Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6146 and GFF6147 are separated by 23 nucleotides GFF6147 and GFF6148 are separated by 6 nucleotides GFF6148 and GFF6149 are separated by 38 nucleotides
GFF6146 - Phosphonoacetate hydrolase (EC 3.11.1.2), at 50,261 to 51,487
GFF6146
GFF6147 - Phosphonoacetaldehyde dehydrogenase (NAD+), at 51,511 to 52,959
GFF6147
GFF6148 - hypothetical protein, at 52,966 to 53,532
GFF6148
GFF6149 - FAD-dependent oxidoreductase, at 53,571 to 54,959
GFF6149
Position (kb) 51
52
53 Strain fitness (log2 ratio) -2
-1
0
1 at 50.519 kb on + strand, within GFF6146 at 50.519 kb on + strand, within GFF6146 at 50.571 kb on - strand, within GFF6146 at 50.777 kb on + strand, within GFF6146 at 50.777 kb on + strand, within GFF6146 at 50.853 kb on - strand, within GFF6146 at 50.853 kb on - strand, within GFF6146 at 51.035 kb on + strand, within GFF6146 at 51.035 kb on + strand, within GFF6146 at 51.035 kb on + strand, within GFF6146 at 51.063 kb on - strand, within GFF6146 at 51.149 kb on + strand, within GFF6146 at 51.150 kb on - strand, within GFF6146 at 51.150 kb on - strand, within GFF6146 at 51.663 kb on - strand, within GFF6147 at 51.721 kb on + strand, within GFF6147 at 52.108 kb on + strand, within GFF6147 at 52.108 kb on + strand, within GFF6147 at 52.109 kb on - strand, within GFF6147 at 52.607 kb on - strand, within GFF6147 at 52.607 kb on - strand, within GFF6147 at 52.761 kb on + strand, within GFF6147 at 53.023 kb on + strand, within GFF6148 at 53.476 kb on + strand at 53.476 kb on + strand at 53.476 kb on + strand at 53.477 kb on - strand at 53.477 kb on - strand at 53.480 kb on - strand at 53.492 kb on + strand at 53.695 kb on + strand at 53.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 50,519 + GFF6146 0.21 -0.1 50,519 + GFF6146 0.21 -0.0 50,571 - GFF6146 0.25 +0.3 50,777 + GFF6146 0.42 -0.4 50,777 + GFF6146 0.42 +1.6 50,853 - GFF6146 0.48 -2.4 50,853 - GFF6146 0.48 +0.4 51,035 + GFF6146 0.63 -1.0 51,035 + GFF6146 0.63 +1.1 51,035 + GFF6146 0.63 +0.2 51,063 - GFF6146 0.65 -0.4 51,149 + GFF6146 0.72 +0.8 51,150 - GFF6146 0.72 +0.8 51,150 - GFF6146 0.72 -0.6 51,663 - GFF6147 0.10 +0.3 51,721 + GFF6147 0.14 -0.1 52,108 + GFF6147 0.41 +0.3 52,108 + GFF6147 0.41 -0.1 52,109 - GFF6147 0.41 +0.6 52,607 - GFF6147 0.76 +0.3 52,607 - GFF6147 0.76 -0.1 52,761 + GFF6147 0.86 -0.7 53,023 + GFF6148 0.10 -0.1 53,476 + -0.9 53,476 + -1.1 53,476 + +1.6 53,477 - +0.9 53,477 - +0.1 53,480 - +0.9 53,492 + -1.5 53,695 + +1.0 53,695 + +0.1
Or see this region's nucleotide sequence