Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1186 and GFF1187 are separated by 5 nucleotides GFF1187 and GFF1188 overlap by 4 nucleotides GFF1188 and GFF1189 are separated by 21 nucleotides GFF1189 and GFF1190 overlap by 4 nucleotides
GFF1186 - Ferrichrome-iron receptor, at 502,629 to 504,782
GFF1186
GFF1187 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 504,788 to 505,729
GFF1187
GFF1188 - Thiazole synthase (EC 2.8.1.10), at 505,726 to 506,541
GFF1188
GFF1189 - Sulfur carrier protein ThiS, at 506,563 to 506,760
GFF1189
GFF1190 - Glycine oxidase ThiO (EC 1.4.3.19), at 506,757 to 507,848
GFF1190
Position (kb) 505
506
507 Strain fitness (log2 ratio) -2
-1
0
1 at 504.774 kb on + strand at 504.774 kb on + strand at 504.774 kb on + strand at 504.975 kb on + strand, within GFF1187 at 505.035 kb on + strand, within GFF1187 at 505.037 kb on + strand, within GFF1187 at 505.038 kb on - strand, within GFF1187 at 505.038 kb on - strand, within GFF1187 at 505.038 kb on - strand, within GFF1187 at 505.038 kb on - strand, within GFF1187 at 505.056 kb on + strand, within GFF1187 at 505.056 kb on + strand, within GFF1187 at 505.056 kb on + strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.057 kb on - strand, within GFF1187 at 505.096 kb on - strand, within GFF1187 at 505.096 kb on - strand, within GFF1187 at 505.197 kb on + strand, within GFF1187 at 505.240 kb on - strand, within GFF1187 at 505.392 kb on + strand, within GFF1187 at 505.392 kb on + strand, within GFF1187 at 505.393 kb on - strand, within GFF1187 at 505.602 kb on + strand, within GFF1187 at 505.602 kb on + strand, within GFF1187 at 505.603 kb on - strand, within GFF1187 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.628 kb on - strand, within GFF1189 at 506.998 kb on + strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 507.109 kb on + strand, within GFF1190 at 507.109 kb on + strand, within GFF1190 at 507.110 kb on - strand, within GFF1190
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 504,774 + -0.2 504,774 + -1.9 504,774 + +0.1 504,975 + GFF1187 0.20 -0.3 505,035 + GFF1187 0.26 -0.1 505,037 + GFF1187 0.26 +0.1 505,038 - GFF1187 0.27 +0.4 505,038 - GFF1187 0.27 +0.9 505,038 - GFF1187 0.27 +0.4 505,038 - GFF1187 0.27 +0.4 505,056 + GFF1187 0.28 +0.6 505,056 + GFF1187 0.28 +0.2 505,056 + GFF1187 0.28 -1.6 505,057 - GFF1187 0.29 +1.6 505,057 - GFF1187 0.29 +1.4 505,057 - GFF1187 0.29 -1.6 505,057 - GFF1187 0.29 +0.5 505,057 - GFF1187 0.29 -1.8 505,057 - GFF1187 0.29 +0.6 505,057 - GFF1187 0.29 -0.1 505,057 - GFF1187 0.29 +0.1 505,096 - GFF1187 0.33 -1.4 505,096 - GFF1187 0.33 +0.0 505,197 + GFF1187 0.43 +0.1 505,240 - GFF1187 0.48 -0.2 505,392 + GFF1187 0.64 -0.9 505,392 + GFF1187 0.64 +0.2 505,393 - GFF1187 0.64 +0.4 505,602 + GFF1187 0.86 -0.3 505,602 + GFF1187 0.86 -1.0 505,603 - GFF1187 0.87 +0.1 506,118 - GFF1188 0.48 -0.4 506,118 - GFF1188 0.48 -1.2 506,118 - GFF1188 0.48 -1.0 506,118 - GFF1188 0.48 -0.9 506,196 - GFF1188 0.58 -1.5 506,196 - GFF1188 0.58 +0.4 506,196 - GFF1188 0.58 +0.7 506,628 - GFF1189 0.33 -0.1 506,998 + GFF1190 0.22 -0.1 506,999 - GFF1190 0.22 -0.1 506,999 - GFF1190 0.22 -0.8 506,999 - GFF1190 0.22 -0.5 506,999 - GFF1190 0.22 +0.1 507,109 + GFF1190 0.32 -2.6 507,109 + GFF1190 0.32 +0.7 507,110 - GFF1190 0.32 -0.9
Or see this region's nucleotide sequence