Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF636 and GFF637 are separated by 229 nucleotides GFF637 and GFF638 are separated by 7 nucleotides GFF638 and GFF639 are separated by 118 nucleotides
GFF636 - Urea ABC transporter, substrate-binding protein UrtA, at 685,285 to 686,541
GFF636
GFF637 - Two-component system sensor histidine kinase/response regulator hybrid, at 686,771 to 690,163
GFF637
GFF638 - Two-component transcriptional response regulator, LuxR family, at 690,171 to 691,043
GFF638
GFF639 - hypothetical protein, at 691,162 to 691,932
GFF639
Position (kb)
686
687
688
689
690
691 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 685.866 kb on - strand, within GFF636 at 685.869 kb on - strand, within GFF636 at 685.869 kb on - strand, within GFF636 at 685.869 kb on - strand, within GFF636 at 685.935 kb on + strand, within GFF636 at 685.936 kb on - strand, within GFF636 at 685.949 kb on + strand, within GFF636 at 685.949 kb on + strand, within GFF636 at 686.045 kb on + strand, within GFF636 at 686.079 kb on - strand, within GFF636 at 686.094 kb on - strand, within GFF636 at 686.656 kb on - strand at 686.816 kb on + strand at 686.852 kb on + strand at 686.864 kb on + strand at 687.050 kb on + strand at 687.053 kb on + strand at 687.054 kb on - strand at 687.234 kb on - strand, within GFF637 at 687.377 kb on + strand, within GFF637 at 687.377 kb on + strand, within GFF637 at 687.377 kb on + strand, within GFF637 at 687.501 kb on - strand, within GFF637 at 687.683 kb on + strand, within GFF637 at 687.684 kb on - strand, within GFF637 at 687.702 kb on - strand, within GFF637 at 687.788 kb on + strand, within GFF637 at 687.938 kb on + strand, within GFF637 at 687.939 kb on - strand, within GFF637 at 687.939 kb on - strand, within GFF637 at 688.046 kb on + strand, within GFF637 at 688.214 kb on + strand, within GFF637 at 688.217 kb on + strand, within GFF637 at 688.218 kb on - strand, within GFF637 at 688.218 kb on - strand, within GFF637 at 688.478 kb on + strand, within GFF637 at 688.479 kb on - strand, within GFF637 at 688.479 kb on - strand, within GFF637 at 688.536 kb on - strand, within GFF637 at 688.796 kb on + strand, within GFF637 at 689.084 kb on + strand, within GFF637 at 689.085 kb on - strand, within GFF637 at 689.445 kb on - strand, within GFF637 at 689.748 kb on + strand, within GFF637 at 689.748 kb on + strand, within GFF637 at 689.749 kb on - strand, within GFF637 at 689.749 kb on - strand, within GFF637 at 689.749 kb on - strand, within GFF637 at 689.749 kb on - strand, within GFF637 at 690.255 kb on + strand at 690.325 kb on + strand, within GFF638 at 690.480 kb on + strand, within GFF638 at 690.480 kb on + strand, within GFF638 at 690.480 kb on + strand, within GFF638 at 690.481 kb on - strand, within GFF638 at 691.082 kb on - strand at 691.105 kb on - strand at 691.105 kb on - strand at 691.105 kb on - strand at 691.105 kb on - strand at 691.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 685,866 - GFF636 0.46 +0.8 685,869 - GFF636 0.46 +0.1 685,869 - GFF636 0.46 -0.8 685,869 - GFF636 0.46 +1.1 685,935 + GFF636 0.52 -0.3 685,936 - GFF636 0.52 -2.7 685,949 + GFF636 0.53 -0.2 685,949 + GFF636 0.53 +0.1 686,045 + GFF636 0.60 +0.9 686,079 - GFF636 0.63 -1.2 686,094 - GFF636 0.64 +0.2 686,656 - -1.7 686,816 + +0.1 686,852 + -0.2 686,864 + -1.1 687,050 + -1.7 687,053 + -0.2 687,054 - -1.1 687,234 - GFF637 0.14 -0.3 687,377 + GFF637 0.18 +0.4 687,377 + GFF637 0.18 +0.5 687,377 + GFF637 0.18 +1.6 687,501 - GFF637 0.22 +0.1 687,683 + GFF637 0.27 +0.3 687,684 - GFF637 0.27 -0.9 687,702 - GFF637 0.27 -0.6 687,788 + GFF637 0.30 -1.3 687,938 + GFF637 0.34 +0.1 687,939 - GFF637 0.34 -0.1 687,939 - GFF637 0.34 +1.9 688,046 + GFF637 0.38 -0.1 688,214 + GFF637 0.43 -2.6 688,217 + GFF637 0.43 +0.5 688,218 - GFF637 0.43 +0.2 688,218 - GFF637 0.43 +1.6 688,478 + GFF637 0.50 -1.4 688,479 - GFF637 0.50 -1.8 688,479 - GFF637 0.50 -1.7 688,536 - GFF637 0.52 +0.2 688,796 + GFF637 0.60 +0.4 689,084 + GFF637 0.68 -1.0 689,085 - GFF637 0.68 -0.4 689,445 - GFF637 0.79 +0.1 689,748 + GFF637 0.88 -1.1 689,748 + GFF637 0.88 -0.6 689,749 - GFF637 0.88 -1.0 689,749 - GFF637 0.88 +1.7 689,749 - GFF637 0.88 +1.2 689,749 - GFF637 0.88 -0.5 690,255 + -0.3 690,325 + GFF638 0.18 +1.1 690,480 + GFF638 0.35 -0.8 690,480 + GFF638 0.35 +0.6 690,480 + GFF638 0.35 +0.2 690,481 - GFF638 0.36 +0.4 691,082 - -0.1 691,105 - +0.8 691,105 - -1.0 691,105 - +0.6 691,105 - -0.1 691,106 + -0.8
Or see this region's nucleotide sequence