Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4969 and GFF4970 are separated by 24 nucleotides GFF4970 and GFF4971 are separated by 24 nucleotides GFF4971 and GFF4972 overlap by 8 nucleotides GFF4972 and GFF4973 overlap by 4 nucleotides
GFF4969 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,219 to 70,962
GFF4969
GFF4970 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,987 to 71,736
GFF4970
GFF4971 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 71,761 to 72,480
GFF4971
GFF4972 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 72,473 to 73,237
GFF4972
GFF4973 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 73,234 to 74,217
GFF4973
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.844 kb on + strand, within GFF4969 at 70.844 kb on + strand, within GFF4969 at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.040 kb on + strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.124 kb on - strand, within GFF4970 at 71.124 kb on - strand, within GFF4970 at 71.277 kb on - strand, within GFF4970 at 71.277 kb on - strand, within GFF4970 at 71.853 kb on - strand, within GFF4971 at 71.853 kb on - strand, within GFF4971 at 71.858 kb on + strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.438 kb on - strand at 72.501 kb on + strand at 72.501 kb on + strand at 72.502 kb on - strand at 72.503 kb on - strand at 73.071 kb on + strand, within GFF4972 at 73.071 kb on + strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.353 kb on - strand, within GFF4973
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 70,844 + GFF4969 0.84 +1.0 70,844 + GFF4969 0.84 +0.3 71,036 + -1.3 71,036 + -2.2 71,036 + -0.7 71,036 + +0.1 71,036 + -0.1 71,037 - -2.0 71,037 - -0.6 71,037 - +0.3 71,040 + -0.3 71,041 - -0.7 71,041 - -0.4 71,041 - -1.9 71,121 + GFF4970 0.18 +0.1 71,121 + GFF4970 0.18 +0.5 71,121 + GFF4970 0.18 -1.0 71,122 - GFF4970 0.18 +0.6 71,122 - GFF4970 0.18 +0.1 71,122 - GFF4970 0.18 +0.0 71,123 + GFF4970 0.18 -0.1 71,123 + GFF4970 0.18 +0.2 71,123 + GFF4970 0.18 +0.0 71,123 + GFF4970 0.18 -0.7 71,124 - GFF4970 0.18 +0.3 71,124 - GFF4970 0.18 -0.4 71,277 - GFF4970 0.39 -0.7 71,277 - GFF4970 0.39 -0.3 71,853 - GFF4971 0.13 +0.3 71,853 - GFF4971 0.13 +0.4 71,858 + GFF4971 0.13 -0.4 71,859 - GFF4971 0.14 -0.4 71,859 - GFF4971 0.14 -0.4 72,117 - GFF4971 0.49 +0.6 72,117 - GFF4971 0.49 +1.9 72,165 - GFF4971 0.56 +1.0 72,165 - GFF4971 0.56 +0.5 72,165 - GFF4971 0.56 +0.7 72,438 - +0.6 72,501 + -0.0 72,501 + -0.6 72,502 - +1.5 72,503 - -1.1 73,071 + GFF4972 0.78 -0.6 73,071 + GFF4972 0.78 +0.4 73,072 - GFF4972 0.78 -0.5 73,072 - GFF4972 0.78 -0.4 73,352 + GFF4973 0.12 +0.9 73,352 + GFF4973 0.12 -1.0 73,352 + GFF4973 0.12 +0.2 73,353 - GFF4973 0.12 +0.2
Or see this region's nucleotide sequence