Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1066 and GFF1067 are separated by 11 nucleotides GFF1067 and GFF1068 are separated by 2 nucleotides
GFF1066 - Aspartate aminotransferase (EC 2.6.1.1), at 379,043 to 380,218
GFF1066
GFF1067 - Hydrolase, alpha/beta fold family, at 380,230 to 381,111
GFF1067
GFF1068 - Beta-ureidopropionase (EC 3.5.1.6), at 381,114 to 382,403
GFF1068
Position (kb)
380
381
382 Strain fitness (log2 ratio)
-1
0
1 at 379.238 kb on + strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.539 kb on - strand, within GFF1066 at 379.539 kb on - strand, within GFF1066 at 379.571 kb on - strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.649 kb on + strand, within GFF1066 at 379.650 kb on - strand, within GFF1066 at 379.795 kb on - strand, within GFF1066 at 379.795 kb on - strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.266 kb on + strand at 380.267 kb on - strand at 380.269 kb on + strand at 380.269 kb on + strand at 380.597 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand at 381.265 kb on + strand, within GFF1068 at 381.266 kb on - strand, within GFF1068 at 381.340 kb on + strand, within GFF1068 at 381.341 kb on - strand, within GFF1068 at 381.436 kb on + strand, within GFF1068 at 381.715 kb on - strand, within GFF1068 at 381.728 kb on + strand, within GFF1068 at 381.859 kb on - strand, within GFF1068
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 379,238 + GFF1066 0.17 -0.5 379,239 - GFF1066 0.17 -0.4 379,239 - GFF1066 0.17 +0.2 379,239 - GFF1066 0.17 -0.5 379,239 - GFF1066 0.17 -0.2 379,538 + GFF1066 0.42 +0.5 379,538 + GFF1066 0.42 -0.9 379,538 + GFF1066 0.42 -0.5 379,539 - GFF1066 0.42 +0.7 379,539 - GFF1066 0.42 +0.5 379,571 - GFF1066 0.45 +0.5 379,574 + GFF1066 0.45 +1.2 379,574 + GFF1066 0.45 +1.0 379,574 + GFF1066 0.45 -0.6 379,575 - GFF1066 0.45 +0.7 379,575 - GFF1066 0.45 +1.1 379,575 - GFF1066 0.45 -0.1 379,649 + GFF1066 0.52 -0.4 379,650 - GFF1066 0.52 -0.8 379,795 - GFF1066 0.64 +0.8 379,795 - GFF1066 0.64 +0.2 380,078 + GFF1066 0.88 +0.2 380,078 + GFF1066 0.88 -0.1 380,078 + GFF1066 0.88 -0.0 380,266 + -0.7 380,267 - -0.1 380,269 + -1.1 380,269 + +0.3 380,597 - GFF1067 0.42 +1.0 380,913 - GFF1067 0.77 +0.8 380,913 - GFF1067 0.77 +0.4 381,144 - +0.1 381,144 - -0.2 381,144 - -0.8 381,144 - -0.2 381,265 + GFF1068 0.12 +0.2 381,266 - GFF1068 0.12 -0.6 381,340 + GFF1068 0.18 +0.3 381,341 - GFF1068 0.18 -0.2 381,436 + GFF1068 0.25 +0.2 381,715 - GFF1068 0.47 +0.8 381,728 + GFF1068 0.48 +0.4 381,859 - GFF1068 0.58 -0.1
Or see this region's nucleotide sequence