Strain Fitness in Variovorax sp. SCN45 around GFF6445

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
20,582	+		GFF6444	0.61	+0.5
20,582	+		GFF6444	0.61	+0.1
20,583	-		GFF6444	0.61	+0.4
20,815	-		GFF6444	0.74	-0.3
20,846	+		GFF6444	0.75	-0.1
20,846	+		GFF6444	0.75	+0.3
20,994	-		GFF6444	0.83	-0.3
21,344	-				-0.2
21,357	+				-0.8
21,357	+				+0.2
22,748	+		GFF6446	0.42	-0.6
22,748	+		GFF6446	0.42	-0.5
22,748	+		GFF6446	0.42	-0.8
22,749	-		GFF6446	0.42	+1.2
22,749	-		GFF6446	0.42	-0.3
22,749	-		GFF6446	0.42	-2.2
22,749	-		GFF6446	0.42	+0.7
22,841	+		GFF6446	0.51	-0.0
22,841	+		GFF6446	0.51	+0.1
22,841	+		GFF6446	0.51	+0.8
22,841	+		GFF6446	0.51	-0.4
22,841	+		GFF6446	0.51	-1.0
22,842	-		GFF6446	0.51	-0.4
22,943	+		GFF6446	0.60	-1.3
22,944	-		GFF6446	0.60	-0.5
23,036	+		GFF6446	0.69	-0.0
23,036	+		GFF6446	0.69	-0.3
23,037	-		GFF6446	0.69	-0.5
23,273	+				-0.4
23,273	+				+0.1
23,274	-				-1.0
23,274	-				+0.5
23,274	-				+0.5
23,288	+				-0.9