Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5772 and GFF5773 are separated by 10 nucleotides GFF5773 and GFF5774 are separated by 34 nucleotides GFF5774 and GFF5775 are separated by 77 nucleotides
GFF5772 - Flagellar biosynthesis protein FlhF, at 145,163 to 147,700
GFF5772
GFF5773 - Flagellar synthesis regulator FleN, at 147,711 to 148,544
GFF5773
GFF5774 - RNA polymerase sigma factor for flagellar operon, at 148,579 to 149,313
GFF5774
GFF5775 - Glucoamylase (EC 3.2.1.3), at 149,391 to 151,298
GFF5775
Position (kb) 148
149
150 Strain fitness (log2 ratio) -1
0
1 at 148.216 kb on - strand, within GFF5773 at 148.744 kb on + strand, within GFF5774 at 149.002 kb on + strand, within GFF5774 at 149.188 kb on + strand, within GFF5774 at 149.189 kb on - strand, within GFF5774 at 149.191 kb on + strand, within GFF5774 at 149.191 kb on + strand, within GFF5774 at 149.192 kb on - strand, within GFF5774 at 149.319 kb on + strand at 149.646 kb on + strand, within GFF5775 at 149.647 kb on - strand, within GFF5775 at 149.647 kb on - strand, within GFF5775 at 149.647 kb on - strand, within GFF5775 at 150.147 kb on + strand, within GFF5775 at 150.147 kb on + strand, within GFF5775 at 150.147 kb on + strand, within GFF5775 at 150.147 kb on + strand, within GFF5775 at 150.147 kb on + strand, within GFF5775 at 150.226 kb on - strand, within GFF5775 at 150.265 kb on - strand, within GFF5775 at 150.286 kb on - strand, within GFF5775 at 150.286 kb on - strand, within GFF5775
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 148,216 - GFF5773 0.61 -0.2 148,744 + GFF5774 0.22 -0.9 149,002 + GFF5774 0.58 -0.7 149,188 + GFF5774 0.83 -1.4 149,189 - GFF5774 0.83 -0.2 149,191 + GFF5774 0.83 -0.4 149,191 + GFF5774 0.83 -0.4 149,192 - GFF5774 0.83 -0.1 149,319 + +0.1 149,646 + GFF5775 0.13 -0.6 149,647 - GFF5775 0.13 -0.3 149,647 - GFF5775 0.13 -0.0 149,647 - GFF5775 0.13 -0.2 150,147 + GFF5775 0.40 -1.4 150,147 + GFF5775 0.40 -0.2 150,147 + GFF5775 0.40 -0.8 150,147 + GFF5775 0.40 +1.1 150,147 + GFF5775 0.40 +1.0 150,226 - GFF5775 0.44 -0.4 150,265 - GFF5775 0.46 -1.2 150,286 - GFF5775 0.47 -1.4 150,286 - GFF5775 0.47 -1.2
Or see this region's nucleotide sequence