Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6794 and GFF6795 overlap by 4 nucleotides GFF6795 and GFF6796 are separated by 19 nucleotides GFF6796 and GFF6797 are separated by 194 nucleotides GFF6797 and GFF6798 overlap by 17 nucleotides
GFF6794 - hypothetical protein, at 42,198 to 43,739
GFF6794
GFF6795 - similar to puromycin N-acetyltransferase, at 43,736 to 44,224
GFF6795
GFF6796 - Predicted kinase, at 44,244 to 44,750
GFF6796
GFF6797 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 44,945 to 45,730
GFF6797
GFF6798 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 45,714 to 46,367
GFF6798
Position (kb)
44
45 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 43.615 kb on - strand at 43.615 kb on - strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.734 kb on + strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.735 kb on - strand at 43.737 kb on + strand at 43.738 kb on - strand at 43.758 kb on + strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.759 kb on - strand at 43.821 kb on + strand, within GFF6795 at 43.821 kb on + strand, within GFF6795 at 43.821 kb on + strand, within GFF6795 at 43.822 kb on - strand, within GFF6795 at 43.822 kb on - strand, within GFF6795 at 44.070 kb on + strand, within GFF6795 at 44.237 kb on + strand at 44.238 kb on - strand at 44.395 kb on - strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.569 kb on + strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.570 kb on - strand, within GFF6796 at 44.600 kb on - strand, within GFF6796 at 44.712 kb on + strand at 44.712 kb on + strand at 44.712 kb on + strand at 44.713 kb on - strand at 44.713 kb on - strand at 44.854 kb on - strand at 44.919 kb on + strand at 45.643 kb on - strand, within GFF6797 at 45.643 kb on - strand, within GFF6797
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 43,615 - -0.3 43,615 - -1.0 43,734 + -1.1 43,734 + +1.4 43,734 + +0.9 43,734 + -0.1 43,735 - +1.3 43,735 - -0.1 43,735 - -0.4 43,735 - -0.3 43,735 - -0.8 43,735 - -0.3 43,735 - -0.3 43,737 + -2.1 43,738 - -1.0 43,758 + -0.3 43,759 - -0.1 43,759 - -1.1 43,759 - -0.1 43,759 - +0.1 43,759 - +0.3 43,821 + GFF6795 0.17 -0.3 43,821 + GFF6795 0.17 -3.1 43,821 + GFF6795 0.17 +3.1 43,822 - GFF6795 0.18 +0.5 43,822 - GFF6795 0.18 -0.6 44,070 + GFF6795 0.68 -0.3 44,237 + +0.7 44,238 - +0.7 44,395 - GFF6796 0.30 -0.2 44,569 + GFF6796 0.64 -0.2 44,569 + GFF6796 0.64 -0.5 44,569 + GFF6796 0.64 -2.1 44,569 + GFF6796 0.64 +0.6 44,569 + GFF6796 0.64 -0.1 44,569 + GFF6796 0.64 -0.8 44,570 - GFF6796 0.64 -0.5 44,570 - GFF6796 0.64 +1.1 44,570 - GFF6796 0.64 -1.0 44,570 - GFF6796 0.64 -0.1 44,570 - GFF6796 0.64 -1.0 44,570 - GFF6796 0.64 -0.9 44,600 - GFF6796 0.70 +0.1 44,712 + +1.8 44,712 + -0.1 44,712 + -1.0 44,713 - -0.6 44,713 - -0.7 44,854 - -0.8 44,919 + -0.9 45,643 - GFF6797 0.89 -0.3 45,643 - GFF6797 0.89 +0.8
Or see this region's nucleotide sequence