Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5870 and GFF5871 are separated by 10 nucleotides GFF5871 and GFF5872 are separated by 449 nucleotides
GFF5870 - hypothetical protein, at 82,880 to 83,734
GFF5870
GFF5871 - hypothetical protein, at 83,745 to 86,036
GFF5871
GFF5872 - Inner membrane protein YbhQ, at 86,486 to 87,520
GFF5872
Position (kb) 83
84
85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 82.827 kb on - strand at 82.837 kb on + strand at 82.837 kb on + strand at 82.837 kb on + strand at 82.837 kb on + strand at 82.837 kb on + strand at 82.838 kb on - strand at 82.838 kb on - strand at 82.838 kb on - strand at 82.838 kb on - strand at 82.838 kb on - strand at 82.867 kb on + strand at 82.867 kb on + strand at 82.867 kb on + strand at 82.868 kb on - strand at 82.868 kb on - strand at 82.868 kb on - strand at 82.868 kb on - strand at 82.886 kb on + strand at 82.886 kb on + strand at 82.886 kb on + strand at 82.887 kb on - strand at 82.887 kb on - strand at 82.887 kb on - strand at 83.210 kb on + strand, within GFF5870 at 83.210 kb on + strand, within GFF5870 at 83.210 kb on + strand, within GFF5870 at 83.210 kb on + strand, within GFF5870 at 83.210 kb on + strand, within GFF5870 at 83.210 kb on + strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.211 kb on - strand, within GFF5870 at 83.495 kb on + strand, within GFF5870 at 83.496 kb on - strand, within GFF5870 at 83.496 kb on - strand, within GFF5870 at 83.496 kb on - strand, within GFF5870 at 83.651 kb on + strand at 83.652 kb on - strand at 83.652 kb on - strand at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.056 kb on + strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.057 kb on - strand, within GFF5871 at 84.360 kb on + strand, within GFF5871 at 84.360 kb on + strand, within GFF5871 at 84.361 kb on - strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.438 kb on + strand, within GFF5871 at 84.439 kb on - strand, within GFF5871 at 84.439 kb on - strand, within GFF5871 at 84.439 kb on - strand, within GFF5871 at 84.439 kb on - strand, within GFF5871 at 84.439 kb on - strand, within GFF5871 at 84.450 kb on + strand, within GFF5871 at 84.450 kb on + strand, within GFF5871 at 84.450 kb on + strand, within GFF5871 at 84.451 kb on - strand, within GFF5871 at 84.451 kb on - strand, within GFF5871 at 84.451 kb on - strand, within GFF5871 at 84.451 kb on - strand, within GFF5871 at 84.451 kb on - strand, within GFF5871 at 84.502 kb on - strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.552 kb on + strand, within GFF5871 at 84.553 kb on - strand, within GFF5871 at 84.553 kb on - strand, within GFF5871 at 84.662 kb on + strand, within GFF5871 at 84.664 kb on - strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.738 kb on + strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.739 kb on - strand, within GFF5871 at 84.864 kb on + strand, within GFF5871 at 84.864 kb on + strand, within GFF5871 at 84.864 kb on + strand, within GFF5871 at 84.865 kb on - strand, within GFF5871 at 85.022 kb on + strand, within GFF5871 at 85.022 kb on + strand, within GFF5871 at 85.023 kb on - strand, within GFF5871 at 85.023 kb on - strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.086 kb on + strand, within GFF5871 at 85.087 kb on - strand, within GFF5871 at 85.087 kb on - strand, within GFF5871 at 85.087 kb on - strand, within GFF5871 at 85.087 kb on - strand, within GFF5871 at 85.203 kb on + strand, within GFF5871 at 85.203 kb on + strand, within GFF5871 at 85.203 kb on + strand, within GFF5871 at 85.236 kb on + strand, within GFF5871 at 85.237 kb on - strand, within GFF5871 at 85.237 kb on - strand, within GFF5871 at 85.317 kb on + strand, within GFF5871 at 86.080 kb on + strand at 86.080 kb on + strand at 86.080 kb on + strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.081 kb on - strand at 86.083 kb on + strand at 86.083 kb on + strand at 86.083 kb on + strand at 86.084 kb on - strand at 86.269 kb on + strand at 86.269 kb on + strand at 86.269 kb on + strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.270 kb on - strand at 86.562 kb on + strand at 86.562 kb on + strand at 86.562 kb on + strand at 86.562 kb on + strand at 86.563 kb on - strand at 86.563 kb on - strand at 86.563 kb on - strand at 86.563 kb on - strand at 86.580 kb on + strand at 86.580 kb on + strand at 86.581 kb on - strand at 86.581 kb on - strand at 86.581 kb on - strand at 86.581 kb on - strand at 86.581 kb on - strand at 86.581 kb on - strand at 86.613 kb on + strand, within GFF5872 at 86.613 kb on + strand, within GFF5872 at 86.613 kb on + strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.614 kb on - strand, within GFF5872 at 86.616 kb on + strand, within GFF5872 at 86.616 kb on + strand, within GFF5872 at 86.616 kb on + strand, within GFF5872 at 86.616 kb on + strand, within GFF5872 at 86.616 kb on + strand, within GFF5872 at 86.617 kb on - strand, within GFF5872 at 86.617 kb on - strand, within GFF5872 at 86.617 kb on - strand, within GFF5872 at 86.628 kb on + strand, within GFF5872 at 86.628 kb on + strand, within GFF5872 at 86.688 kb on + strand, within GFF5872 at 86.689 kb on - strand, within GFF5872 at 86.689 kb on - strand, within GFF5872 at 86.689 kb on - strand, within GFF5872 at 86.689 kb on - strand, within GFF5872 at 86.689 kb on - strand, within GFF5872 at 86.778 kb on + strand, within GFF5872 at 86.779 kb on - strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.805 kb on + strand, within GFF5872 at 86.806 kb on - strand, within GFF5872 at 86.806 kb on - strand, within GFF5872 at 86.806 kb on - strand, within GFF5872 at 86.806 kb on - strand, within GFF5872 at 86.806 kb on - strand, within GFF5872 at 86.943 kb on + strand, within GFF5872 at 86.943 kb on + strand, within GFF5872 at 86.943 kb on + strand, within GFF5872 at 86.943 kb on + strand, within GFF5872 at 86.943 kb on + strand, within GFF5872 at 86.944 kb on - strand, within GFF5872 at 86.944 kb on - strand, within GFF5872 at 86.944 kb on - strand, within GFF5872 at 86.944 kb on - strand, within GFF5872 at 86.944 kb on - strand, within GFF5872 at 86.944 kb on - strand, within GFF5872
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 82,827 - -1.2 82,837 + -1.4 82,837 + -1.0 82,837 + -1.2 82,837 + -1.9 82,837 + +0.2 82,838 - -0.4 82,838 - -0.2 82,838 - -0.6 82,838 - -0.5 82,838 - -0.1 82,867 + -0.9 82,867 + -0.2 82,867 + -0.2 82,868 - +1.0 82,868 - -0.2 82,868 - +2.2 82,868 - +0.4 82,886 + -0.9 82,886 + -0.3 82,886 + -0.1 82,887 - +0.7 82,887 - +0.8 82,887 - -0.8 83,210 + GFF5870 0.39 -0.5 83,210 + GFF5870 0.39 -1.3 83,210 + GFF5870 0.39 -0.5 83,210 + GFF5870 0.39 -0.6 83,210 + GFF5870 0.39 -2.3 83,210 + GFF5870 0.39 -0.7 83,211 - GFF5870 0.39 -0.9 83,211 - GFF5870 0.39 -0.8 83,211 - GFF5870 0.39 -0.7 83,211 - GFF5870 0.39 -0.4 83,211 - GFF5870 0.39 -2.3 83,211 - GFF5870 0.39 -1.0 83,495 + GFF5870 0.72 -1.1 83,496 - GFF5870 0.72 +0.2 83,496 - GFF5870 0.72 -0.5 83,496 - GFF5870 0.72 -1.7 83,651 + +0.4 83,652 - -0.2 83,652 - -1.9 84,056 + GFF5871 0.14 -0.7 84,056 + GFF5871 0.14 -1.0 84,056 + GFF5871 0.14 -1.5 84,056 + GFF5871 0.14 +0.2 84,056 + GFF5871 0.14 -0.5 84,056 + GFF5871 0.14 -0.8 84,056 + GFF5871 0.14 -1.4 84,056 + GFF5871 0.14 +0.8 84,056 + GFF5871 0.14 -0.4 84,056 + GFF5871 0.14 -0.6 84,056 + GFF5871 0.14 -0.6 84,056 + GFF5871 0.14 +0.1 84,056 + GFF5871 0.14 -1.0 84,056 + GFF5871 0.14 -0.2 84,056 + GFF5871 0.14 -2.4 84,056 + GFF5871 0.14 -0.0 84,056 + GFF5871 0.14 -2.4 84,056 + GFF5871 0.14 -1.2 84,056 + GFF5871 0.14 -0.2 84,056 + GFF5871 0.14 -0.4 84,056 + GFF5871 0.14 -1.1 84,057 - GFF5871 0.14 -1.2 84,057 - GFF5871 0.14 -0.2 84,057 - GFF5871 0.14 -1.7 84,057 - GFF5871 0.14 +0.1 84,057 - GFF5871 0.14 -0.4 84,057 - GFF5871 0.14 -0.9 84,057 - GFF5871 0.14 -0.9 84,057 - GFF5871 0.14 -0.0 84,057 - GFF5871 0.14 -2.9 84,057 - GFF5871 0.14 +0.5 84,057 - GFF5871 0.14 -0.4 84,057 - GFF5871 0.14 -1.3 84,057 - GFF5871 0.14 -0.8 84,057 - GFF5871 0.14 -1.5 84,057 - GFF5871 0.14 -0.7 84,057 - GFF5871 0.14 -1.1 84,360 + GFF5871 0.27 -0.8 84,360 + GFF5871 0.27 +0.6 84,361 - GFF5871 0.27 -0.8 84,438 + GFF5871 0.30 -0.7 84,438 + GFF5871 0.30 -1.2 84,438 + GFF5871 0.30 -1.3 84,438 + GFF5871 0.30 -0.6 84,438 + GFF5871 0.30 -1.5 84,438 + GFF5871 0.30 -1.3 84,439 - GFF5871 0.30 +0.8 84,439 - GFF5871 0.30 -1.1 84,439 - GFF5871 0.30 -0.7 84,439 - GFF5871 0.30 -0.8 84,439 - GFF5871 0.30 -1.0 84,450 + GFF5871 0.31 +1.5 84,450 + GFF5871 0.31 -2.3 84,450 + GFF5871 0.31 -1.2 84,451 - GFF5871 0.31 -0.0 84,451 - GFF5871 0.31 -0.2 84,451 - GFF5871 0.31 +0.8 84,451 - GFF5871 0.31 -1.2 84,451 - GFF5871 0.31 -1.6 84,502 - GFF5871 0.33 +0.5 84,552 + GFF5871 0.35 -1.0 84,552 + GFF5871 0.35 -0.5 84,552 + GFF5871 0.35 +0.4 84,552 + GFF5871 0.35 -1.0 84,552 + GFF5871 0.35 +2.8 84,552 + GFF5871 0.35 -1.2 84,552 + GFF5871 0.35 +0.1 84,552 + GFF5871 0.35 -0.9 84,552 + GFF5871 0.35 -0.2 84,552 + GFF5871 0.35 -0.7 84,553 - GFF5871 0.35 +0.1 84,553 - GFF5871 0.35 +0.7 84,662 + GFF5871 0.40 -1.9 84,664 - GFF5871 0.40 -2.0 84,738 + GFF5871 0.43 -0.3 84,738 + GFF5871 0.43 -1.2 84,738 + GFF5871 0.43 -1.6 84,738 + GFF5871 0.43 -2.5 84,738 + GFF5871 0.43 -1.5 84,738 + GFF5871 0.43 -0.8 84,738 + GFF5871 0.43 +1.2 84,738 + GFF5871 0.43 -0.3 84,738 + GFF5871 0.43 -1.5 84,739 - GFF5871 0.43 +0.1 84,739 - GFF5871 0.43 -0.3 84,739 - GFF5871 0.43 -0.1 84,739 - GFF5871 0.43 -0.2 84,739 - GFF5871 0.43 -0.9 84,739 - GFF5871 0.43 -1.1 84,739 - GFF5871 0.43 +0.3 84,739 - GFF5871 0.43 -1.0 84,864 + GFF5871 0.49 -1.0 84,864 + GFF5871 0.49 +0.4 84,864 + GFF5871 0.49 -2.1 84,865 - GFF5871 0.49 -1.3 85,022 + GFF5871 0.56 -1.2 85,022 + GFF5871 0.56 +0.4 85,023 - GFF5871 0.56 -1.5 85,023 - GFF5871 0.56 +0.2 85,086 + GFF5871 0.59 -0.8 85,086 + GFF5871 0.59 -0.9 85,086 + GFF5871 0.59 -0.6 85,086 + GFF5871 0.59 -1.0 85,086 + GFF5871 0.59 -1.5 85,086 + GFF5871 0.59 -1.5 85,087 - GFF5871 0.59 -0.6 85,087 - GFF5871 0.59 -0.5 85,087 - GFF5871 0.59 -1.0 85,087 - GFF5871 0.59 -1.4 85,203 + GFF5871 0.64 -2.1 85,203 + GFF5871 0.64 -0.2 85,203 + GFF5871 0.64 -1.8 85,236 + GFF5871 0.65 -0.4 85,237 - GFF5871 0.65 +0.6 85,237 - GFF5871 0.65 +0.5 85,317 + GFF5871 0.69 -0.5 86,080 + -1.1 86,080 + +0.0 86,080 + +0.1 86,081 - +0.5 86,081 - -0.4 86,081 - -0.4 86,081 - -0.9 86,081 - +0.5 86,081 - +0.9 86,081 - +0.3 86,081 - -1.1 86,081 - -0.8 86,083 + +1.0 86,083 + -0.1 86,083 + -0.0 86,084 - -0.3 86,269 + +0.6 86,269 + -2.1 86,269 + +0.0 86,270 - -1.3 86,270 - +0.3 86,270 - -0.0 86,270 - -0.1 86,270 - -0.2 86,270 - -0.2 86,270 - -0.1 86,270 - -0.4 86,562 + -0.5 86,562 + -1.3 86,562 + -0.9 86,562 + +0.1 86,563 - -0.2 86,563 - +0.0 86,563 - -0.3 86,563 - -1.6 86,580 + +0.3 86,580 + -0.2 86,581 - -0.5 86,581 - -0.3 86,581 - -0.8 86,581 - +1.2 86,581 - -0.3 86,581 - +0.8 86,613 + GFF5872 0.12 -1.0 86,613 + GFF5872 0.12 -0.4 86,613 + GFF5872 0.12 +0.2 86,614 - GFF5872 0.12 -0.3 86,614 - GFF5872 0.12 +0.0 86,614 - GFF5872 0.12 -0.0 86,614 - GFF5872 0.12 +0.1 86,614 - GFF5872 0.12 -0.6 86,614 - GFF5872 0.12 -0.4 86,614 - GFF5872 0.12 +0.6 86,616 + GFF5872 0.13 -0.5 86,616 + GFF5872 0.13 -0.4 86,616 + GFF5872 0.13 -0.8 86,616 + GFF5872 0.13 -0.7 86,616 + GFF5872 0.13 +0.2 86,617 - GFF5872 0.13 +0.8 86,617 - GFF5872 0.13 +0.8 86,617 - GFF5872 0.13 +0.2 86,628 + GFF5872 0.14 -0.0 86,628 + GFF5872 0.14 +0.0 86,688 + GFF5872 0.20 +1.2 86,689 - GFF5872 0.20 -0.3 86,689 - GFF5872 0.20 -0.5 86,689 - GFF5872 0.20 -0.7 86,689 - GFF5872 0.20 -0.4 86,689 - GFF5872 0.20 +1.1 86,778 + GFF5872 0.28 +2.8 86,779 - GFF5872 0.28 -0.5 86,805 + GFF5872 0.31 -0.6 86,805 + GFF5872 0.31 +0.9 86,805 + GFF5872 0.31 -0.1 86,805 + GFF5872 0.31 -2.3 86,805 + GFF5872 0.31 +0.1 86,805 + GFF5872 0.31 +0.6 86,805 + GFF5872 0.31 -0.8 86,806 - GFF5872 0.31 -0.2 86,806 - GFF5872 0.31 -0.1 86,806 - GFF5872 0.31 -1.0 86,806 - GFF5872 0.31 +2.1 86,806 - GFF5872 0.31 -0.9 86,943 + GFF5872 0.44 +0.2 86,943 + GFF5872 0.44 -0.2 86,943 + GFF5872 0.44 +0.8 86,943 + GFF5872 0.44 -0.3 86,943 + GFF5872 0.44 +0.0 86,944 - GFF5872 0.44 +0.6 86,944 - GFF5872 0.44 -0.0 86,944 - GFF5872 0.44 -2.3 86,944 - GFF5872 0.44 +0.2 86,944 - GFF5872 0.44 +0.0 86,944 - GFF5872 0.44 +0.2
Or see this region's nucleotide sequence