Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6009 and GFF6010 are separated by 10 nucleotides GFF6010 and GFF6011 are separated by 6 nucleotides GFF6011 and GFF6012 are separated by 111 nucleotides
GFF6009 - Acyl-CoA dehydrogenase STM0857, at 68,617 to 69,780
GFF6009
GFF6010 - MmgE/PrpD family protein, at 69,791 to 71,152
GFF6010
GFF6011 - putative decarboxylase, at 71,159 to 71,866
GFF6011
GFF6012 - Tricarboxylate transport protein TctC, at 71,978 to 72,982
GFF6012
Position (kb)
69
70
71
72 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.938 kb on + strand, within GFF6009 at 69.055 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.416 kb on - strand, within GFF6009 at 69.524 kb on - strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.587 kb on - strand, within GFF6009 at 69.701 kb on - strand at 69.701 kb on - strand at 69.739 kb on + strand at 69.739 kb on + strand at 69.743 kb on + strand at 69.744 kb on - strand at 69.999 kb on + strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.242 kb on - strand, within GFF6010 at 70.242 kb on - strand, within GFF6010 at 70.598 kb on + strand, within GFF6010 at 70.805 kb on + strand, within GFF6010 at 70.805 kb on + strand, within GFF6010 at 70.914 kb on - strand, within GFF6010 at 71.057 kb on + strand at 71.057 kb on + strand at 71.058 kb on - strand at 71.122 kb on + strand at 71.463 kb on + strand, within GFF6011 at 71.497 kb on - strand, within GFF6011 at 71.522 kb on + strand, within GFF6011 at 71.523 kb on - strand, within GFF6011 at 71.731 kb on - strand, within GFF6011 at 71.873 kb on - strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.999 kb on - strand at 72.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 68,887 + GFF6009 0.23 -0.5 68,887 + GFF6009 0.23 -0.2 68,887 + GFF6009 0.23 -2.1 68,887 + GFF6009 0.23 -0.5 68,888 - GFF6009 0.23 +0.3 68,888 - GFF6009 0.23 -1.0 68,888 - GFF6009 0.23 -0.5 68,938 + GFF6009 0.28 -0.3 69,055 + GFF6009 0.38 +0.1 69,325 + GFF6009 0.61 -0.7 69,325 + GFF6009 0.61 +1.2 69,325 + GFF6009 0.61 -0.1 69,326 - GFF6009 0.61 +0.0 69,326 - GFF6009 0.61 +0.1 69,416 - GFF6009 0.69 -1.3 69,524 - GFF6009 0.78 -0.2 69,586 + GFF6009 0.83 +0.5 69,586 + GFF6009 0.83 -0.3 69,587 - GFF6009 0.83 -0.3 69,701 - +0.9 69,701 - -0.4 69,739 + +0.5 69,739 + +1.1 69,743 + -0.5 69,744 - -1.5 69,999 + GFF6010 0.15 -0.3 70,000 - GFF6010 0.15 -1.5 70,000 - GFF6010 0.15 -0.3 70,000 - GFF6010 0.15 +0.5 70,242 - GFF6010 0.33 +0.1 70,242 - GFF6010 0.33 -1.1 70,598 + GFF6010 0.59 -0.5 70,805 + GFF6010 0.74 -0.0 70,805 + GFF6010 0.74 +0.2 70,914 - GFF6010 0.82 -0.9 71,057 + -0.6 71,057 + -0.2 71,058 - -0.1 71,122 + +1.3 71,463 + GFF6011 0.43 +0.3 71,497 - GFF6011 0.48 -0.5 71,522 + GFF6011 0.51 -0.3 71,523 - GFF6011 0.51 -1.5 71,731 - GFF6011 0.81 +0.3 71,873 - -0.4 71,998 + +0.5 71,998 + -0.2 71,998 + +1.0 71,999 - +1.6 72,075 + -0.4
Or see this region's nucleotide sequence