Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5684 and GFF5685 are separated by 13 nucleotides GFF5685 and GFF5686 are separated by 25 nucleotides GFF5686 and GFF5687 overlap by 8 nucleotides
GFF5684 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 60,346 to 61,515
GFF5684
GFF5685 - Gluconate 5-dehydrogenase (EC 1.1.1.69), at 61,529 to 62,335
GFF5685
GFF5686 - FIG01027227: hypothetical protein, at 62,361 to 62,759
GFF5686
GFF5687 - FIG01027591: hypothetical protein, at 62,752 to 63,306
GFF5687
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1
2 at 60.624 kb on - strand, within GFF5684 at 60.624 kb on - strand, within GFF5684 at 60.771 kb on - strand, within GFF5684 at 60.771 kb on - strand, within GFF5684 at 60.971 kb on + strand, within GFF5684 at 60.972 kb on - strand, within GFF5684 at 61.053 kb on - strand, within GFF5684 at 61.118 kb on + strand, within GFF5684 at 61.118 kb on + strand, within GFF5684 at 61.118 kb on + strand, within GFF5684 at 61.118 kb on + strand, within GFF5684 at 61.118 kb on + strand, within GFF5684 at 61.119 kb on - strand, within GFF5684 at 61.719 kb on + strand, within GFF5685 at 61.719 kb on + strand, within GFF5685 at 61.719 kb on + strand, within GFF5685 at 61.720 kb on - strand, within GFF5685 at 61.720 kb on - strand, within GFF5685 at 61.720 kb on - strand, within GFF5685 at 61.720 kb on - strand, within GFF5685 at 61.720 kb on - strand, within GFF5685 at 61.722 kb on + strand, within GFF5685 at 61.723 kb on - strand, within GFF5685 at 61.723 kb on - strand, within GFF5685 at 61.830 kb on + strand, within GFF5685 at 61.830 kb on + strand, within GFF5685 at 61.831 kb on - strand, within GFF5685 at 61.872 kb on + strand, within GFF5685 at 61.872 kb on + strand, within GFF5685 at 62.449 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.810 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.033 kb on - strand, within GFF5687 at 63.033 kb on - strand, within GFF5687
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 60,624 - GFF5684 0.24 +0.6 60,624 - GFF5684 0.24 -0.8 60,771 - GFF5684 0.36 +0.7 60,771 - GFF5684 0.36 -0.1 60,971 + GFF5684 0.53 -0.1 60,972 - GFF5684 0.54 +0.5 61,053 - GFF5684 0.60 +0.2 61,118 + GFF5684 0.66 +1.3 61,118 + GFF5684 0.66 +0.5 61,118 + GFF5684 0.66 -0.4 61,118 + GFF5684 0.66 +0.1 61,118 + GFF5684 0.66 -0.2 61,119 - GFF5684 0.66 -0.1 61,719 + GFF5685 0.24 -0.9 61,719 + GFF5685 0.24 +0.5 61,719 + GFF5685 0.24 +0.1 61,720 - GFF5685 0.24 -1.4 61,720 - GFF5685 0.24 -1.2 61,720 - GFF5685 0.24 +0.3 61,720 - GFF5685 0.24 +1.7 61,720 - GFF5685 0.24 -0.4 61,722 + GFF5685 0.24 +0.8 61,723 - GFF5685 0.24 -0.9 61,723 - GFF5685 0.24 +1.2 61,830 + GFF5685 0.37 +1.7 61,830 + GFF5685 0.37 +1.1 61,831 - GFF5685 0.37 -0.3 61,872 + GFF5685 0.43 -0.4 61,872 + GFF5685 0.43 -0.6 62,449 + GFF5686 0.22 +0.3 62,710 + GFF5686 0.87 +0.0 62,710 + GFF5686 0.87 -1.1 62,711 - GFF5686 0.88 +2.3 62,711 - GFF5686 0.88 -0.5 62,711 - GFF5686 0.88 -0.6 62,810 + GFF5687 0.10 -0.8 63,032 + GFF5687 0.50 -0.8 63,032 + GFF5687 0.50 -0.7 63,032 + GFF5687 0.50 -0.2 63,032 + GFF5687 0.50 +0.3 63,033 - GFF5687 0.51 +0.2 63,033 - GFF5687 0.51 +1.1
Or see this region's nucleotide sequence