Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6402 and GFF6403 are separated by 27 nucleotides GFF6403 and GFF6404 are separated by 148 nucleotides GFF6404 and GFF6405 are separated by 7 nucleotides GFF6405 and GFF6406 are separated by 49 nucleotides
GFF6402 - Imidazolonepropionase (EC 3.5.2.7), at 76,733 to 77,959
GFF6402
GFF6403 - Stresses-induced protein Ves (HutD), at 77,987 to 78,559
GFF6403
GFF6404 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 78,708 to 79,376
GFF6404
GFF6405 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 79,384 to 80,136
GFF6405
GFF6406 - Transcriptional regulator, IclR family, at 80,186 to 80,992
GFF6406
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 77.736 kb on + strand, within GFF6402 at 77.737 kb on - strand, within GFF6402 at 77.737 kb on - strand, within GFF6402 at 78.299 kb on + strand, within GFF6403 at 78.299 kb on + strand, within GFF6403 at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.695 kb on - strand at 78.695 kb on - strand at 78.695 kb on - strand at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.927 kb on + strand, within GFF6404 at 79.069 kb on + strand, within GFF6404 at 79.069 kb on + strand, within GFF6404 at 79.069 kb on + strand, within GFF6404 at 79.069 kb on + strand, within GFF6404 at 79.070 kb on - strand, within GFF6404 at 79.070 kb on - strand, within GFF6404 at 79.070 kb on - strand, within GFF6404 at 79.070 kb on - strand, within GFF6404 at 79.070 kb on - strand, within GFF6404 at 79.081 kb on + strand, within GFF6404 at 79.201 kb on + strand, within GFF6404 at 79.201 kb on + strand, within GFF6404 at 79.201 kb on + strand, within GFF6404 at 79.202 kb on - strand, within GFF6404 at 79.219 kb on + strand, within GFF6404 at 79.219 kb on + strand, within GFF6404 at 79.219 kb on + strand, within GFF6404 at 79.220 kb on - strand, within GFF6404 at 79.385 kb on + strand at 79.385 kb on + strand at 79.385 kb on + strand at 79.385 kb on + strand at 79.385 kb on + strand at 79.385 kb on + strand at 79.385 kb on + strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.386 kb on - strand at 79.427 kb on + strand at 79.427 kb on + strand at 79.427 kb on + strand at 79.427 kb on + strand at 79.427 kb on + strand at 79.428 kb on - strand at 79.428 kb on - strand at 79.428 kb on - strand at 79.428 kb on - strand at 79.428 kb on - strand at 79.428 kb on - strand at 79.511 kb on + strand, within GFF6405 at 79.511 kb on + strand, within GFF6405 at 79.511 kb on + strand, within GFF6405 at 79.512 kb on - strand, within GFF6405 at 79.568 kb on + strand, within GFF6405 at 79.568 kb on + strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.569 kb on - strand, within GFF6405 at 79.574 kb on + strand, within GFF6405 at 79.574 kb on + strand, within GFF6405 at 79.575 kb on - strand, within GFF6405 at 79.575 kb on - strand, within GFF6405 at 79.575 kb on - strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.691 kb on + strand, within GFF6405 at 79.692 kb on - strand, within GFF6405 at 79.692 kb on - strand, within GFF6405 at 79.802 kb on + strand, within GFF6405 at 79.802 kb on + strand, within GFF6405 at 79.803 kb on - strand, within GFF6405 at 79.803 kb on - strand, within GFF6405 at 79.805 kb on + strand, within GFF6405 at 79.805 kb on + strand, within GFF6405 at 79.806 kb on - strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.850 kb on + strand, within GFF6405 at 79.851 kb on - strand, within GFF6405 at 79.851 kb on - strand, within GFF6405 at 80.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 77,736 + GFF6402 0.82 +0.1 77,737 - GFF6402 0.82 +0.5 77,737 - GFF6402 0.82 +1.0 78,299 + GFF6403 0.54 -0.9 78,299 + GFF6403 0.54 -0.0 78,694 + -0.7 78,694 + +0.5 78,694 + +0.6 78,694 + -0.5 78,694 + -0.3 78,694 + +0.6 78,694 + -1.2 78,695 - +0.3 78,695 - -0.1 78,695 - -0.3 78,787 + GFF6404 0.12 +0.1 78,787 + GFF6404 0.12 +1.0 78,787 + GFF6404 0.12 -0.3 78,787 + GFF6404 0.12 -1.4 78,787 + GFF6404 0.12 +0.4 78,788 - GFF6404 0.12 +0.1 78,788 - GFF6404 0.12 -0.4 78,788 - GFF6404 0.12 -1.5 78,788 - GFF6404 0.12 -0.0 78,808 + GFF6404 0.15 -0.3 78,808 + GFF6404 0.15 -0.1 78,808 + GFF6404 0.15 +1.4 78,809 - GFF6404 0.15 +0.3 78,809 - GFF6404 0.15 +2.0 78,809 - GFF6404 0.15 -0.0 78,809 - GFF6404 0.15 -0.5 78,809 - GFF6404 0.15 +0.4 78,927 + GFF6404 0.33 -0.0 79,069 + GFF6404 0.54 -1.0 79,069 + GFF6404 0.54 -0.6 79,069 + GFF6404 0.54 +0.5 79,069 + GFF6404 0.54 -0.4 79,070 - GFF6404 0.54 +0.3 79,070 - GFF6404 0.54 +0.4 79,070 - GFF6404 0.54 -0.5 79,070 - GFF6404 0.54 +1.0 79,070 - GFF6404 0.54 -0.3 79,081 + GFF6404 0.56 -0.0 79,201 + GFF6404 0.74 -1.3 79,201 + GFF6404 0.74 -0.0 79,201 + GFF6404 0.74 +0.0 79,202 - GFF6404 0.74 -1.2 79,219 + GFF6404 0.76 +0.9 79,219 + GFF6404 0.76 -0.8 79,219 + GFF6404 0.76 +0.4 79,220 - GFF6404 0.77 -0.2 79,385 + -1.3 79,385 + +0.3 79,385 + +0.7 79,385 + +0.5 79,385 + +0.9 79,385 + +0.1 79,385 + -0.1 79,386 - -2.0 79,386 - -0.0 79,386 - -0.2 79,386 - -0.3 79,386 - +0.2 79,386 - +2.4 79,386 - -0.9 79,386 - -0.7 79,386 - -1.0 79,386 - -0.0 79,386 - -0.9 79,386 - +0.7 79,427 + +0.0 79,427 + -0.6 79,427 + -0.7 79,427 + +0.3 79,427 + -0.8 79,428 - -0.2 79,428 - -1.0 79,428 - -0.6 79,428 - +1.0 79,428 - -1.5 79,428 - -0.9 79,511 + GFF6405 0.17 +0.6 79,511 + GFF6405 0.17 +0.5 79,511 + GFF6405 0.17 -0.3 79,512 - GFF6405 0.17 +0.4 79,568 + GFF6405 0.24 -0.0 79,568 + GFF6405 0.24 +1.6 79,569 - GFF6405 0.25 +0.4 79,569 - GFF6405 0.25 +0.6 79,569 - GFF6405 0.25 +0.7 79,569 - GFF6405 0.25 -0.2 79,569 - GFF6405 0.25 +0.3 79,569 - GFF6405 0.25 -0.2 79,574 + GFF6405 0.25 -0.3 79,574 + GFF6405 0.25 +1.2 79,575 - GFF6405 0.25 -0.0 79,575 - GFF6405 0.25 -0.9 79,575 - GFF6405 0.25 +0.5 79,691 + GFF6405 0.41 -0.0 79,691 + GFF6405 0.41 -0.8 79,691 + GFF6405 0.41 -1.2 79,691 + GFF6405 0.41 -0.0 79,691 + GFF6405 0.41 +0.7 79,691 + GFF6405 0.41 -1.0 79,691 + GFF6405 0.41 +0.5 79,692 - GFF6405 0.41 -0.1 79,692 - GFF6405 0.41 +0.5 79,802 + GFF6405 0.56 +0.2 79,802 + GFF6405 0.56 -0.4 79,803 - GFF6405 0.56 -0.2 79,803 - GFF6405 0.56 -0.9 79,805 + GFF6405 0.56 -0.5 79,805 + GFF6405 0.56 -0.2 79,806 - GFF6405 0.56 +0.2 79,850 + GFF6405 0.62 -1.4 79,850 + GFF6405 0.62 +0.7 79,850 + GFF6405 0.62 +0.4 79,850 + GFF6405 0.62 -1.0 79,850 + GFF6405 0.62 -0.9 79,850 + GFF6405 0.62 -1.2 79,850 + GFF6405 0.62 +0.1 79,850 + GFF6405 0.62 -0.9 79,850 + GFF6405 0.62 +1.1 79,850 + GFF6405 0.62 -0.9 79,851 - GFF6405 0.62 +0.4 79,851 - GFF6405 0.62 -0.6 80,188 - -0.1
Or see this region's nucleotide sequence