Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4773 and GFF4774 are separated by 1 nucleotides GFF4774 and GFF4775 are separated by 64 nucleotides
GFF4773 - Broad-specificity amino acid ABC transporter, ATP-binding protein 1, at 141,697 to 142,467
GFF4773
GFF4774 - Broad-specificity amino acid ABC transporter, ATP-binding protein 2, at 142,469 to 143,176
GFF4774
GFF4775 - Broad-specificity amino acid ABC transporter, substrate-binding protein, at 143,241 to 144,437
GFF4775
Position (kb) 142
143
144 Strain fitness (log2 ratio) -2
-1
0
1
2 at 141.487 kb on + strand at 141.487 kb on + strand at 141.487 kb on + strand at 141.487 kb on + strand at 141.487 kb on + strand at 141.487 kb on + strand at 141.488 kb on - strand at 141.488 kb on - strand at 141.488 kb on - strand at 141.488 kb on - strand at 141.488 kb on - strand at 141.488 kb on - strand at 141.573 kb on + strand at 141.574 kb on - strand at 141.589 kb on + strand at 142.033 kb on + strand, within GFF4773 at 142.033 kb on + strand, within GFF4773 at 142.033 kb on + strand, within GFF4773 at 142.034 kb on - strand, within GFF4773 at 142.034 kb on - strand, within GFF4773 at 142.353 kb on - strand, within GFF4773 at 142.388 kb on - strand, within GFF4773 at 142.388 kb on - strand, within GFF4773 at 142.426 kb on + strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.823 kb on + strand, within GFF4774 at 142.823 kb on + strand, within GFF4774 at 142.823 kb on + strand, within GFF4774 at 142.823 kb on + strand, within GFF4774 at 142.823 kb on + strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 142.824 kb on - strand, within GFF4774 at 143.024 kb on + strand, within GFF4774 at 143.024 kb on + strand, within GFF4774 at 143.024 kb on + strand, within GFF4774 at 143.024 kb on + strand, within GFF4774 at 143.025 kb on - strand, within GFF4774 at 143.025 kb on - strand, within GFF4774 at 143.085 kb on - strand, within GFF4774 at 143.358 kb on + strand at 143.358 kb on + strand at 143.359 kb on - strand at 143.601 kb on + strand, within GFF4775 at 143.601 kb on + strand, within GFF4775 at 143.602 kb on - strand, within GFF4775 at 143.602 kb on - strand, within GFF4775 at 143.656 kb on - strand, within GFF4775 at 143.656 kb on - strand, within GFF4775 at 143.757 kb on + strand, within GFF4775 at 143.757 kb on + strand, within GFF4775 at 144.069 kb on + strand, within GFF4775 at 144.069 kb on + strand, within GFF4775 at 144.160 kb on - strand, within GFF4775
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 141,487 + -0.1 141,487 + -0.5 141,487 + +2.0 141,487 + +0.2 141,487 + -1.2 141,487 + -0.3 141,488 - +0.2 141,488 - -0.3 141,488 - -0.5 141,488 - -1.2 141,488 - -0.8 141,488 - +0.6 141,573 + +0.5 141,574 - +1.0 141,589 + +0.4 142,033 + GFF4773 0.44 -0.2 142,033 + GFF4773 0.44 -0.3 142,033 + GFF4773 0.44 -0.4 142,034 - GFF4773 0.44 +0.2 142,034 - GFF4773 0.44 +0.2 142,353 - GFF4773 0.85 +0.2 142,388 - GFF4773 0.90 -0.6 142,388 - GFF4773 0.90 -0.9 142,426 + -1.2 142,427 - +0.3 142,427 - -0.8 142,427 - -2.3 142,427 - +0.4 142,823 + GFF4774 0.50 -0.2 142,823 + GFF4774 0.50 +0.2 142,823 + GFF4774 0.50 -0.0 142,823 + GFF4774 0.50 +1.3 142,823 + GFF4774 0.50 -0.3 142,824 - GFF4774 0.50 -0.5 142,824 - GFF4774 0.50 +0.4 142,824 - GFF4774 0.50 -0.2 142,824 - GFF4774 0.50 +0.0 142,824 - GFF4774 0.50 -0.0 142,824 - GFF4774 0.50 +1.0 142,824 - GFF4774 0.50 -0.6 143,024 + GFF4774 0.78 -0.2 143,024 + GFF4774 0.78 -0.2 143,024 + GFF4774 0.78 +0.8 143,024 + GFF4774 0.78 +0.5 143,025 - GFF4774 0.79 -0.6 143,025 - GFF4774 0.79 -0.3 143,085 - GFF4774 0.87 -0.3 143,358 + +0.5 143,358 + -0.3 143,359 - +0.1 143,601 + GFF4775 0.30 -0.1 143,601 + GFF4775 0.30 -1.6 143,602 - GFF4775 0.30 -1.9 143,602 - GFF4775 0.30 +0.8 143,656 - GFF4775 0.35 +1.0 143,656 - GFF4775 0.35 -0.1 143,757 + GFF4775 0.43 +0.5 143,757 + GFF4775 0.43 -0.9 144,069 + GFF4775 0.69 +0.4 144,069 + GFF4775 0.69 -0.4 144,160 - GFF4775 0.77 -0.9
Or see this region's nucleotide sequence