Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF693 and GFF694 are separated by 18 nucleotides GFF694 and GFF695 overlap by 4 nucleotides GFF695 and GFF696 are separated by 164 nucleotides
GFF693 - BUG/TctC family periplasmic protein, at 748,310 to 749,326
GFF693
GFF694 - 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-), at 749,345 to 750,208
GFF694
GFF695 - Cytochrome B561, at 750,205 to 750,777
GFF695
GFF696 - Glycosyl transferase, group 2 family, at 750,942 to 752,372
GFF696
Position (kb)
750
751 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 749.216 kb on + strand, within GFF693 at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.270 kb on + strand at 749.271 kb on - strand at 749.271 kb on - strand at 749.271 kb on - strand at 749.271 kb on - strand at 749.271 kb on - strand at 749.394 kb on + strand at 749.394 kb on + strand at 749.394 kb on + strand at 749.395 kb on - strand at 749.395 kb on - strand at 749.748 kb on - strand, within GFF694 at 750.302 kb on + strand, within GFF695 at 750.302 kb on + strand, within GFF695 at 750.302 kb on + strand, within GFF695 at 750.303 kb on - strand, within GFF695 at 750.303 kb on - strand, within GFF695 at 750.311 kb on + strand, within GFF695 at 750.311 kb on + strand, within GFF695 at 750.311 kb on + strand, within GFF695 at 750.311 kb on + strand, within GFF695 at 750.311 kb on + strand, within GFF695 at 750.312 kb on - strand, within GFF695 at 750.312 kb on - strand, within GFF695 at 750.312 kb on - strand, within GFF695 at 750.312 kb on - strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.422 kb on + strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.423 kb on - strand, within GFF695 at 750.500 kb on + strand, within GFF695 at 750.500 kb on + strand, within GFF695 at 750.501 kb on - strand, within GFF695 at 750.501 kb on - strand, within GFF695 at 750.501 kb on - strand, within GFF695 at 750.548 kb on + strand, within GFF695 at 750.548 kb on + strand, within GFF695 at 750.548 kb on + strand, within GFF695 at 750.549 kb on - strand, within GFF695 at 750.549 kb on - strand, within GFF695 at 750.675 kb on - strand, within GFF695 at 750.758 kb on + strand at 750.758 kb on + strand at 750.759 kb on - strand at 750.759 kb on - strand at 750.759 kb on - strand at 750.876 kb on + strand at 750.876 kb on + strand at 750.877 kb on - strand at 750.877 kb on - strand at 750.877 kb on - strand at 750.877 kb on - strand at 750.877 kb on - strand at 750.877 kb on - strand at 750.877 kb on - strand at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.086 kb on + strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.087 kb on - strand, within GFF696 at 751.095 kb on + strand, within GFF696 at 751.095 kb on + strand, within GFF696 at 751.095 kb on + strand, within GFF696 at 751.095 kb on + strand, within GFF696 at 751.096 kb on - strand, within GFF696 at 751.096 kb on - strand, within GFF696 at 751.096 kb on - strand, within GFF696 at 751.096 kb on - strand, within GFF696 at 751.234 kb on + strand, within GFF696 at 751.235 kb on - strand, within GFF696 at 751.235 kb on - strand, within GFF696 at 751.405 kb on - strand, within GFF696 at 751.405 kb on - strand, within GFF696 at 751.638 kb on + strand, within GFF696 at 751.638 kb on + strand, within GFF696 at 751.638 kb on + strand, within GFF696 at 751.638 kb on + strand, within GFF696 at 751.639 kb on - strand, within GFF696 at 751.639 kb on - strand, within GFF696 at 751.639 kb on - strand, within GFF696 at 751.639 kb on - strand, within GFF696 at 751.639 kb on - strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.644 kb on + strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696 at 751.645 kb on - strand, within GFF696
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 749,216 + GFF693 0.89 -0.6 749,270 + +0.3 749,270 + +2.0 749,270 + -0.8 749,270 + -0.3 749,270 + -0.5 749,270 + +0.6 749,270 + -0.0 749,270 + +0.2 749,270 + -0.3 749,271 - -0.1 749,271 - -0.0 749,271 - -1.5 749,271 - -0.5 749,271 - +0.4 749,394 + -0.8 749,394 + -1.1 749,394 + -0.4 749,395 - -0.5 749,395 - -1.9 749,748 - GFF694 0.47 +0.1 750,302 + GFF695 0.17 +0.2 750,302 + GFF695 0.17 -2.4 750,302 + GFF695 0.17 -0.7 750,303 - GFF695 0.17 -1.1 750,303 - GFF695 0.17 -2.0 750,311 + GFF695 0.18 -0.6 750,311 + GFF695 0.18 +0.9 750,311 + GFF695 0.18 -1.3 750,311 + GFF695 0.18 -1.0 750,311 + GFF695 0.18 -0.8 750,312 - GFF695 0.19 +0.3 750,312 - GFF695 0.19 -1.8 750,312 - GFF695 0.19 -1.0 750,312 - GFF695 0.19 -0.5 750,422 + GFF695 0.38 +0.1 750,422 + GFF695 0.38 -1.0 750,422 + GFF695 0.38 -0.5 750,422 + GFF695 0.38 -0.4 750,422 + GFF695 0.38 -0.8 750,422 + GFF695 0.38 -1.7 750,422 + GFF695 0.38 +0.2 750,423 - GFF695 0.38 +0.3 750,423 - GFF695 0.38 +1.3 750,423 - GFF695 0.38 +1.1 750,423 - GFF695 0.38 +0.1 750,423 - GFF695 0.38 +1.3 750,423 - GFF695 0.38 +0.5 750,423 - GFF695 0.38 -1.3 750,423 - GFF695 0.38 -2.1 750,423 - GFF695 0.38 +0.7 750,423 - GFF695 0.38 -1.1 750,423 - GFF695 0.38 +0.3 750,423 - GFF695 0.38 -0.1 750,423 - GFF695 0.38 -0.6 750,500 + GFF695 0.51 +0.6 750,500 + GFF695 0.51 -0.3 750,501 - GFF695 0.52 -0.4 750,501 - GFF695 0.52 -0.1 750,501 - GFF695 0.52 +0.5 750,548 + GFF695 0.60 -1.3 750,548 + GFF695 0.60 -1.1 750,548 + GFF695 0.60 -0.5 750,549 - GFF695 0.60 -0.7 750,549 - GFF695 0.60 -0.4 750,675 - GFF695 0.82 -0.5 750,758 + -1.3 750,758 + -1.3 750,759 - -1.3 750,759 - -0.4 750,759 - +0.1 750,876 + -1.4 750,876 + -0.5 750,877 - -0.5 750,877 - +0.1 750,877 - -1.4 750,877 - +0.5 750,877 - +0.2 750,877 - +0.3 750,877 - -1.8 751,086 + GFF696 0.10 -0.1 751,086 + GFF696 0.10 -0.1 751,086 + GFF696 0.10 +0.4 751,086 + GFF696 0.10 +0.7 751,086 + GFF696 0.10 +1.2 751,086 + GFF696 0.10 -0.0 751,086 + GFF696 0.10 -0.1 751,086 + GFF696 0.10 +0.0 751,086 + GFF696 0.10 -1.0 751,086 + GFF696 0.10 -0.8 751,086 + GFF696 0.10 +0.3 751,086 + GFF696 0.10 -1.5 751,086 + GFF696 0.10 +0.6 751,087 - GFF696 0.10 -0.8 751,087 - GFF696 0.10 +0.3 751,087 - GFF696 0.10 -0.3 751,087 - GFF696 0.10 -0.1 751,087 - GFF696 0.10 -0.8 751,087 - GFF696 0.10 +0.2 751,087 - GFF696 0.10 -1.7 751,087 - GFF696 0.10 -0.1 751,087 - GFF696 0.10 -0.7 751,087 - GFF696 0.10 +1.6 751,087 - GFF696 0.10 +1.3 751,095 + GFF696 0.11 -0.3 751,095 + GFF696 0.11 -0.1 751,095 + GFF696 0.11 +1.3 751,095 + GFF696 0.11 -0.3 751,096 - GFF696 0.11 -1.3 751,096 - GFF696 0.11 -0.2 751,096 - GFF696 0.11 -0.0 751,096 - GFF696 0.11 -0.3 751,234 + GFF696 0.20 -0.0 751,235 - GFF696 0.20 +0.3 751,235 - GFF696 0.20 -0.7 751,405 - GFF696 0.32 -0.1 751,405 - GFF696 0.32 -0.3 751,638 + GFF696 0.49 -0.3 751,638 + GFF696 0.49 +0.2 751,638 + GFF696 0.49 -0.0 751,638 + GFF696 0.49 -0.8 751,639 - GFF696 0.49 -0.3 751,639 - GFF696 0.49 +0.3 751,639 - GFF696 0.49 +1.6 751,639 - GFF696 0.49 -0.7 751,639 - GFF696 0.49 -0.2 751,644 + GFF696 0.49 -0.1 751,644 + GFF696 0.49 +0.2 751,644 + GFF696 0.49 -0.6 751,644 + GFF696 0.49 -0.8 751,644 + GFF696 0.49 -1.6 751,644 + GFF696 0.49 -0.5 751,644 + GFF696 0.49 -0.1 751,644 + GFF696 0.49 -0.6 751,644 + GFF696 0.49 -0.5 751,644 + GFF696 0.49 -2.8 751,644 + GFF696 0.49 -0.2 751,645 - GFF696 0.49 -0.1 751,645 - GFF696 0.49 -0.2 751,645 - GFF696 0.49 -0.6 751,645 - GFF696 0.49 -1.0 751,645 - GFF696 0.49 -0.1 751,645 - GFF696 0.49 -0.2 751,645 - GFF696 0.49 +1.3 751,645 - GFF696 0.49 -0.1 751,645 - GFF696 0.49 -0.0 751,645 - GFF696 0.49 -0.4 751,645 - GFF696 0.49 +0.4
Or see this region's nucleotide sequence