Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF874 and GFF875 are separated by 174 nucleotides GFF875 and GFF876 are separated by 104 nucleotides GFF876 and GFF877 overlap by 4 nucleotides
GFF874 - no description, at 183,148 to 184,188
GFF874
GFF875 - no description, at 184,363 to 184,743
GFF875
GFF876 - hypothetical protein, at 184,848 to 186,509
GFF876
GFF877 - putative membrane protein, at 186,506 to 187,498
GFF877
Position (kb) 184
185
186
187 Strain fitness (log2 ratio) -2
-1
0
1
2 at 183.850 kb on + strand, within GFF874 at 183.850 kb on + strand, within GFF874 at 183.851 kb on - strand, within GFF874 at 183.851 kb on - strand, within GFF874 at 183.917 kb on - strand, within GFF874 at 184.439 kb on - strand, within GFF875 at 184.439 kb on - strand, within GFF875 at 184.439 kb on - strand, within GFF875 at 184.525 kb on + strand, within GFF875 at 184.526 kb on - strand, within GFF875 at 184.741 kb on + strand at 184.927 kb on + strand at 185.075 kb on - strand, within GFF876 at 185.075 kb on - strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.185 kb on + strand, within GFF876 at 185.186 kb on - strand, within GFF876 at 185.186 kb on - strand, within GFF876 at 185.186 kb on - strand, within GFF876 at 185.186 kb on - strand, within GFF876 at 185.186 kb on - strand, within GFF876 at 185.488 kb on + strand, within GFF876 at 185.488 kb on + strand, within GFF876 at 185.489 kb on - strand, within GFF876 at 185.491 kb on + strand, within GFF876 at 185.491 kb on + strand, within GFF876 at 185.491 kb on + strand, within GFF876 at 185.491 kb on + strand, within GFF876 at 185.492 kb on - strand, within GFF876 at 185.492 kb on - strand, within GFF876 at 185.492 kb on - strand, within GFF876 at 185.492 kb on - strand, within GFF876 at 185.680 kb on + strand, within GFF876 at 185.681 kb on - strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.787 kb on - strand, within GFF877 at 187.011 kb on + strand, within GFF877 at 187.012 kb on - strand, within GFF877 at 187.150 kb on - strand, within GFF877 at 187.161 kb on + strand, within GFF877 at 187.162 kb on - strand, within GFF877 at 187.162 kb on - strand, within GFF877 at 187.388 kb on + strand, within GFF877 at 187.476 kb on + strand at 187.476 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 183,850 + GFF874 0.67 +1.2 183,850 + GFF874 0.67 +0.2 183,851 - GFF874 0.68 -0.1 183,851 - GFF874 0.68 +0.7 183,917 - GFF874 0.74 -0.1 184,439 - GFF875 0.20 -1.1 184,439 - GFF875 0.20 -0.5 184,439 - GFF875 0.20 -1.2 184,525 + GFF875 0.43 +1.7 184,526 - GFF875 0.43 -0.3 184,741 + +1.8 184,927 + -0.8 185,075 - GFF876 0.14 -1.3 185,075 - GFF876 0.14 +0.1 185,185 + GFF876 0.20 +2.5 185,185 + GFF876 0.20 -2.4 185,185 + GFF876 0.20 +1.0 185,185 + GFF876 0.20 -0.8 185,185 + GFF876 0.20 -1.1 185,185 + GFF876 0.20 -0.5 185,186 - GFF876 0.20 -0.6 185,186 - GFF876 0.20 +0.1 185,186 - GFF876 0.20 +0.2 185,186 - GFF876 0.20 -1.7 185,186 - GFF876 0.20 +0.4 185,488 + GFF876 0.39 +0.4 185,488 + GFF876 0.39 -0.2 185,489 - GFF876 0.39 -0.5 185,491 + GFF876 0.39 +0.5 185,491 + GFF876 0.39 -0.1 185,491 + GFF876 0.39 +2.0 185,491 + GFF876 0.39 +0.2 185,492 - GFF876 0.39 +0.1 185,492 - GFF876 0.39 -2.0 185,492 - GFF876 0.39 -0.2 185,492 - GFF876 0.39 -1.0 185,680 + GFF876 0.50 +0.3 185,681 - GFF876 0.50 -0.7 186,307 + GFF876 0.88 +1.0 186,307 + GFF876 0.88 -0.1 186,307 + GFF876 0.88 -0.2 186,308 - GFF876 0.88 +0.6 186,308 - GFF876 0.88 -0.3 186,308 - GFF876 0.88 -0.3 186,308 - GFF876 0.88 +0.7 186,308 - GFF876 0.88 +0.6 186,308 - GFF876 0.88 -0.6 186,787 - GFF877 0.28 -0.9 187,011 + GFF877 0.51 -0.4 187,012 - GFF877 0.51 -0.0 187,150 - GFF877 0.65 -0.7 187,161 + GFF877 0.66 -0.1 187,162 - GFF877 0.66 -0.3 187,162 - GFF877 0.66 -0.5 187,388 + GFF877 0.89 +0.2 187,476 + +0.5 187,476 + -0.6
Or see this region's nucleotide sequence