Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5787 and GFF5788 are separated by 12 nucleotides GFF5788 and GFF5789 are separated by 41 nucleotides GFF5789 and GFF5790 are separated by 16 nucleotides GFF5790 and GFF5791 overlap by 4 nucleotides
GFF5787 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 162,245 to 163,576
GFF5787
GFF5788 - FIG004556: membrane metalloprotease, at 163,589 to 164,260
GFF5788
GFF5789 - YciO protein, TsaC/YrdC paralog, at 164,302 to 164,937
GFF5789
GFF5790 - putative membrane protein, at 164,954 to 165,934
GFF5790
GFF5791 - FIG00031715: Predicted metal-dependent phosphoesterases (PHP family), at 165,931 to 166,788
GFF5791
Position (kb)
164
165 Strain fitness (log2 ratio)
-2
-1
0
1 at 163.591 kb on - strand at 163.635 kb on + strand at 163.635 kb on + strand at 163.635 kb on + strand at 163.636 kb on - strand at 163.636 kb on - strand at 163.636 kb on - strand at 163.636 kb on - strand at 163.668 kb on + strand, within GFF5788 at 163.668 kb on + strand, within GFF5788 at 163.668 kb on + strand, within GFF5788 at 163.668 kb on + strand, within GFF5788 at 163.669 kb on - strand, within GFF5788 at 163.669 kb on - strand, within GFF5788 at 164.061 kb on + strand, within GFF5788 at 164.061 kb on + strand, within GFF5788 at 164.062 kb on - strand, within GFF5788 at 164.135 kb on + strand, within GFF5788 at 164.136 kb on - strand, within GFF5788 at 164.136 kb on - strand, within GFF5788 at 164.136 kb on - strand, within GFF5788 at 164.136 kb on - strand, within GFF5788 at 164.172 kb on + strand, within GFF5788 at 164.173 kb on - strand, within GFF5788 at 164.173 kb on - strand, within GFF5788 at 164.173 kb on - strand, within GFF5788 at 164.173 kb on - strand, within GFF5788 at 164.173 kb on - strand, within GFF5788 at 164.223 kb on + strand at 164.223 kb on + strand at 164.223 kb on + strand at 164.223 kb on + strand at 164.223 kb on + strand at 164.223 kb on + strand at 164.223 kb on + strand at 164.224 kb on - strand at 164.224 kb on - strand at 164.224 kb on - strand at 164.224 kb on - strand at 164.224 kb on - strand at 164.225 kb on + strand at 164.225 kb on + strand at 164.225 kb on + strand at 164.225 kb on + strand at 164.225 kb on + strand at 164.225 kb on + strand at 164.226 kb on - strand at 164.231 kb on + strand at 164.231 kb on + strand at 164.241 kb on - strand at 164.255 kb on - strand at 164.289 kb on + strand at 164.289 kb on + strand at 164.289 kb on + strand at 164.290 kb on - strand at 164.290 kb on - strand at 164.290 kb on - strand at 164.290 kb on - strand at 164.290 kb on - strand at 164.648 kb on + strand, within GFF5789 at 164.648 kb on + strand, within GFF5789 at 164.648 kb on + strand, within GFF5789 at 164.648 kb on + strand, within GFF5789 at 164.648 kb on + strand, within GFF5789 at 164.649 kb on - strand, within GFF5789 at 164.649 kb on - strand, within GFF5789 at 164.681 kb on + strand, within GFF5789 at 164.682 kb on - strand, within GFF5789 at 164.706 kb on - strand, within GFF5789 at 164.706 kb on - strand, within GFF5789 at 164.825 kb on + strand, within GFF5789 at 164.826 kb on - strand, within GFF5789 at 164.927 kb on + strand at 164.928 kb on - strand at 164.944 kb on + strand at 164.944 kb on + strand at 164.945 kb on - strand at 165.080 kb on - strand, within GFF5790 at 165.117 kb on + strand, within GFF5790 at 165.117 kb on + strand, within GFF5790 at 165.123 kb on + strand, within GFF5790 at 165.123 kb on + strand, within GFF5790 at 165.124 kb on - strand, within GFF5790 at 165.156 kb on + strand, within GFF5790 at 165.157 kb on - strand, within GFF5790 at 165.164 kb on + strand, within GFF5790 at 165.165 kb on - strand, within GFF5790 at 165.297 kb on + strand, within GFF5790 at 165.298 kb on - strand, within GFF5790 at 165.298 kb on - strand, within GFF5790 at 165.570 kb on + strand, within GFF5790 at 165.570 kb on + strand, within GFF5790 at 165.570 kb on + strand, within GFF5790 at 165.570 kb on + strand, within GFF5790 at 165.570 kb on + strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.571 kb on - strand, within GFF5790 at 165.714 kb on + strand, within GFF5790 at 165.715 kb on - strand, within GFF5790
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 163,591 - -2.3 163,635 + +0.7 163,635 + +1.5 163,635 + -0.4 163,636 - +0.0 163,636 - -0.9 163,636 - -0.5 163,636 - -1.1 163,668 + GFF5788 0.12 +0.3 163,668 + GFF5788 0.12 -2.5 163,668 + GFF5788 0.12 -1.3 163,668 + GFF5788 0.12 +0.6 163,669 - GFF5788 0.12 -0.5 163,669 - GFF5788 0.12 +0.0 164,061 + GFF5788 0.70 -0.0 164,061 + GFF5788 0.70 -0.8 164,062 - GFF5788 0.70 -0.3 164,135 + GFF5788 0.81 -0.4 164,136 - GFF5788 0.81 +0.1 164,136 - GFF5788 0.81 +1.1 164,136 - GFF5788 0.81 +0.8 164,136 - GFF5788 0.81 -0.3 164,172 + GFF5788 0.87 +0.4 164,173 - GFF5788 0.87 -0.9 164,173 - GFF5788 0.87 -0.1 164,173 - GFF5788 0.87 +0.7 164,173 - GFF5788 0.87 +0.2 164,173 - GFF5788 0.87 +0.7 164,223 + -1.1 164,223 + -0.5 164,223 + +0.3 164,223 + -1.3 164,223 + -0.3 164,223 + +0.4 164,223 + +1.7 164,224 - +0.6 164,224 - +0.5 164,224 - +0.3 164,224 - -0.3 164,224 - -0.3 164,225 + +0.5 164,225 + +0.6 164,225 + +0.7 164,225 + -0.1 164,225 + +0.2 164,225 + +0.3 164,226 - -1.0 164,231 + -0.1 164,231 + -0.5 164,241 - -0.8 164,255 - +1.7 164,289 + +1.0 164,289 + -2.6 164,289 + -0.9 164,290 - +0.6 164,290 - -0.1 164,290 - -0.2 164,290 - -1.6 164,290 - -0.8 164,648 + GFF5789 0.54 +0.6 164,648 + GFF5789 0.54 -0.2 164,648 + GFF5789 0.54 +0.3 164,648 + GFF5789 0.54 +0.5 164,648 + GFF5789 0.54 +0.3 164,649 - GFF5789 0.55 -0.3 164,649 - GFF5789 0.55 +0.8 164,681 + GFF5789 0.60 -0.4 164,682 - GFF5789 0.60 +0.7 164,706 - GFF5789 0.64 +0.1 164,706 - GFF5789 0.64 -0.1 164,825 + GFF5789 0.82 +0.9 164,826 - GFF5789 0.82 -2.4 164,927 + +0.2 164,928 - +0.1 164,944 + -0.2 164,944 + -0.6 164,945 - -0.9 165,080 - GFF5790 0.13 +0.1 165,117 + GFF5790 0.17 +0.2 165,117 + GFF5790 0.17 -1.3 165,123 + GFF5790 0.17 +0.1 165,123 + GFF5790 0.17 -1.0 165,124 - GFF5790 0.17 -0.2 165,156 + GFF5790 0.21 -1.0 165,157 - GFF5790 0.21 +0.2 165,164 + GFF5790 0.21 -1.3 165,165 - GFF5790 0.22 -0.1 165,297 + GFF5790 0.35 +0.2 165,298 - GFF5790 0.35 -0.2 165,298 - GFF5790 0.35 -0.0 165,570 + GFF5790 0.63 +0.1 165,570 + GFF5790 0.63 +0.4 165,570 + GFF5790 0.63 -0.1 165,570 + GFF5790 0.63 -0.2 165,570 + GFF5790 0.63 -0.4 165,571 - GFF5790 0.63 -0.6 165,571 - GFF5790 0.63 +0.4 165,571 - GFF5790 0.63 +0.1 165,571 - GFF5790 0.63 +0.4 165,571 - GFF5790 0.63 -0.7 165,571 - GFF5790 0.63 +0.2 165,714 + GFF5790 0.77 +0.1 165,715 - GFF5790 0.78 -1.2
Or see this region's nucleotide sequence