Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6555 and GFF6556 are separated by 100 nucleotides GFF6556 and GFF6557 are separated by 129 nucleotides GFF6557 and GFF6558 are separated by 84 nucleotides
GFF6555 - no description, at 51,524 to 51,907
GFF6555
GFF6556 - hypothetical protein, at 52,008 to 52,367
GFF6556
GFF6557 - hypothetical protein, at 52,497 to 53,798
GFF6557
GFF6558 - 5'-nucleotidase (EC 3.1.3.5), at 53,883 to 55,712
GFF6558
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.531 kb on + strand at 51.866 kb on + strand, within GFF6555 at 51.992 kb on - strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.994 kb on + strand at 51.995 kb on - strand at 51.995 kb on - strand at 51.995 kb on - strand at 51.995 kb on - strand at 51.995 kb on - strand at 52.016 kb on + strand at 52.016 kb on + strand at 52.016 kb on + strand at 52.017 kb on - strand at 52.017 kb on - strand at 52.239 kb on + strand, within GFF6556 at 52.240 kb on - strand, within GFF6556 at 52.240 kb on - strand, within GFF6556 at 52.277 kb on - strand, within GFF6556 at 52.284 kb on + strand, within GFF6556 at 52.285 kb on - strand, within GFF6556 at 52.285 kb on - strand, within GFF6556 at 52.420 kb on + strand at 52.420 kb on + strand at 52.420 kb on + strand at 52.527 kb on + strand at 52.527 kb on + strand at 52.590 kb on + strand at 52.590 kb on + strand at 52.591 kb on - strand at 52.591 kb on - strand at 52.591 kb on - strand at 52.591 kb on - strand at 52.698 kb on + strand, within GFF6557 at 52.882 kb on - strand, within GFF6557 at 52.929 kb on + strand, within GFF6557 at 52.929 kb on + strand, within GFF6557 at 52.930 kb on - strand, within GFF6557 at 52.930 kb on - strand, within GFF6557 at 52.962 kb on + strand, within GFF6557 at 52.962 kb on + strand, within GFF6557 at 52.962 kb on + strand, within GFF6557 at 52.962 kb on + strand, within GFF6557 at 52.963 kb on - strand, within GFF6557 at 53.272 kb on + strand, within GFF6557 at 53.272 kb on + strand, within GFF6557 at 53.373 kb on + strand, within GFF6557 at 53.373 kb on + strand, within GFF6557 at 53.373 kb on + strand, within GFF6557 at 53.373 kb on + strand, within GFF6557 at 53.374 kb on - strand, within GFF6557 at 53.374 kb on - strand, within GFF6557 at 53.399 kb on - strand, within GFF6557 at 53.475 kb on + strand, within GFF6557 at 53.475 kb on + strand, within GFF6557 at 53.475 kb on + strand, within GFF6557 at 53.475 kb on + strand, within GFF6557 at 53.476 kb on - strand, within GFF6557 at 53.568 kb on + strand, within GFF6557 at 53.568 kb on + strand, within GFF6557 at 53.568 kb on + strand, within GFF6557 at 53.568 kb on + strand, within GFF6557 at 53.569 kb on - strand, within GFF6557 at 53.757 kb on + strand at 53.758 kb on - strand at 53.859 kb on + strand at 53.860 kb on - strand at 53.860 kb on - strand at 53.863 kb on + strand at 53.863 kb on + strand at 53.904 kb on - strand at 53.950 kb on + strand at 53.950 kb on + strand at 53.950 kb on + strand at 53.951 kb on - strand at 53.951 kb on - strand at 53.951 kb on - strand at 53.951 kb on - strand at 54.061 kb on + strand at 54.061 kb on + strand at 54.061 kb on + strand at 54.061 kb on + strand at 54.062 kb on - strand at 54.062 kb on - strand at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.139 kb on + strand, within GFF6558 at 54.140 kb on - strand, within GFF6558 at 54.140 kb on - strand, within GFF6558 at 54.140 kb on - strand, within GFF6558 at 54.140 kb on - strand, within GFF6558 at 54.145 kb on + strand, within GFF6558 at 54.145 kb on + strand, within GFF6558 at 54.230 kb on + strand, within GFF6558 at 54.230 kb on + strand, within GFF6558 at 54.258 kb on + strand, within GFF6558 at 54.301 kb on + strand, within GFF6558 at 54.302 kb on - strand, within GFF6558 at 54.526 kb on + strand, within GFF6558 at 54.526 kb on + strand, within GFF6558 at 54.526 kb on + strand, within GFF6558 at 54.526 kb on + strand, within GFF6558 at 54.527 kb on - strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.592 kb on + strand, within GFF6558 at 54.593 kb on - strand, within GFF6558 at 54.593 kb on - strand, within GFF6558 at 54.593 kb on - strand, within GFF6558 at 54.613 kb on + strand, within GFF6558 at 54.613 kb on + strand, within GFF6558 at 54.613 kb on + strand, within GFF6558 at 54.614 kb on - strand, within GFF6558 at 54.614 kb on - strand, within GFF6558 at 54.614 kb on - strand, within GFF6558 at 54.712 kb on + strand, within GFF6558 at 54.764 kb on - strand, within GFF6558 at 54.775 kb on + strand, within GFF6558 at 54.775 kb on + strand, within GFF6558 at 54.775 kb on + strand, within GFF6558 at 54.775 kb on + strand, within GFF6558 at 54.775 kb on + strand, within GFF6558 at 54.776 kb on - strand, within GFF6558 at 54.776 kb on - strand, within GFF6558 at 54.776 kb on - strand, within GFF6558 at 54.776 kb on - strand, within GFF6558
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 51,531 + -0.3 51,866 + GFF6555 0.89 +0.1 51,992 - -0.2 51,994 + -1.6 51,994 + +0.9 51,994 + -0.7 51,994 + -2.0 51,994 + +0.5 51,994 + -0.6 51,994 + +1.2 51,994 + -0.6 51,994 + +0.8 51,994 + -0.2 51,995 - +0.1 51,995 - -0.5 51,995 - +0.4 51,995 - -0.7 51,995 - -0.0 52,016 + -0.2 52,016 + -0.2 52,016 + +0.9 52,017 - -1.3 52,017 - +0.1 52,239 + GFF6556 0.64 +0.5 52,240 - GFF6556 0.64 -1.0 52,240 - GFF6556 0.64 +0.3 52,277 - GFF6556 0.75 -0.6 52,284 + GFF6556 0.77 -0.5 52,285 - GFF6556 0.77 -0.6 52,285 - GFF6556 0.77 -0.1 52,420 + -0.5 52,420 + +0.2 52,420 + -1.0 52,527 + +0.4 52,527 + +0.2 52,590 + +0.3 52,590 + +0.6 52,591 - -0.3 52,591 - -2.5 52,591 - -0.1 52,591 - -0.3 52,698 + GFF6557 0.15 -1.1 52,882 - GFF6557 0.30 -0.3 52,929 + GFF6557 0.33 -1.1 52,929 + GFF6557 0.33 +0.2 52,930 - GFF6557 0.33 +0.4 52,930 - GFF6557 0.33 -0.2 52,962 + GFF6557 0.36 +0.3 52,962 + GFF6557 0.36 +1.1 52,962 + GFF6557 0.36 +0.6 52,962 + GFF6557 0.36 +0.3 52,963 - GFF6557 0.36 -0.4 53,272 + GFF6557 0.60 +0.1 53,272 + GFF6557 0.60 +0.6 53,373 + GFF6557 0.67 -0.1 53,373 + GFF6557 0.67 -0.8 53,373 + GFF6557 0.67 +0.5 53,373 + GFF6557 0.67 -1.4 53,374 - GFF6557 0.67 -0.1 53,374 - GFF6557 0.67 +0.8 53,399 - GFF6557 0.69 +0.2 53,475 + GFF6557 0.75 +1.1 53,475 + GFF6557 0.75 -0.8 53,475 + GFF6557 0.75 +1.3 53,475 + GFF6557 0.75 +0.4 53,476 - GFF6557 0.75 -0.4 53,568 + GFF6557 0.82 -0.0 53,568 + GFF6557 0.82 -0.5 53,568 + GFF6557 0.82 -0.8 53,568 + GFF6557 0.82 +0.6 53,569 - GFF6557 0.82 +0.0 53,757 + -0.0 53,758 - -0.9 53,859 + +1.0 53,860 - +0.5 53,860 - +0.9 53,863 + +0.4 53,863 + -0.6 53,904 - +0.3 53,950 + -1.9 53,950 + -1.8 53,950 + -0.2 53,951 - -1.5 53,951 - -1.4 53,951 - +0.7 53,951 - +0.6 54,061 + -0.8 54,061 + +0.3 54,061 + -0.0 54,061 + -0.1 54,062 - -0.3 54,062 - -1.4 54,139 + GFF6558 0.14 -0.2 54,139 + GFF6558 0.14 -0.1 54,139 + GFF6558 0.14 +0.4 54,139 + GFF6558 0.14 -2.0 54,139 + GFF6558 0.14 +0.2 54,139 + GFF6558 0.14 +1.2 54,139 + GFF6558 0.14 -1.2 54,140 - GFF6558 0.14 -0.0 54,140 - GFF6558 0.14 -1.9 54,140 - GFF6558 0.14 -1.3 54,140 - GFF6558 0.14 +0.9 54,145 + GFF6558 0.14 +1.6 54,145 + GFF6558 0.14 +0.0 54,230 + GFF6558 0.19 -0.0 54,230 + GFF6558 0.19 -0.2 54,258 + GFF6558 0.20 +1.0 54,301 + GFF6558 0.23 -0.0 54,302 - GFF6558 0.23 +0.1 54,526 + GFF6558 0.35 -1.0 54,526 + GFF6558 0.35 +0.2 54,526 + GFF6558 0.35 +1.0 54,526 + GFF6558 0.35 -0.4 54,527 - GFF6558 0.35 -0.8 54,592 + GFF6558 0.39 -1.0 54,592 + GFF6558 0.39 +2.2 54,592 + GFF6558 0.39 -1.2 54,592 + GFF6558 0.39 -0.1 54,592 + GFF6558 0.39 +1.2 54,592 + GFF6558 0.39 +0.5 54,593 - GFF6558 0.39 -0.1 54,593 - GFF6558 0.39 -1.0 54,593 - GFF6558 0.39 -0.3 54,613 + GFF6558 0.40 +0.6 54,613 + GFF6558 0.40 +0.4 54,613 + GFF6558 0.40 +0.5 54,614 - GFF6558 0.40 -0.1 54,614 - GFF6558 0.40 -0.4 54,614 - GFF6558 0.40 -1.4 54,712 + GFF6558 0.45 -0.8 54,764 - GFF6558 0.48 -1.4 54,775 + GFF6558 0.49 +0.3 54,775 + GFF6558 0.49 -0.0 54,775 + GFF6558 0.49 -0.9 54,775 + GFF6558 0.49 -1.2 54,775 + GFF6558 0.49 -0.6 54,776 - GFF6558 0.49 -0.8 54,776 - GFF6558 0.49 -0.6 54,776 - GFF6558 0.49 -0.8 54,776 - GFF6558 0.49 +0.5
Or see this region's nucleotide sequence