Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5913 and GFF5914 overlap by 4 nucleotides GFF5914 and GFF5915 are separated by 18 nucleotides GFF5915 and GFF5916 are separated by 71 nucleotides
GFF5913 - T6SS outer membrane component TssL (ImpK/VasF) / unknown domain, at 128,254 to 129,597
GFF5913
GFF5914 - hypothetical protein, at 129,594 to 130,706
GFF5914
GFF5915 - T6SS secretion lipoprotein TssJ (VasD), at 130,725 to 131,237
GFF5915
GFF5916 - T6SS component TssA (ImpA), at 131,309 to 132,349
GFF5916
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1
2 at 128.626 kb on + strand, within GFF5913 at 128.663 kb on - strand, within GFF5913 at 128.663 kb on - strand, within GFF5913 at 128.785 kb on + strand, within GFF5913 at 128.786 kb on - strand, within GFF5913 at 128.884 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.281 kb on - strand, within GFF5913 at 129.295 kb on + strand, within GFF5913 at 129.506 kb on - strand at 130.230 kb on + strand, within GFF5914 at 130.230 kb on + strand, within GFF5914 at 130.230 kb on + strand, within GFF5914 at 130.231 kb on - strand, within GFF5914 at 130.726 kb on + strand at 131.020 kb on + strand, within GFF5915 at 131.021 kb on - strand, within GFF5915 at 131.206 kb on + strand at 131.223 kb on - strand at 131.282 kb on + strand at 131.487 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.641 kb on - strand, within GFF5916
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 128,626 + GFF5913 0.28 +1.1 128,663 - GFF5913 0.30 -0.0 128,663 - GFF5913 0.30 -0.5 128,785 + GFF5913 0.40 -0.2 128,786 - GFF5913 0.40 -0.2 128,884 + GFF5913 0.47 +0.8 129,280 + GFF5913 0.76 +0.0 129,280 + GFF5913 0.76 +0.2 129,280 + GFF5913 0.76 +0.2 129,281 - GFF5913 0.76 +2.2 129,295 + GFF5913 0.77 +0.2 129,506 - +0.1 130,230 + GFF5914 0.57 +1.4 130,230 + GFF5914 0.57 +2.6 130,230 + GFF5914 0.57 +0.0 130,231 - GFF5914 0.57 +0.2 130,726 + -2.0 131,020 + GFF5915 0.58 +0.4 131,021 - GFF5915 0.58 -0.5 131,206 + -0.2 131,223 - +1.0 131,282 + -1.0 131,487 + GFF5916 0.17 -0.3 131,640 + GFF5916 0.32 -0.7 131,640 + GFF5916 0.32 -0.2 131,640 + GFF5916 0.32 +0.6 131,641 - GFF5916 0.32 +1.7
Or see this region's nucleotide sequence