Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5686 and GFF5687 overlap by 8 nucleotides GFF5687 and GFF5688 are separated by 115 nucleotides GFF5688 and GFF5689 overlap by 25 nucleotides GFF5689 and GFF5690 overlap by 4 nucleotides
GFF5686 - FIG01027227: hypothetical protein, at 62,361 to 62,759
GFF5686
GFF5687 - FIG01027591: hypothetical protein, at 62,752 to 63,306
GFF5687
GFF5688 - Transcriptional regulator, LysR family, at 63,422 to 64,345
GFF5688
GFF5689 - Oxidoreductase, 2OG-Fe(II) oxygenase family, at 64,321 to 65,322
GFF5689
GFF5690 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 65,319 to 66,062
GFF5690
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 62.449 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.810 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.033 kb on - strand, within GFF5687 at 63.033 kb on - strand, within GFF5687 at 63.359 kb on + strand at 63.359 kb on + strand at 63.359 kb on + strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.534 kb on - strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.377 kb on - strand at 64.377 kb on - strand at 64.377 kb on - strand at 64.572 kb on - strand, within GFF5689 at 64.572 kb on - strand, within GFF5689 at 64.572 kb on - strand, within GFF5689 at 64.778 kb on + strand, within GFF5689 at 64.779 kb on - strand, within GFF5689 at 64.817 kb on + strand, within GFF5689 at 64.818 kb on - strand, within GFF5689 at 64.833 kb on - strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.908 kb on - strand, within GFF5689 at 64.908 kb on - strand, within GFF5689 at 65.018 kb on + strand, within GFF5689 at 65.018 kb on + strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 62,449 + GFF5686 0.22 +0.3 62,710 + GFF5686 0.87 +0.0 62,710 + GFF5686 0.87 -1.1 62,711 - GFF5686 0.88 +2.3 62,711 - GFF5686 0.88 -0.5 62,711 - GFF5686 0.88 -0.6 62,810 + GFF5687 0.10 -0.8 63,032 + GFF5687 0.50 -0.8 63,032 + GFF5687 0.50 -0.7 63,032 + GFF5687 0.50 -0.2 63,032 + GFF5687 0.50 +0.3 63,033 - GFF5687 0.51 +0.2 63,033 - GFF5687 0.51 +1.1 63,359 + +0.8 63,359 + -0.9 63,359 + -0.2 63,360 - +0.3 63,360 - -0.7 63,360 - +0.1 63,360 - -0.5 63,360 - -0.3 63,534 - GFF5688 0.12 -1.2 63,728 + GFF5688 0.33 -1.3 63,728 + GFF5688 0.33 -0.9 63,728 + GFF5688 0.33 -1.4 63,729 - GFF5688 0.33 -1.3 63,729 - GFF5688 0.33 +0.6 63,729 - GFF5688 0.33 -0.7 63,729 - GFF5688 0.33 +0.7 64,376 + -0.6 64,376 + -0.5 64,376 + -0.8 64,376 + +1.7 64,376 + +0.1 64,377 - -0.3 64,377 - +1.7 64,377 - -2.1 64,572 - GFF5689 0.25 -0.3 64,572 - GFF5689 0.25 -0.5 64,572 - GFF5689 0.25 -0.4 64,778 + GFF5689 0.46 -0.1 64,779 - GFF5689 0.46 -1.0 64,817 + GFF5689 0.50 +0.3 64,818 - GFF5689 0.50 -0.6 64,833 - GFF5689 0.51 -0.0 64,907 + GFF5689 0.58 +0.9 64,907 + GFF5689 0.58 +1.7 64,907 + GFF5689 0.58 -0.7 64,908 - GFF5689 0.59 -0.3 64,908 - GFF5689 0.59 -0.5 65,018 + GFF5689 0.70 -0.2 65,018 + GFF5689 0.70 -1.1 65,019 - GFF5689 0.70 -1.4 65,019 - GFF5689 0.70 -0.7 65,019 - GFF5689 0.70 -0.8 65,207 + GFF5689 0.88 -3.1 65,207 + GFF5689 0.88 -0.2 65,207 + GFF5689 0.88 +1.7 65,207 + GFF5689 0.88 +0.3 65,208 - GFF5689 0.89 -0.7 65,208 - GFF5689 0.89 -0.8 65,208 - GFF5689 0.89 -0.4 65,208 - GFF5689 0.89 +0.7 65,208 - GFF5689 0.89 -1.6
Or see this region's nucleotide sequence