Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6301 and GFF6302 are separated by 126 nucleotides GFF6302 and GFF6303 are separated by 46 nucleotides
GFF6301 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 90,255 to 91,193
GFF6301
GFF6302 - Transcriptional regulator, IclR family, at 91,320 to 92,060
GFF6302
GFF6303 - BUG/TctC family periplasmic protein, at 92,107 to 93,078
GFF6303
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 90.322 kb on + strand at 90.322 kb on + strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.466 kb on + strand, within GFF6301 at 90.466 kb on + strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.647 kb on - strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.271 kb on - strand at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.548 kb on + strand, within GFF6302 at 91.548 kb on + strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.998 kb on + strand at 91.998 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.173 kb on + strand at 92.173 kb on + strand at 92.173 kb on + strand at 92.174 kb on - strand at 92.174 kb on - strand at 92.225 kb on + strand, within GFF6303 at 92.225 kb on + strand, within GFF6303 at 92.225 kb on + strand, within GFF6303 at 92.225 kb on + strand, within GFF6303 at 92.225 kb on + strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.226 kb on - strand, within GFF6303 at 92.318 kb on + strand, within GFF6303 at 92.318 kb on + strand, within GFF6303 at 92.318 kb on + strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.319 kb on - strand, within GFF6303 at 92.349 kb on - strand, within GFF6303 at 92.528 kb on + strand, within GFF6303 at 92.529 kb on - strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.615 kb on + strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.616 kb on - strand, within GFF6303 at 92.754 kb on - strand, within GFF6303 at 92.769 kb on - strand, within GFF6303
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 90,322 + +0.9 90,322 + -1.5 90,323 - -1.0 90,323 - -1.2 90,323 - -0.0 90,466 + GFF6301 0.22 -0.8 90,466 + GFF6301 0.22 +0.1 90,467 - GFF6301 0.23 -0.2 90,467 - GFF6301 0.23 -0.8 90,467 - GFF6301 0.23 +0.2 90,646 + GFF6301 0.42 +0.3 90,646 + GFF6301 0.42 -0.3 90,646 + GFF6301 0.42 -0.8 90,646 + GFF6301 0.42 +0.5 90,646 + GFF6301 0.42 -0.7 90,646 + GFF6301 0.42 -0.2 90,646 + GFF6301 0.42 +0.4 90,647 - GFF6301 0.42 -0.6 91,084 + GFF6301 0.88 -0.2 91,084 + GFF6301 0.88 -0.6 91,084 + GFF6301 0.88 -0.3 91,085 - GFF6301 0.88 +0.1 91,085 - GFF6301 0.88 -0.3 91,085 - GFF6301 0.88 -0.3 91,271 - -0.5 91,473 + GFF6302 0.21 +0.6 91,473 + GFF6302 0.21 -1.5 91,473 + GFF6302 0.21 +0.0 91,473 + GFF6302 0.21 -0.7 91,473 + GFF6302 0.21 -1.9 91,473 + GFF6302 0.21 -0.6 91,473 + GFF6302 0.21 -0.3 91,473 + GFF6302 0.21 -0.7 91,473 + GFF6302 0.21 -0.2 91,474 - GFF6302 0.21 -0.9 91,474 - GFF6302 0.21 -0.8 91,474 - GFF6302 0.21 -1.1 91,474 - GFF6302 0.21 +0.1 91,474 - GFF6302 0.21 +0.2 91,474 - GFF6302 0.21 -0.9 91,474 - GFF6302 0.21 -0.2 91,474 - GFF6302 0.21 -1.1 91,474 - GFF6302 0.21 +0.8 91,474 - GFF6302 0.21 -0.8 91,474 - GFF6302 0.21 +1.1 91,474 - GFF6302 0.21 -0.2 91,548 + GFF6302 0.31 -0.5 91,548 + GFF6302 0.31 -0.1 91,549 - GFF6302 0.31 -0.7 91,549 - GFF6302 0.31 +0.3 91,549 - GFF6302 0.31 -0.2 91,587 + GFF6302 0.36 +0.4 91,587 + GFF6302 0.36 -0.3 91,587 + GFF6302 0.36 -0.0 91,587 + GFF6302 0.36 -1.0 91,587 + GFF6302 0.36 -0.8 91,588 - GFF6302 0.36 +1.5 91,588 - GFF6302 0.36 -0.9 91,588 - GFF6302 0.36 +0.2 91,588 - GFF6302 0.36 -0.2 91,668 + GFF6302 0.47 -0.9 91,668 + GFF6302 0.47 -0.2 91,668 + GFF6302 0.47 -0.8 91,668 + GFF6302 0.47 -0.3 91,668 + GFF6302 0.47 -0.5 91,668 + GFF6302 0.47 +0.5 91,669 - GFF6302 0.47 -2.0 91,669 - GFF6302 0.47 +1.2 91,669 - GFF6302 0.47 -1.3 91,669 - GFF6302 0.47 -1.2 91,669 - GFF6302 0.47 -0.2 91,669 - GFF6302 0.47 +0.3 91,669 - GFF6302 0.47 -0.3 91,669 - GFF6302 0.47 -0.8 91,860 + GFF6302 0.73 -0.3 91,860 + GFF6302 0.73 +0.3 91,860 + GFF6302 0.73 +1.3 91,860 + GFF6302 0.73 -0.5 91,860 + GFF6302 0.73 +0.3 91,860 + GFF6302 0.73 -0.9 91,860 + GFF6302 0.73 -2.3 91,861 - GFF6302 0.73 -0.4 91,861 - GFF6302 0.73 +0.3 91,861 - GFF6302 0.73 -1.7 91,861 - GFF6302 0.73 -0.9 91,861 - GFF6302 0.73 -1.2 91,998 + -0.6 91,998 + -1.1 92,010 + -2.5 92,010 + -0.6 92,010 + -0.6 92,010 + -0.2 92,010 + -1.0 92,010 + +0.6 92,010 + +0.8 92,010 + -0.9 92,010 + -0.0 92,011 - +1.4 92,011 - -0.0 92,011 - +0.0 92,011 - -0.4 92,011 - -0.0 92,011 - -0.2 92,173 + -0.4 92,173 + +0.1 92,173 + -2.8 92,174 - +0.4 92,174 - +0.6 92,225 + GFF6303 0.12 -0.4 92,225 + GFF6303 0.12 -0.1 92,225 + GFF6303 0.12 +0.7 92,225 + GFF6303 0.12 +0.6 92,225 + GFF6303 0.12 -0.6 92,226 - GFF6303 0.12 -0.2 92,226 - GFF6303 0.12 -0.7 92,226 - GFF6303 0.12 -1.8 92,226 - GFF6303 0.12 -0.3 92,226 - GFF6303 0.12 +0.5 92,226 - GFF6303 0.12 -0.1 92,226 - GFF6303 0.12 -0.9 92,318 + GFF6303 0.22 +1.1 92,318 + GFF6303 0.22 -0.1 92,318 + GFF6303 0.22 +0.5 92,319 - GFF6303 0.22 -0.8 92,319 - GFF6303 0.22 -0.8 92,319 - GFF6303 0.22 +0.1 92,319 - GFF6303 0.22 -0.8 92,319 - GFF6303 0.22 +0.3 92,319 - GFF6303 0.22 -0.7 92,319 - GFF6303 0.22 +0.9 92,349 - GFF6303 0.25 +0.1 92,528 + GFF6303 0.43 -1.1 92,529 - GFF6303 0.43 -0.9 92,615 + GFF6303 0.52 -0.6 92,615 + GFF6303 0.52 -0.2 92,615 + GFF6303 0.52 -0.4 92,615 + GFF6303 0.52 -0.4 92,615 + GFF6303 0.52 +0.8 92,615 + GFF6303 0.52 +0.9 92,615 + GFF6303 0.52 -0.3 92,615 + GFF6303 0.52 +0.9 92,615 + GFF6303 0.52 -0.5 92,615 + GFF6303 0.52 -0.5 92,615 + GFF6303 0.52 -1.6 92,615 + GFF6303 0.52 -0.5 92,615 + GFF6303 0.52 -0.7 92,616 - GFF6303 0.52 -0.3 92,616 - GFF6303 0.52 +1.1 92,616 - GFF6303 0.52 -0.1 92,616 - GFF6303 0.52 +0.4 92,616 - GFF6303 0.52 +0.2 92,616 - GFF6303 0.52 +0.9 92,616 - GFF6303 0.52 -0.0 92,616 - GFF6303 0.52 -0.4 92,616 - GFF6303 0.52 -0.9 92,754 - GFF6303 0.67 -0.9 92,769 - GFF6303 0.68 -0.7
Or see this region's nucleotide sequence