Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF823 and GFF824 are separated by 5 nucleotides GFF824 and GFF825 are separated by 164 nucleotides GFF825 and GFF826 are separated by 173 nucleotides GFF826 and GFF827 are separated by 200 nucleotides
GFF823 - Inner membrane protein YbaL, KefB/KefC family, at 128,145 to 129,851
GFF823
GFF824 - hypothetical protein, at 129,857 to 130,261
GFF824
GFF825 - no description, at 130,426 to 130,653
GFF825
GFF826 - no description, at 130,827 to 130,979
GFF826
GFF827 - Predicted cobalt transporter CbtC, at 131,180 to 131,857
GFF827
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.447 kb on + strand, within GFF823 at 129.448 kb on - strand, within GFF823 at 129.501 kb on + strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.976 kb on + strand, within GFF824 at 129.976 kb on + strand, within GFF824 at 129.976 kb on + strand, within GFF824 at 129.977 kb on - strand, within GFF824 at 129.977 kb on - strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.206 kb on + strand, within GFF824 at 130.207 kb on - strand, within GFF824 at 130.207 kb on - strand, within GFF824 at 130.319 kb on + strand at 130.319 kb on + strand at 130.319 kb on + strand at 130.319 kb on + strand at 130.320 kb on - strand at 130.320 kb on - strand at 130.320 kb on - strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.416 kb on - strand at 130.416 kb on - strand at 130.483 kb on + strand, within GFF825 at 130.483 kb on + strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.567 kb on + strand, within GFF825 at 130.576 kb on + strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.649 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.785 kb on - strand at 130.785 kb on - strand at 130.952 kb on + strand, within GFF826 at 131.078 kb on + strand at 131.078 kb on + strand at 131.078 kb on + strand at 131.079 kb on - strand at 131.079 kb on - strand at 131.174 kb on + strand at 131.174 kb on + strand at 131.174 kb on + strand at 131.174 kb on + strand at 131.175 kb on - strand at 131.175 kb on - strand at 131.175 kb on - strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.228 kb on + strand at 131.229 kb on - strand at 131.229 kb on - strand at 131.229 kb on - strand at 131.229 kb on - strand at 131.255 kb on + strand, within GFF827 at 131.255 kb on + strand, within GFF827 at 131.255 kb on + strand, within GFF827 at 131.255 kb on + strand, within GFF827 at 131.255 kb on + strand, within GFF827 at 131.256 kb on - strand, within GFF827 at 131.256 kb on - strand, within GFF827 at 131.256 kb on - strand, within GFF827
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 129,417 + GFF823 0.75 -0.5 129,417 + GFF823 0.75 +0.1 129,417 + GFF823 0.75 +1.7 129,417 + GFF823 0.75 -0.2 129,417 + GFF823 0.75 -0.2 129,417 + GFF823 0.75 -0.5 129,417 + GFF823 0.75 -0.1 129,417 + GFF823 0.75 -0.4 129,418 - GFF823 0.75 -1.0 129,418 - GFF823 0.75 -0.2 129,418 - GFF823 0.75 +1.4 129,418 - GFF823 0.75 +0.8 129,418 - GFF823 0.75 +1.0 129,418 - GFF823 0.75 -0.8 129,418 - GFF823 0.75 -1.0 129,447 + GFF823 0.76 -0.7 129,448 - GFF823 0.76 +0.1 129,501 + GFF823 0.79 -0.2 129,502 - GFF823 0.79 +0.3 129,502 - GFF823 0.79 -1.3 129,502 - GFF823 0.79 -0.9 129,502 - GFF823 0.79 -0.6 129,976 + GFF824 0.29 +1.0 129,976 + GFF824 0.29 +0.6 129,976 + GFF824 0.29 -0.8 129,977 - GFF824 0.30 -0.3 129,977 - GFF824 0.30 -1.4 130,002 + GFF824 0.36 -0.5 130,002 + GFF824 0.36 -0.3 130,002 + GFF824 0.36 +0.6 130,002 + GFF824 0.36 +0.4 130,003 - GFF824 0.36 -0.1 130,003 - GFF824 0.36 -0.6 130,003 - GFF824 0.36 +1.7 130,107 + GFF824 0.62 -0.5 130,107 + GFF824 0.62 +0.4 130,107 + GFF824 0.62 -0.2 130,107 + GFF824 0.62 +0.3 130,108 - GFF824 0.62 -1.1 130,108 - GFF824 0.62 +0.5 130,108 - GFF824 0.62 -0.8 130,108 - GFF824 0.62 +0.5 130,206 + GFF824 0.86 +1.3 130,207 - GFF824 0.86 +0.5 130,207 - GFF824 0.86 +0.0 130,319 + -1.5 130,319 + -0.8 130,319 + +0.8 130,319 + -0.1 130,320 - +0.7 130,320 - -1.2 130,320 - -0.5 130,327 + +0.8 130,327 + -1.7 130,327 + +2.1 130,327 + -1.3 130,327 + -0.8 130,327 + -0.8 130,327 + +1.0 130,327 + -0.7 130,327 + -0.3 130,327 + -0.2 130,327 + -0.2 130,327 + +0.1 130,327 + +0.5 130,328 - -0.3 130,328 - -0.3 130,328 - -0.5 130,328 - +0.5 130,328 - -0.3 130,328 - -0.8 130,328 - -0.6 130,328 - -0.7 130,328 - -0.3 130,328 - -0.5 130,328 - -0.1 130,328 - -0.5 130,328 - -0.2 130,353 - +0.2 130,353 - +0.8 130,353 - -0.9 130,353 - +0.2 130,353 - +0.2 130,416 - +1.2 130,416 - -0.5 130,483 + GFF825 0.25 -1.0 130,483 + GFF825 0.25 -0.1 130,484 - GFF825 0.25 +0.3 130,484 - GFF825 0.25 -0.9 130,484 - GFF825 0.25 -0.3 130,484 - GFF825 0.25 -0.4 130,484 - GFF825 0.25 +0.5 130,537 + GFF825 0.49 +0.3 130,537 + GFF825 0.49 +0.1 130,537 + GFF825 0.49 +0.9 130,537 + GFF825 0.49 +0.1 130,537 + GFF825 0.49 +0.7 130,537 + GFF825 0.49 +0.1 130,538 - GFF825 0.49 -0.8 130,538 - GFF825 0.49 -1.0 130,538 - GFF825 0.49 +0.1 130,538 - GFF825 0.49 +0.6 130,538 - GFF825 0.49 -0.2 130,538 - GFF825 0.49 -1.2 130,538 - GFF825 0.49 +1.1 130,538 - GFF825 0.49 -0.8 130,538 - GFF825 0.49 +0.3 130,567 + GFF825 0.62 +1.1 130,576 + GFF825 0.66 -0.8 130,577 - GFF825 0.66 -0.1 130,577 - GFF825 0.66 -1.2 130,577 - GFF825 0.66 -0.4 130,649 + -2.3 130,651 + -0.2 130,651 + -0.8 130,651 + -1.7 130,651 + -1.1 130,652 - -0.9 130,652 - -0.3 130,652 - -0.5 130,652 - -0.1 130,652 - -0.4 130,652 - -0.1 130,652 - -0.1 130,784 + +0.7 130,784 + -0.6 130,784 + +0.3 130,784 + -0.4 130,785 - +0.3 130,785 - +0.7 130,952 + GFF826 0.82 -2.3 131,078 + -1.6 131,078 + +0.2 131,078 + -0.4 131,079 - -0.1 131,079 - +0.2 131,174 + -0.7 131,174 + -0.6 131,174 + +0.6 131,174 + +1.5 131,175 - +1.5 131,175 - -0.5 131,175 - +0.9 131,228 + +1.5 131,228 + -0.1 131,228 + -0.8 131,228 + -1.3 131,228 + +0.6 131,228 + -0.1 131,228 + -0.3 131,229 - +1.8 131,229 - -0.5 131,229 - -0.4 131,229 - +0.0 131,255 + GFF827 0.11 +0.5 131,255 + GFF827 0.11 +0.5 131,255 + GFF827 0.11 -0.6 131,255 + GFF827 0.11 -1.2 131,255 + GFF827 0.11 -0.2 131,256 - GFF827 0.11 +3.1 131,256 - GFF827 0.11 -0.6 131,256 - GFF827 0.11 -0.2
Or see this region's nucleotide sequence