Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6501 and GFF6502 are separated by 32 nucleotides
GFF6501 - Uncharacterized MFS-type transporter, at 77,710 to 79,119
GFF6501
GFF6502 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 79,152 to 80,150
GFF6502
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 78.272 kb on + strand, within GFF6501 at 78.272 kb on + strand, within GFF6501 at 78.272 kb on + strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.317 kb on + strand, within GFF6501 at 78.318 kb on - strand, within GFF6501 at 78.318 kb on - strand, within GFF6501 at 78.497 kb on + strand, within GFF6501 at 78.497 kb on + strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.915 kb on - strand, within GFF6501 at 78.926 kb on + strand, within GFF6501 at 78.927 kb on - strand, within GFF6501 at 79.013 kb on + strand at 79.013 kb on + strand at 79.013 kb on + strand at 79.029 kb on - strand at 79.029 kb on - strand at 79.029 kb on - strand at 79.201 kb on + strand at 79.201 kb on + strand at 79.202 kb on - strand at 79.202 kb on - strand at 79.202 kb on - strand at 79.210 kb on + strand at 79.210 kb on + strand at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.268 kb on - strand, within GFF6502 at 79.268 kb on - strand, within GFF6502 at 79.271 kb on + strand, within GFF6502 at 79.271 kb on + strand, within GFF6502 at 79.272 kb on - strand, within GFF6502 at 79.272 kb on - strand, within GFF6502 at 79.280 kb on - strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.379 kb on - strand, within GFF6502 at 79.379 kb on - strand, within GFF6502 at 79.665 kb on - strand, within GFF6502 at 79.768 kb on + strand, within GFF6502 at 79.768 kb on + strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 80.032 kb on + strand, within GFF6502 at 80.033 kb on - strand, within GFF6502 at 80.073 kb on - strand at 80.073 kb on - strand at 80.089 kb on - strand at 80.089 kb on - strand at 80.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 78,272 + GFF6501 0.40 -0.2 78,272 + GFF6501 0.40 +0.9 78,272 + GFF6501 0.40 -0.0 78,273 - GFF6501 0.40 -0.1 78,273 - GFF6501 0.40 -0.6 78,273 - GFF6501 0.40 +0.3 78,273 - GFF6501 0.40 +0.2 78,273 - GFF6501 0.40 -0.4 78,273 - GFF6501 0.40 -0.8 78,273 - GFF6501 0.40 +0.5 78,273 - GFF6501 0.40 -0.1 78,317 + GFF6501 0.43 -1.9 78,318 - GFF6501 0.43 -0.9 78,318 - GFF6501 0.43 +0.4 78,497 + GFF6501 0.56 -0.1 78,497 + GFF6501 0.56 -0.8 78,498 - GFF6501 0.56 +0.5 78,498 - GFF6501 0.56 +0.8 78,498 - GFF6501 0.56 +0.3 78,498 - GFF6501 0.56 -0.5 78,575 + GFF6501 0.61 +0.9 78,575 + GFF6501 0.61 +1.0 78,575 + GFF6501 0.61 +0.5 78,575 + GFF6501 0.61 -0.5 78,575 + GFF6501 0.61 -0.4 78,575 + GFF6501 0.61 -1.5 78,575 + GFF6501 0.61 +0.1 78,575 + GFF6501 0.61 -0.8 78,575 + GFF6501 0.61 +1.2 78,575 + GFF6501 0.61 -0.0 78,575 + GFF6501 0.61 +0.3 78,575 + GFF6501 0.61 +1.8 78,575 + GFF6501 0.61 -0.5 78,576 - GFF6501 0.61 -0.7 78,576 - GFF6501 0.61 -0.4 78,576 - GFF6501 0.61 -0.8 78,576 - GFF6501 0.61 -0.0 78,576 - GFF6501 0.61 -1.6 78,576 - GFF6501 0.61 -0.5 78,576 - GFF6501 0.61 -0.6 78,785 + GFF6501 0.76 -1.0 78,785 + GFF6501 0.76 -2.4 78,785 + GFF6501 0.76 +0.8 78,786 - GFF6501 0.76 -0.6 78,786 - GFF6501 0.76 -0.2 78,786 - GFF6501 0.76 +0.2 78,786 - GFF6501 0.76 -1.3 78,786 - GFF6501 0.76 +0.7 78,915 - GFF6501 0.85 +0.4 78,926 + GFF6501 0.86 -0.3 78,927 - GFF6501 0.86 -0.2 79,013 + -0.6 79,013 + -0.2 79,013 + -1.0 79,029 - -0.8 79,029 - -0.3 79,029 - +0.1 79,201 + -0.5 79,201 + +0.7 79,202 - -0.8 79,202 - -0.3 79,202 - -0.5 79,210 + +0.5 79,210 + -0.0 79,267 + GFF6502 0.12 -0.9 79,267 + GFF6502 0.12 -0.5 79,267 + GFF6502 0.12 +0.5 79,267 + GFF6502 0.12 -0.9 79,268 - GFF6502 0.12 -0.3 79,268 - GFF6502 0.12 -0.8 79,271 + GFF6502 0.12 +0.2 79,271 + GFF6502 0.12 +0.8 79,272 - GFF6502 0.12 -0.8 79,272 - GFF6502 0.12 -0.2 79,280 - GFF6502 0.13 +1.0 79,378 + GFF6502 0.23 +0.7 79,378 + GFF6502 0.23 +0.2 79,378 + GFF6502 0.23 +1.1 79,378 + GFF6502 0.23 +0.1 79,379 - GFF6502 0.23 -3.0 79,379 - GFF6502 0.23 -2.8 79,665 - GFF6502 0.51 -1.0 79,768 + GFF6502 0.62 -0.3 79,768 + GFF6502 0.62 +1.3 79,769 - GFF6502 0.62 +0.9 79,769 - GFF6502 0.62 +0.5 79,769 - GFF6502 0.62 -0.7 79,769 - GFF6502 0.62 +0.5 80,032 + GFF6502 0.88 -0.5 80,033 - GFF6502 0.88 -1.3 80,073 - -0.8 80,073 - -0.2 80,089 - -0.0 80,089 - +0.9 80,089 - -0.8
Or see this region's nucleotide sequence