Strain Fitness in Variovorax sp. SCN45 around GFF5684

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5683 and GFF5684 overlap by 4 nucleotidesGFF5684 and GFF5685 are separated by 13 nucleotidesGFF5685 and GFF5686 are separated by 25 nucleotides GFF5683 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 58,760 to 60,349 GFF5683 GFF5684 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 60,346 to 61,515 GFF5684 GFF5685 - Gluconate 5-dehydrogenase (EC 1.1.1.69), at 61,529 to 62,335 GFF5685 GFF5686 - FIG01027227: hypothetical protein, at 62,361 to 62,759 GFF5686 Position (kb) 60 61 62Strain fitness (log2 ratio) -2 -1 0 1at 59.415 kb on + strand, within GFF5683at 59.415 kb on + strand, within GFF5683at 59.415 kb on + strand, within GFF5683at 59.416 kb on - strand, within GFF5683at 59.416 kb on - strand, within GFF5683at 59.470 kb on - strand, within GFF5683at 59.470 kb on - strand, within GFF5683at 59.487 kb on + strand, within GFF5683at 59.487 kb on + strand, within GFF5683at 59.487 kb on + strand, within GFF5683at 59.487 kb on + strand, within GFF5683at 59.487 kb on + strand, within GFF5683at 59.488 kb on - strand, within GFF5683at 59.488 kb on - strand, within GFF5683at 59.496 kb on + strand, within GFF5683at 59.496 kb on + strand, within GFF5683at 59.496 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.838 kb on + strand, within GFF5683at 59.839 kb on - strand, within GFF5683at 59.839 kb on - strand, within GFF5683at 59.839 kb on - strand, within GFF5683at 59.844 kb on + strand, within GFF5683at 59.844 kb on + strand, within GFF5683at 59.844 kb on + strand, within GFF5683at 59.844 kb on + strand, within GFF5683at 59.844 kb on + strand, within GFF5683at 59.845 kb on - strand, within GFF5683at 59.845 kb on - strand, within GFF5683at 59.919 kb on + strand, within GFF5683at 59.920 kb on - strand, within GFF5683at 60.057 kb on + strand, within GFF5683at 60.058 kb on - strand, within GFF5683at 60.058 kb on - strand, within GFF5683at 60.371 kb on + strandat 60.371 kb on + strandat 60.371 kb on + strandat 60.371 kb on + strandat 60.372 kb on - strandat 60.372 kb on - strandat 60.372 kb on - strandat 60.624 kb on - strand, within GFF5684at 60.624 kb on - strand, within GFF5684at 60.771 kb on - strand, within GFF5684at 60.771 kb on - strand, within GFF5684at 60.971 kb on + strand, within GFF5684at 60.972 kb on - strand, within GFF5684at 61.053 kb on - strand, within GFF5684at 61.118 kb on + strand, within GFF5684at 61.118 kb on + strand, within GFF5684at 61.118 kb on + strand, within GFF5684at 61.118 kb on + strand, within GFF5684at 61.118 kb on + strand, within GFF5684at 61.119 kb on - strand, within GFF5684at 61.719 kb on + strand, within GFF5685at 61.719 kb on + strand, within GFF5685at 61.719 kb on + strand, within GFF5685at 61.720 kb on - strand, within GFF5685at 61.720 kb on - strand, within GFF5685at 61.720 kb on - strand, within GFF5685at 61.720 kb on - strand, within GFF5685at 61.720 kb on - strand, within GFF5685at 61.722 kb on + strand, within GFF5685at 61.723 kb on - strand, within GFF5685at 61.723 kb on - strand, within GFF5685at 61.830 kb on + strand, within GFF5685at 61.830 kb on + strand, within GFF5685at 61.831 kb on - strand, within GFF5685at 61.872 kb on + strand, within GFF5685at 61.872 kb on + strand, within GFF5685at 62.449 kb on + strand, within GFF5686

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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59,415 + GFF5683 0.41 +1.7
59,415 + GFF5683 0.41 +0.2
59,415 + GFF5683 0.41 +0.1
59,416 - GFF5683 0.41 +0.2
59,416 - GFF5683 0.41 -0.2
59,470 - GFF5683 0.45 -0.7
59,470 - GFF5683 0.45 +0.2
59,487 + GFF5683 0.46 -0.8
59,487 + GFF5683 0.46 -0.3
59,487 + GFF5683 0.46 -0.2
59,487 + GFF5683 0.46 -0.9
59,487 + GFF5683 0.46 +0.5
59,488 - GFF5683 0.46 +1.7
59,488 - GFF5683 0.46 -0.4
59,496 + GFF5683 0.46 +0.3
59,496 + GFF5683 0.46 -1.1
59,496 + GFF5683 0.46 -0.3
59,838 + GFF5683 0.68 -1.4
59,838 + GFF5683 0.68 +0.1
59,838 + GFF5683 0.68 -0.5
59,838 + GFF5683 0.68 -1.0
59,838 + GFF5683 0.68 -2.3
59,838 + GFF5683 0.68 -0.3
59,839 - GFF5683 0.68 +0.1
59,839 - GFF5683 0.68 +1.3
59,839 - GFF5683 0.68 -0.4
59,844 + GFF5683 0.68 -0.5
59,844 + GFF5683 0.68 -0.6
59,844 + GFF5683 0.68 -0.5
59,844 + GFF5683 0.68 -0.3
59,844 + GFF5683 0.68 -0.5
59,845 - GFF5683 0.68 -1.1
59,845 - GFF5683 0.68 -2.7
59,919 + GFF5683 0.73 -1.1
59,920 - GFF5683 0.73 -1.3
60,057 + GFF5683 0.82 -0.7
60,058 - GFF5683 0.82 +0.7
60,058 - GFF5683 0.82 +1.0
60,371 + +0.6
60,371 + +0.9
60,371 + -0.4
60,371 + -0.6
60,372 - +0.1
60,372 - +0.1
60,372 - -0.4
60,624 - GFF5684 0.24 +0.6
60,624 - GFF5684 0.24 -0.8
60,771 - GFF5684 0.36 +0.7
60,771 - GFF5684 0.36 -0.1
60,971 + GFF5684 0.53 -0.1
60,972 - GFF5684 0.54 +0.5
61,053 - GFF5684 0.60 +0.2
61,118 + GFF5684 0.66 +1.3
61,118 + GFF5684 0.66 +0.5
61,118 + GFF5684 0.66 -0.4
61,118 + GFF5684 0.66 +0.1
61,118 + GFF5684 0.66 -0.2
61,119 - GFF5684 0.66 -0.1
61,719 + GFF5685 0.24 -0.9
61,719 + GFF5685 0.24 +0.5
61,719 + GFF5685 0.24 +0.1
61,720 - GFF5685 0.24 -1.4
61,720 - GFF5685 0.24 -1.2
61,720 - GFF5685 0.24 +0.3
61,720 - GFF5685 0.24 +1.7
61,720 - GFF5685 0.24 -0.4
61,722 + GFF5685 0.24 +0.8
61,723 - GFF5685 0.24 -0.9
61,723 - GFF5685 0.24 +1.2
61,830 + GFF5685 0.37 +1.7
61,830 + GFF5685 0.37 +1.1
61,831 - GFF5685 0.37 -0.3
61,872 + GFF5685 0.43 -0.4
61,872 + GFF5685 0.43 -0.6
62,449 + GFF5686 0.22 +0.3

Or see this region's nucleotide sequence