Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5938 and GFF5939 are separated by 8 nucleotides GFF5939 and GFF5940 overlap by 8 nucleotides GFF5940 and GFF5941 are separated by 28 nucleotides GFF5941 and GFF5942 are separated by 49 nucleotides
GFF5938 - T6SS component TssF (ImpG/VasA), at 158,220 to 160,112
GFF5938
GFF5939 - T6SS lysozyme-like component TssE, at 160,121 to 160,636
GFF5939
GFF5940 - T6SS associated component TagJ (ImpE), at 160,629 to 161,453
GFF5940
GFF5941 - hypothetical protein, at 161,482 to 162,039
GFF5941
GFF5942 - hypothetical protein, at 162,089 to 164,233
GFF5942
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.662 kb on - strand, within GFF5938 at 159.662 kb on - strand, within GFF5938 at 159.671 kb on - strand, within GFF5938 at 160.122 kb on + strand at 160.122 kb on + strand at 160.122 kb on + strand at 160.122 kb on + strand at 160.123 kb on - strand at 160.123 kb on - strand at 160.175 kb on + strand, within GFF5939 at 160.399 kb on + strand, within GFF5939 at 160.410 kb on + strand, within GFF5939 at 160.957 kb on + strand, within GFF5940 at 160.958 kb on - strand, within GFF5940 at 160.960 kb on + strand, within GFF5940 at 160.960 kb on + strand, within GFF5940 at 160.960 kb on + strand, within GFF5940 at 160.961 kb on - strand, within GFF5940 at 161.239 kb on + strand, within GFF5940 at 161.240 kb on - strand, within GFF5940 at 161.240 kb on - strand, within GFF5940 at 161.567 kb on + strand, within GFF5941 at 161.988 kb on - strand at 162.255 kb on + strand at 162.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 159,662 - GFF5938 0.76 -0.1 159,662 - GFF5938 0.76 +0.4 159,671 - GFF5938 0.77 -1.6 160,122 + -0.6 160,122 + -1.0 160,122 + +0.3 160,122 + +1.1 160,123 - +2.4 160,123 - -0.1 160,175 + GFF5939 0.10 +0.5 160,399 + GFF5939 0.54 -0.2 160,410 + GFF5939 0.56 -1.2 160,957 + GFF5940 0.40 -0.1 160,958 - GFF5940 0.40 +0.2 160,960 + GFF5940 0.40 -1.6 160,960 + GFF5940 0.40 +0.6 160,960 + GFF5940 0.40 -2.2 160,961 - GFF5940 0.40 -1.1 161,239 + GFF5940 0.74 -0.5 161,240 - GFF5940 0.74 +0.0 161,240 - GFF5940 0.74 -0.4 161,567 + GFF5941 0.15 -0.1 161,988 - -0.1 162,255 + +0.6 162,255 + -0.1
Or see this region's nucleotide sequence