255 kb on + strand, within GFF1398at 747.255 kb on + strand, within GFF1398at 747.255 kb on + strand, within GFF1398at 747.256 kb on - strand, within GFF1398at 747.256 kb on - strand, within GFF1398at 747.330 kb on + strand, within GFF1398at 747.331 kb on - strand, within GFF1398at 747.469 kb on - strand, within GFF1398at 747.469 kb on - strand, within GFF1398at 747.469 kb on - strand, within GFF1398

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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744,133 - -0.4
744,274 - GFF1396 0.22 -0.6
744,274 - GFF1396 0.22 -1.0
744,408 + GFF1396 0.35 -0.1
744,408 + GFF1396 0.35 -0.1
744,423 + GFF1396 0.36 -1.0
744,423 + GFF1396 0.36 +1.8
744,423 + GFF1396 0.36 -1.2
744,424 - GFF1396 0.36 -0.3
744,424 - GFF1396 0.36 +1.4
744,424 - GFF1396 0.36 +0.5
744,424 - GFF1396 0.36 +0.1
744,525 + GFF1396 0.46 -0.3
744,543 + GFF1396 0.48 +0.4
744,543 + GFF1396 0.48 +0.1
744,543 + GFF1396 0.48 -0.0
744,543 + GFF1396 0.48 -0.0
744,543 + GFF1396 0.48 -0.6
744,544 - GFF1396 0.48 -0.2
744,544 - GFF1396 0.48 -0.3
744,544 - GFF1396 0.48 -0.8
744,675 + GFF1396 0.60 -1.8
744,675 + GFF1396 0.60 -0.6
744,675 + GFF1396 0.60 -0.1
744,676 - GFF1396 0.61 -0.7
744,676 - GFF1396 0.61 -1.8
744,676 - GFF1396 0.61 +0.2
744,676 - GFF1396 0.61 -0.4
745,036 - -1.6
745,036 - -2.2
745,207 - -0.7
745,269 + -1.4
745,270 - -0.3
745,506 + GFF1397 0.22 -1.4
745,506 + GFF1397 0.22 +0.0
745,507 - GFF1397 0.22 -0.1
745,507 - GFF1397 0.22 -1.6
745,545 + GFF1397 0.24 -1.6
745,545 + GFF1397 0.24 +0.4
745,545 + GFF1397 0.24 -0.9
745,545 + GFF1397 0.24 +1.0
745,545 + GFF1397 0.24 -0.3
745,545 + GFF1397 0.24 +0.7
745,545 + GFF1397 0.24 +0.3
745,545 + GFF1397 0.24 -1.8
745,546 - GFF1397 0.24 -0.8
745,546 - GFF1397 0.24 -0.5
745,546 - GFF1397 0.24 +0.5
745,546 - GFF1397 0.24 +0.2
745,546 - GFF1397 0.24 -0.7
745,546 - GFF1397 0.24 -0.6
745,546 - GFF1397 0.24 -0.1
745,546 - GFF1397 0.24 -0.4
745,833 + GFF1397 0.39 -1.8
745,866 + GFF1397 0.41 -2.0
745,866 + GFF1397 0.41 +0.1
745,866 + GFF1397 0.41 +0.2
745,866 + GFF1397 0.41 +0.1
745,866 + GFF1397 0.41 +0.2
745,866 + GFF1397 0.41 -0.1
745,867 - GFF1397 0.41 -1.1
745,867 - GFF1397 0.41 +1.8
745,867 - GFF1397 0.41 -0.3
746,277 + GFF1397 0.62 -1.2
746,277 + GFF1397 0.62 -0.7
746,277 + GFF1397 0.62 -2.0
746,277 + GFF1397 0.62 -0.6
746,278 - GFF1397 0.62 -0.2
746,278 - GFF1397 0.62 +0.2
746,278 - GFF1397 0.62 -1.0
746,278 - GFF1397 0.62 -0.6
746,442 + GFF1397 0.71 -1.6
746,442 + GFF1397 0.71 -0.3
746,442 + GFF1397 0.71 -1.0
746,443 - GFF1397 0.71 -0.4
746,443 - GFF1397 0.71 +0.4
746,443 - GFF1397 0.71 +0.1
746,443 - GFF1397 0.71 -0.5
746,602 - GFF1397 0.80 -0.1
746,602 - GFF1397 0.80 -0.8
746,904 + -0.5
746,905 - -0.3
747,255 + GFF1398 0.21 -0.4
747,255 + GFF1398 0.21 +0.1
747,255 + GFF1398 0.21 +0.2
747,256 - GFF1398 0.21 -0.8
747,256 - GFF1398 0.21 -0.9
747,330 + GFF1398 0.28 +0.2
747,331 - GFF1398 0.28 -0.5
747,469 - GFF1398 0.42 +0.0
747,469 - GFF1398 0.42 +0.0
747,469 - GFF1398 0.42 +0.5

Or see this region's nucleotide sequence