Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1233 and GFF1234 overlap by 4 nucleotides GFF1234 and GFF1235 are separated by 102 nucleotides GFF1235 and GFF1236 are separated by 104 nucleotides GFF1236 and GFF1237 are separated by 7 nucleotides
GFF1233 - Arginine/ornithine antiporter ArcD, at 553,231 to 553,875
GFF1233
GFF1234 - Peptide-methionine (S)-S-oxide reductase MsrA (EC 1.8.4.11), at 553,872 to 554,573
GFF1234
GFF1235 - Methionine sulfoxide reductase-associated methionine-rich protein, at 554,676 to 554,942
GFF1235
GFF1236 - Methionine sulfoxide reductase molybdopterin-binding subunit, at 555,047 to 555,838
GFF1236
GFF1237 - Methionine sulfoxide reductase cytochrome b subunit, at 555,846 to 556,502
GFF1237
Position (kb)
554
555 Strain fitness (log2 ratio)
-2
-1
0
1 at 553.915 kb on + strand at 553.915 kb on + strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.975 kb on + strand, within GFF1234 at 553.976 kb on - strand, within GFF1234 at 553.976 kb on - strand, within GFF1234 at 554.002 kb on + strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.015 kb on - strand, within GFF1234 at 554.075 kb on - strand, within GFF1234 at 554.075 kb on - strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.158 kb on + strand, within GFF1234 at 554.158 kb on + strand, within GFF1234 at 554.221 kb on + strand, within GFF1234 at 554.222 kb on - strand, within GFF1234 at 554.242 kb on + strand, within GFF1234 at 554.263 kb on + strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.347 kb on + strand, within GFF1234 at 554.518 kb on + strand at 554.677 kb on + strand at 554.677 kb on + strand at 554.678 kb on - strand at 554.678 kb on - strand at 554.678 kb on - strand at 555.069 kb on + strand at 555.069 kb on + strand at 555.088 kb on - strand at 555.099 kb on + strand at 555.099 kb on + strand at 555.100 kb on - strand at 555.144 kb on + strand, within GFF1236 at 555.145 kb on - strand, within GFF1236 at 555.145 kb on - strand, within GFF1236 at 555.186 kb on + strand, within GFF1236 at 555.186 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.274 kb on - strand, within GFF1236 at 555.274 kb on - strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.490 kb on - strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.529 kb on - strand, within GFF1236 at 555.529 kb on - strand, within GFF1236 at 555.531 kb on + strand, within GFF1236 at 555.532 kb on - strand, within GFF1236 at 555.573 kb on + strand, within GFF1236 at 555.574 kb on - strand, within GFF1236
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 553,915 + -0.6 553,915 + +0.5 553,916 - -1.0 553,916 - +1.0 553,916 - -0.3 553,916 - -0.8 553,916 - -1.5 553,975 + GFF1234 0.15 -0.0 553,976 - GFF1234 0.15 +0.6 553,976 - GFF1234 0.15 -0.8 554,002 + GFF1234 0.19 -0.5 554,003 - GFF1234 0.19 -1.0 554,003 - GFF1234 0.19 -0.5 554,003 - GFF1234 0.19 +0.4 554,015 - GFF1234 0.20 +0.8 554,075 - GFF1234 0.29 -0.4 554,075 - GFF1234 0.29 +1.2 554,104 + GFF1234 0.33 -0.5 554,104 + GFF1234 0.33 -1.3 554,104 + GFF1234 0.33 -0.2 554,105 - GFF1234 0.33 +0.0 554,105 - GFF1234 0.33 +0.1 554,105 - GFF1234 0.33 +0.4 554,105 - GFF1234 0.33 +0.1 554,105 - GFF1234 0.33 -1.2 554,158 + GFF1234 0.41 -0.6 554,158 + GFF1234 0.41 -0.8 554,221 + GFF1234 0.50 +0.7 554,222 - GFF1234 0.50 -0.1 554,242 + GFF1234 0.53 +0.2 554,263 + GFF1234 0.56 +0.0 554,264 - GFF1234 0.56 -1.4 554,264 - GFF1234 0.56 -1.0 554,264 - GFF1234 0.56 +0.3 554,347 + GFF1234 0.68 -0.3 554,518 + +0.9 554,677 + +0.7 554,677 + +0.8 554,678 - -0.7 554,678 - -0.5 554,678 - -0.4 555,069 + -0.2 555,069 + -2.0 555,088 - +0.2 555,099 + -0.5 555,099 + -0.2 555,100 - +0.6 555,144 + GFF1236 0.12 +0.1 555,145 - GFF1236 0.12 -0.5 555,145 - GFF1236 0.12 +0.3 555,186 + GFF1236 0.18 -1.4 555,186 + GFF1236 0.18 +0.1 555,270 + GFF1236 0.28 -0.2 555,270 + GFF1236 0.28 +0.3 555,270 + GFF1236 0.28 -1.6 555,270 + GFF1236 0.28 -0.8 555,270 + GFF1236 0.28 +0.7 555,270 + GFF1236 0.28 -1.8 555,271 - GFF1236 0.28 +0.6 555,271 - GFF1236 0.28 -0.7 555,271 - GFF1236 0.28 -0.1 555,273 + GFF1236 0.29 -1.2 555,273 + GFF1236 0.29 -0.2 555,273 + GFF1236 0.29 +0.6 555,274 - GFF1236 0.29 -0.1 555,274 - GFF1236 0.29 +1.4 555,300 + GFF1236 0.32 -0.1 555,300 + GFF1236 0.32 +0.8 555,300 + GFF1236 0.32 -1.4 555,300 + GFF1236 0.32 -0.4 555,301 - GFF1236 0.32 -0.1 555,301 - GFF1236 0.32 -1.0 555,301 - GFF1236 0.32 +0.9 555,301 - GFF1236 0.32 -0.2 555,489 + GFF1236 0.56 -0.0 555,489 + GFF1236 0.56 +1.1 555,489 + GFF1236 0.56 -1.6 555,490 - GFF1236 0.56 -0.2 555,528 + GFF1236 0.61 -1.1 555,528 + GFF1236 0.61 -0.4 555,528 + GFF1236 0.61 +0.8 555,529 - GFF1236 0.61 -1.9 555,529 - GFF1236 0.61 +0.2 555,531 + GFF1236 0.61 -0.1 555,532 - GFF1236 0.61 -0.3 555,573 + GFF1236 0.66 -0.2 555,574 - GFF1236 0.67 -0.1
Or see this region's nucleotide sequence