Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7342 and GFF7343 are separated by 240 nucleotides GFF7343 and GFF7344 are separated by 54 nucleotides GFF7344 and GFF7345 are separated by 137 nucleotides GFF7345 and GFF7346 overlap by 4 nucleotides
GFF7342 - Oxidoreductase, at 10,204 to 11,664
GFF7342
GFF7343 - hypothetical protein, at 11,905 to 12,177
GFF7343
GFF7344 - Transcriptional regulator, LysR family, at 12,232 to 13,221
GFF7344
GFF7345 - Periplasmic aromatic aldehyde oxidoreductase, iron-sulfur subunit YagT, at 13,359 to 14,015
GFF7345
GFF7346 - Periplasmic aromatic aldehyde oxidoreductase, FAD binding subunit YagS, at 14,012 to 14,962
GFF7346
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1 at 11.443 kb on + strand, within GFF7342 at 11.443 kb on + strand, within GFF7342 at 11.443 kb on + strand, within GFF7342 at 11.444 kb on - strand, within GFF7342 at 11.617 kb on + strand at 11.617 kb on + strand at 11.741 kb on + strand at 11.742 kb on - strand at 11.856 kb on + strand at 12.556 kb on - strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.606 kb on - strand, within GFF7344 at 12.721 kb on + strand, within GFF7344 at 12.722 kb on - strand, within GFF7344 at 13.354 kb on + strand at 13.354 kb on + strand at 13.354 kb on + strand at 13.355 kb on - strand at 13.876 kb on - strand, within GFF7345 at 13.953 kb on + strand at 13.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 11,443 + GFF7342 0.85 +0.6 11,443 + GFF7342 0.85 -0.4 11,443 + GFF7342 0.85 +0.6 11,444 - GFF7342 0.85 -0.4 11,617 + -1.6 11,617 + -0.6 11,741 + -0.1 11,742 - -0.8 11,856 + +0.3 12,556 - GFF7344 0.33 +0.1 12,605 + GFF7344 0.38 +0.4 12,605 + GFF7344 0.38 -1.3 12,606 - GFF7344 0.38 -1.0 12,721 + GFF7344 0.49 -0.7 12,722 - GFF7344 0.49 -0.2 13,354 + +0.0 13,354 + +0.3 13,354 + -1.2 13,355 - -0.5 13,876 - GFF7345 0.79 -0.6 13,953 + +0.4 13,953 + -1.0
Or see this region's nucleotide sequence