Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7385 and GFF7386 overlap by 4 nucleotides GFF7386 and GFF7387 are separated by 33 nucleotides GFF7387 and GFF7388 are separated by 6 nucleotides GFF7388 and GFF7389 are separated by 107 nucleotides
GFF7385 - hypothetical protein, at 3,093 to 4,460
GFF7385
GFF7386 - no description, at 4,457 to 5,329
GFF7386
GFF7387 - no description, at 5,363 to 5,500
GFF7387
GFF7388 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 5,507 to 6,259
GFF7388
GFF7389 - Cyclohexanone monooxygenase (EC 1.14.13.22), at 6,367 to 7,797
GFF7389
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.667 kb on + strand, within GFF7386 at 4.667 kb on + strand, within GFF7386 at 4.667 kb on + strand, within GFF7386 at 4.717 kb on - strand, within GFF7386 at 4.889 kb on + strand, within GFF7386 at 4.944 kb on - strand, within GFF7386 at 4.944 kb on - strand, within GFF7386 at 5.156 kb on + strand, within GFF7386 at 5.156 kb on + strand, within GFF7386 at 5.157 kb on - strand, within GFF7386 at 5.476 kb on + strand, within GFF7387 at 5.498 kb on + strand at 5.498 kb on + strand at 5.499 kb on - strand at 5.712 kb on + strand, within GFF7388 at 5.712 kb on + strand, within GFF7388 at 5.822 kb on + strand, within GFF7388 at 5.888 kb on - strand, within GFF7388 at 5.960 kb on + strand, within GFF7388 at 6.061 kb on - strand, within GFF7388
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 4,667 + GFF7386 0.24 +1.4 4,667 + GFF7386 0.24 -0.1 4,667 + GFF7386 0.24 +0.0 4,717 - GFF7386 0.30 -2.1 4,889 + GFF7386 0.49 -1.1 4,944 - GFF7386 0.56 -0.7 4,944 - GFF7386 0.56 -0.4 5,156 + GFF7386 0.80 -0.7 5,156 + GFF7386 0.80 +1.3 5,157 - GFF7386 0.80 -0.4 5,476 + GFF7387 0.82 +2.2 5,498 + -1.0 5,498 + +0.6 5,499 - -0.5 5,712 + GFF7388 0.27 +0.8 5,712 + GFF7388 0.27 +0.1 5,822 + GFF7388 0.42 -0.4 5,888 - GFF7388 0.51 +0.3 5,960 + GFF7388 0.60 +0.4 6,061 - GFF7388 0.74 +1.2
Or see this region's nucleotide sequence