Strain Fitness in Variovorax sp. SCN45 around GFF6258

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6256 and GFF6257 overlap by 4 nucleotidesGFF6257 and GFF6258 overlap by 4 nucleotidesGFF6258 and GFF6259 overlap by 4 nucleotides GFF6256 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (EC 2.7.8.37), at 49,985 to 50,698 GFF6256 GFF6257 - Phosphonates utilization ATP-binding protein PhnK, at 50,695 to 51,483 GFF6257 GFF6258 - Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase (EC 4.7.1.1), at 51,480 to 52,352 GFF6258 GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446 GFF6259 Position (kb) 51 52 53Strain fitness (log2 ratio) -2 -1 0 1 2 3at 50.514 kb on + strand, within GFF6256at 50.515 kb on - strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.625 kb on - strand, within GFF6256at 50.732 kb on + strandat 50.732 kb on + strandat 50.732 kb on + strandat 50.733 kb on - strandat 51.426 kb on - strandat 51.697 kb on + strand, within GFF6258at 51.698 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.842 kb on - strand, within GFF6258at 51.842 kb on - strand, within GFF6258at 51.880 kb on + strand, within GFF6258at 51.986 kb on - strand, within GFF6258at 52.322 kb on - strandat 52.322 kb on - strandat 52.333 kb on + strandat 52.333 kb on + strandat 52.333 kb on + strandat 52.334 kb on - strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.603 kb on - strand, within GFF6259at 52.794 kb on + strand, within GFF6259at 52.795 kb on - strand, within GFF6259at 52.932 kb on - strand, within GFF6259at 52.932 kb on - strand, within GFF6259at 53.076 kb on + strand, within GFF6259at 53.077 kb on - strand, within GFF6259at 53.077 kb on - strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.083 kb on - strand, within GFF6259at 53.083 kb on - strand, within GFF6259at 53.206 kb on - strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.284 kb on - strand, within GFF6259

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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50,514 + GFF6256 0.74 -0.7
50,515 - GFF6256 0.74 +0.3
50,523 + GFF6256 0.75 +0.3
50,523 + GFF6256 0.75 -1.0
50,523 + GFF6256 0.75 -0.6
50,625 - GFF6256 0.90 -0.4
50,732 + +0.5
50,732 + +1.7
50,732 + -0.0
50,733 - -0.5
51,426 - -0.8
51,697 + GFF6258 0.25 +0.5
51,698 - GFF6258 0.25 -0.1
51,713 - GFF6258 0.27 +1.7
51,713 - GFF6258 0.27 +0.3
51,713 - GFF6258 0.27 -1.5
51,713 - GFF6258 0.27 -1.0
51,841 + GFF6258 0.41 +0.4
51,841 + GFF6258 0.41 -0.6
51,841 + GFF6258 0.41 -0.5
51,841 + GFF6258 0.41 -0.7
51,841 + GFF6258 0.41 +0.3
51,841 + GFF6258 0.41 +0.1
51,842 - GFF6258 0.41 +0.4
51,842 - GFF6258 0.41 -1.4
51,880 + GFF6258 0.46 +0.6
51,986 - GFF6258 0.58 +3.7
52,322 - -0.7
52,322 - +0.3
52,333 + -0.1
52,333 + -1.5
52,333 + +0.7
52,334 - -0.9
52,419 + +1.1
52,419 + -0.1
52,419 + +1.0
52,419 + -0.2
52,419 + +0.9
52,419 + -0.2
52,419 + +0.0
52,419 + +0.1
52,603 - GFF6259 0.23 -1.1
52,794 + GFF6259 0.41 -0.6
52,795 - GFF6259 0.41 +0.1
52,932 - GFF6259 0.53 +1.1
52,932 - GFF6259 0.53 -0.6
53,076 + GFF6259 0.66 -0.7
53,077 - GFF6259 0.66 +0.2
53,077 - GFF6259 0.66 -0.4
53,082 + GFF6259 0.67 -0.1
53,082 + GFF6259 0.67 +0.5
53,082 + GFF6259 0.67 +0.9
53,083 - GFF6259 0.67 -0.5
53,083 - GFF6259 0.67 +0.8
53,206 - GFF6259 0.78 -0.8
53,283 + GFF6259 0.85 +0.2
53,283 + GFF6259 0.85 -0.1
53,283 + GFF6259 0.85 -2.5
53,283 + GFF6259 0.85 +0.2
53,284 - GFF6259 0.85 +1.1

Or see this region's nucleotide sequence