Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5364 and GFF5365 are separated by 135 nucleotides GFF5365 and GFF5366 are separated by 296 nucleotides
GFF5364 - Twin-arginine translocation protein TatC, at 236,754 to 237,566
GFF5364
GFF5365 - Outer membrane stress sensor protease DegS, at 237,702 to 238,862
GFF5365
GFF5366 - L-Proline/Glycine betaine transporter ProP, at 239,159 to 240,511
GFF5366
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1 at 237.625 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 237.703 kb on + strand at 238.129 kb on + strand, within GFF5365 at 238.129 kb on + strand, within GFF5365 at 238.130 kb on - strand, within GFF5365 at 238.130 kb on - strand, within GFF5365 at 238.130 kb on - strand, within GFF5365 at 238.516 kb on + strand, within GFF5365 at 238.516 kb on + strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.517 kb on - strand, within GFF5365 at 238.807 kb on + strand at 238.807 kb on + strand at 238.808 kb on - strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.853 kb on + strand at 238.854 kb on - strand at 238.854 kb on - strand at 238.854 kb on - strand at 238.854 kb on - strand at 238.854 kb on - strand at 238.944 kb on + strand at 238.945 kb on - strand at 238.945 kb on - strand at 238.945 kb on - strand at 238.945 kb on - strand at 238.945 kb on - strand at 239.005 kb on - strand at 239.005 kb on - strand at 239.008 kb on - strand at 239.008 kb on - strand at 239.008 kb on - strand at 239.008 kb on - strand at 239.008 kb on - strand at 239.145 kb on + strand at 239.146 kb on - strand at 239.228 kb on + strand at 239.228 kb on + strand at 239.229 kb on - strand at 239.274 kb on - strand at 239.274 kb on - strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.288 kb on + strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.289 kb on - strand at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.729 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.808 kb on - strand, within GFF5366 at 239.808 kb on - strand, within GFF5366 at 239.808 kb on - strand, within GFF5366
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 237,625 + -0.8 237,703 + -0.4 237,703 + -0.4 237,703 + -2.4 237,703 + +0.7 237,703 + -0.3 237,703 + -0.2 237,703 + -0.3 237,703 + -0.5 238,129 + GFF5365 0.37 +0.8 238,129 + GFF5365 0.37 -0.3 238,130 - GFF5365 0.37 +1.7 238,130 - GFF5365 0.37 +0.7 238,130 - GFF5365 0.37 -1.4 238,516 + GFF5365 0.70 +0.5 238,516 + GFF5365 0.70 +0.4 238,517 - GFF5365 0.70 -0.0 238,517 - GFF5365 0.70 -0.4 238,517 - GFF5365 0.70 -0.2 238,517 - GFF5365 0.70 +0.6 238,517 - GFF5365 0.70 +0.4 238,517 - GFF5365 0.70 -0.2 238,517 - GFF5365 0.70 -0.0 238,807 + -0.4 238,807 + -0.3 238,808 - -0.7 238,853 + -0.4 238,853 + +1.2 238,853 + +0.6 238,853 + +0.1 238,853 + -0.2 238,853 + +0.7 238,853 + -0.7 238,854 - +1.5 238,854 - -0.8 238,854 - -0.1 238,854 - -0.3 238,854 - -0.6 238,944 + -2.2 238,945 - +0.3 238,945 - +1.7 238,945 - -0.1 238,945 - -0.3 238,945 - +0.1 239,005 - -0.8 239,005 - -0.4 239,008 - -0.2 239,008 - -0.1 239,008 - -0.1 239,008 - -0.1 239,008 - +0.7 239,145 + -0.4 239,146 - -0.8 239,228 + -0.2 239,228 + -0.9 239,229 - +0.4 239,274 - -0.4 239,274 - +1.1 239,288 + -0.2 239,288 + +0.6 239,288 + +0.7 239,288 + -0.2 239,288 + +1.7 239,288 + -0.4 239,288 + +0.1 239,288 + -0.4 239,288 + +0.2 239,288 + +0.7 239,288 + -0.4 239,288 + -0.7 239,289 - +0.8 239,289 - -0.4 239,289 - -2.3 239,289 - -0.9 239,289 - -0.5 239,289 - -0.4 239,289 - -1.3 239,289 - +0.5 239,289 - -0.9 239,289 - -0.9 239,516 + GFF5366 0.26 +1.4 239,516 + GFF5366 0.26 +0.0 239,516 + GFF5366 0.26 -0.9 239,516 + GFF5366 0.26 +0.4 239,516 + GFF5366 0.26 +0.1 239,517 - GFF5366 0.26 -0.7 239,517 - GFF5366 0.26 -0.0 239,517 - GFF5366 0.26 -0.1 239,517 - GFF5366 0.26 -0.2 239,729 + GFF5366 0.42 +1.3 239,807 + GFF5366 0.48 -0.6 239,807 + GFF5366 0.48 -0.7 239,807 + GFF5366 0.48 +0.1 239,807 + GFF5366 0.48 -0.1 239,807 + GFF5366 0.48 +0.3 239,807 + GFF5366 0.48 -0.3 239,807 + GFF5366 0.48 -0.9 239,808 - GFF5366 0.48 -1.7 239,808 - GFF5366 0.48 -0.1 239,808 - GFF5366 0.48 +0.7
Or see this region's nucleotide sequence