Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1279 and GFF1280 are separated by 13 nucleotides GFF1280 and GFF1281 overlap by 10 nucleotides GFF1281 and GFF1282 are separated by 45 nucleotides GFF1282 and GFF1283 are separated by 68 nucleotides
GFF1279 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 608,302 to 609,135
GFF1279
GFF1280 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 609,149 to 609,856
GFF1280
GFF1281 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 609,847 to 610,578
GFF1281
GFF1282 - Chromate reductase (EC 1.6.5.2), at 610,624 to 611,178
GFF1282
GFF1283 - FMN-dependent NADH-azoreductase (EC 1.7.1.6), at 611,247 to 611,894
GFF1283
Position (kb)
609
610
611 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 608.942 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.954 kb on - strand, within GFF1279 at 609.052 kb on + strand at 609.052 kb on + strand at 609.052 kb on + strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.485 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.710 kb on + strand, within GFF1280 at 609.711 kb on - strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.884 kb on - strand at 609.884 kb on - strand at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.094 kb on - strand, within GFF1281 at 610.094 kb on - strand, within GFF1281 at 610.094 kb on - strand, within GFF1281 at 610.177 kb on + strand, within GFF1281 at 610.177 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.363 kb on + strand, within GFF1281 at 610.364 kb on - strand, within GFF1281 at 610.364 kb on - strand, within GFF1281 at 610.364 kb on - strand, within GFF1281 at 610.364 kb on - strand, within GFF1281 at 610.364 kb on - strand, within GFF1281 at 610.365 kb on + strand, within GFF1281 at 610.365 kb on + strand, within GFF1281 at 610.366 kb on - strand, within GFF1281 at 610.366 kb on - strand, within GFF1281 at 610.366 kb on - strand, within GFF1281 at 610.426 kb on + strand, within GFF1281 at 610.463 kb on - strand, within GFF1281 at 610.463 kb on - strand, within GFF1281 at 610.463 kb on - strand, within GFF1281 at 610.463 kb on - strand, within GFF1281 at 610.463 kb on - strand, within GFF1281 at 610.675 kb on + strand at 610.675 kb on + strand at 610.675 kb on + strand at 610.675 kb on + strand at 610.675 kb on + strand at 610.676 kb on - strand at 610.676 kb on - strand at 610.676 kb on - strand at 610.762 kb on + strand, within GFF1282 at 610.762 kb on + strand, within GFF1282 at 610.763 kb on - strand, within GFF1282 at 610.763 kb on - strand, within GFF1282 at 610.763 kb on - strand, within GFF1282 at 610.858 kb on + strand, within GFF1282 at 610.858 kb on + strand, within GFF1282 at 610.858 kb on + strand, within GFF1282 at 610.859 kb on - strand, within GFF1282 at 610.859 kb on - strand, within GFF1282 at 610.859 kb on - strand, within GFF1282 at 611.020 kb on + strand, within GFF1282 at 611.020 kb on + strand, within GFF1282 at 611.021 kb on - strand, within GFF1282 at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.146 kb on + strand at 611.147 kb on - strand at 611.147 kb on - strand at 611.147 kb on - strand at 611.147 kb on - strand at 611.147 kb on - strand at 611.147 kb on - strand at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.419 kb on + strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.420 kb on - strand, within GFF1283 at 611.464 kb on + strand, within GFF1283 at 611.465 kb on - strand, within GFF1283 at 611.465 kb on - strand, within GFF1283 at 611.465 kb on - strand, within GFF1283 at 611.536 kb on + strand, within GFF1283 at 611.537 kb on - strand, within GFF1283 at 611.537 kb on - strand, within GFF1283
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 608,942 + GFF1279 0.77 -0.1 608,953 + GFF1279 0.78 +0.1 608,953 + GFF1279 0.78 -1.3 608,953 + GFF1279 0.78 -2.5 608,954 - GFF1279 0.78 -0.5 609,052 + -1.0 609,052 + -0.3 609,052 + -1.1 609,053 - -1.4 609,053 - -0.3 609,053 - -0.0 609,053 - -0.2 609,329 + GFF1280 0.25 -0.6 609,329 + GFF1280 0.25 -0.6 609,329 + GFF1280 0.25 -1.7 609,329 + GFF1280 0.25 -0.1 609,329 + GFF1280 0.25 -0.2 609,329 + GFF1280 0.25 -0.1 609,330 - GFF1280 0.26 +0.4 609,330 - GFF1280 0.26 -0.2 609,330 - GFF1280 0.26 -0.5 609,330 - GFF1280 0.26 -0.7 609,330 - GFF1280 0.26 -0.8 609,330 - GFF1280 0.26 -0.0 609,330 - GFF1280 0.26 +0.5 609,380 + GFF1280 0.33 +0.1 609,380 + GFF1280 0.33 -0.2 609,380 + GFF1280 0.33 -0.6 609,428 + GFF1280 0.39 +0.4 609,428 + GFF1280 0.39 -0.1 609,428 + GFF1280 0.39 -0.1 609,428 + GFF1280 0.39 +0.4 609,429 - GFF1280 0.40 +0.5 609,429 - GFF1280 0.40 +0.1 609,429 - GFF1280 0.40 -1.0 609,429 - GFF1280 0.40 -2.3 609,429 - GFF1280 0.40 +0.1 609,485 + GFF1280 0.47 -0.8 609,497 + GFF1280 0.49 -0.9 609,497 + GFF1280 0.49 -1.3 609,497 + GFF1280 0.49 -0.4 609,497 + GFF1280 0.49 -0.8 609,497 + GFF1280 0.49 +0.1 609,497 + GFF1280 0.49 -0.8 609,497 + GFF1280 0.49 -1.0 609,497 + GFF1280 0.49 +0.0 609,498 - GFF1280 0.49 +1.4 609,498 - GFF1280 0.49 -0.0 609,498 - GFF1280 0.49 -1.3 609,498 - GFF1280 0.49 -0.3 609,498 - GFF1280 0.49 -0.4 609,650 + GFF1280 0.71 -0.2 609,650 + GFF1280 0.71 +1.8 609,650 + GFF1280 0.71 -0.2 609,650 + GFF1280 0.71 +0.0 609,650 + GFF1280 0.71 +0.2 609,650 + GFF1280 0.71 +1.0 609,650 + GFF1280 0.71 -0.6 609,650 + GFF1280 0.71 -1.1 609,651 - GFF1280 0.71 +0.4 609,651 - GFF1280 0.71 -0.6 609,651 - GFF1280 0.71 -2.5 609,651 - GFF1280 0.71 +0.4 609,651 - GFF1280 0.71 +1.4 609,651 - GFF1280 0.71 -1.5 609,651 - GFF1280 0.71 +0.5 609,710 + GFF1280 0.79 +0.4 609,711 - GFF1280 0.79 +0.4 609,779 + GFF1280 0.89 -0.2 609,779 + GFF1280 0.89 -1.3 609,779 + GFF1280 0.89 -1.4 609,780 - GFF1280 0.89 -1.7 609,780 - GFF1280 0.89 +0.8 609,780 - GFF1280 0.89 +1.0 609,780 - GFF1280 0.89 -0.2 609,884 - -1.5 609,884 - +1.0 610,093 + GFF1281 0.34 +0.4 610,093 + GFF1281 0.34 -1.1 610,093 + GFF1281 0.34 +0.1 610,093 + GFF1281 0.34 -0.2 610,093 + GFF1281 0.34 +0.5 610,093 + GFF1281 0.34 -0.2 610,094 - GFF1281 0.34 -0.5 610,094 - GFF1281 0.34 -0.6 610,094 - GFF1281 0.34 +2.4 610,177 + GFF1281 0.45 +0.8 610,177 + GFF1281 0.45 -0.3 610,363 + GFF1281 0.70 +0.1 610,363 + GFF1281 0.70 -0.2 610,363 + GFF1281 0.70 +0.9 610,363 + GFF1281 0.70 -0.5 610,363 + GFF1281 0.70 +0.4 610,363 + GFF1281 0.70 -0.5 610,363 + GFF1281 0.70 +0.1 610,363 + GFF1281 0.70 -0.3 610,364 - GFF1281 0.71 -0.3 610,364 - GFF1281 0.71 -0.5 610,364 - GFF1281 0.71 -0.8 610,364 - GFF1281 0.71 +0.8 610,364 - GFF1281 0.71 +0.2 610,365 + GFF1281 0.71 -0.4 610,365 + GFF1281 0.71 -1.0 610,366 - GFF1281 0.71 -0.0 610,366 - GFF1281 0.71 +0.0 610,366 - GFF1281 0.71 -0.1 610,426 + GFF1281 0.79 +1.4 610,463 - GFF1281 0.84 +1.8 610,463 - GFF1281 0.84 +0.2 610,463 - GFF1281 0.84 -0.5 610,463 - GFF1281 0.84 +0.4 610,463 - GFF1281 0.84 +0.3 610,675 + -0.2 610,675 + -0.6 610,675 + -0.2 610,675 + -0.0 610,675 + -0.2 610,676 - -0.1 610,676 - -0.2 610,676 - -0.4 610,762 + GFF1282 0.25 +0.3 610,762 + GFF1282 0.25 +0.1 610,763 - GFF1282 0.25 -0.5 610,763 - GFF1282 0.25 -1.0 610,763 - GFF1282 0.25 +0.4 610,858 + GFF1282 0.42 +0.3 610,858 + GFF1282 0.42 -0.1 610,858 + GFF1282 0.42 -0.3 610,859 - GFF1282 0.42 +0.4 610,859 - GFF1282 0.42 -0.6 610,859 - GFF1282 0.42 -0.1 611,020 + GFF1282 0.71 -0.1 611,020 + GFF1282 0.71 +0.0 611,021 - GFF1282 0.72 +1.8 611,146 + -1.2 611,146 + -1.0 611,146 + +0.2 611,146 + +0.7 611,146 + -0.5 611,146 + -1.2 611,146 + -0.5 611,146 + -1.6 611,146 + -0.7 611,146 + +0.5 611,146 + -1.8 611,146 + +0.0 611,146 + -2.3 611,146 + -0.5 611,146 + -2.0 611,146 + +1.1 611,147 - +0.0 611,147 - -1.6 611,147 - -0.2 611,147 - -1.2 611,147 - +1.0 611,147 - -0.5 611,419 + GFF1283 0.27 +0.5 611,419 + GFF1283 0.27 -0.8 611,419 + GFF1283 0.27 -0.7 611,419 + GFF1283 0.27 -0.1 611,419 + GFF1283 0.27 -1.5 611,419 + GFF1283 0.27 +0.5 611,419 + GFF1283 0.27 -0.5 611,419 + GFF1283 0.27 -0.2 611,419 + GFF1283 0.27 -0.6 611,420 - GFF1283 0.27 -1.1 611,420 - GFF1283 0.27 -0.5 611,420 - GFF1283 0.27 +0.1 611,420 - GFF1283 0.27 -0.2 611,420 - GFF1283 0.27 -1.5 611,420 - GFF1283 0.27 +0.2 611,464 + GFF1283 0.33 +0.3 611,465 - GFF1283 0.34 -0.1 611,465 - GFF1283 0.34 +1.1 611,465 - GFF1283 0.34 -0.9 611,536 + GFF1283 0.45 -0.2 611,537 - GFF1283 0.45 -1.5 611,537 - GFF1283 0.45 +2.0
Or see this region's nucleotide sequence