Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4549 and GFF4550 are separated by 36 nucleotides GFF4550 and GFF4551 overlap by 1 nucleotides GFF4551 and GFF4552 are separated by 8 nucleotides
GFF4549 - Cysteine synthase (EC 2.5.1.47), at 186,142 to 187,062
GFF4549
GFF4550 - NAD(FAD)-utilizing dehydrogenase, sll0175 homolog, at 187,099 to 188,784
GFF4550
GFF4551 - Putative preQ0 transporter YhhQ, at 188,784 to 189,353
GFF4551
GFF4552 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 189,362 to 189,991
GFF4552
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 186.400 kb on + strand, within GFF4549 at 186.400 kb on + strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.463 kb on + strand, within GFF4549 at 187.012 kb on + strand at 187.012 kb on + strand at 187.012 kb on + strand at 187.013 kb on - strand at 187.013 kb on - strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.117 kb on - strand at 187.117 kb on - strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.129 kb on + strand at 187.129 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.396 kb on + strand, within GFF4550 at 187.396 kb on + strand, within GFF4550 at 187.397 kb on - strand, within GFF4550 at 187.397 kb on - strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.646 kb on - strand, within GFF4550 at 187.646 kb on - strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.848 kb on + strand, within GFF4550 at 188.104 kb on + strand, within GFF4550 at 188.104 kb on + strand, within GFF4550 at 188.104 kb on + strand, within GFF4550 at 188.104 kb on + strand, within GFF4550 at 188.104 kb on + strand, within GFF4550 at 188.105 kb on - strand, within GFF4550 at 188.105 kb on - strand, within GFF4550 at 188.105 kb on - strand, within GFF4550 at 188.105 kb on - strand, within GFF4550 at 188.143 kb on + strand, within GFF4550 at 188.143 kb on + strand, within GFF4550 at 188.143 kb on + strand, within GFF4550 at 188.143 kb on + strand, within GFF4550 at 188.143 kb on + strand, within GFF4550 at 188.144 kb on - strand, within GFF4550 at 188.144 kb on - strand, within GFF4550 at 188.144 kb on - strand, within GFF4550 at 188.144 kb on - strand, within GFF4550 at 188.144 kb on - strand, within GFF4550 at 188.227 kb on + strand, within GFF4550 at 188.227 kb on + strand, within GFF4550 at 188.228 kb on - strand, within GFF4550 at 188.288 kb on - strand, within GFF4550 at 188.365 kb on + strand, within GFF4550 at 188.443 kb on + strand, within GFF4550 at 188.443 kb on + strand, within GFF4550 at 188.443 kb on + strand, within GFF4550 at 188.443 kb on + strand, within GFF4550 at 188.443 kb on + strand, within GFF4550 at 188.444 kb on - strand, within GFF4550 at 188.444 kb on - strand, within GFF4550 at 188.444 kb on - strand, within GFF4550 at 188.444 kb on - strand, within GFF4550 at 188.491 kb on + strand, within GFF4550 at 188.491 kb on + strand, within GFF4550 at 188.491 kb on + strand, within GFF4550 at 188.492 kb on - strand, within GFF4550 at 188.677 kb on + strand at 188.678 kb on - strand at 188.928 kb on + strand, within GFF4551 at 188.928 kb on + strand, within GFF4551 at 188.928 kb on + strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.929 kb on - strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.940 kb on + strand, within GFF4551 at 188.941 kb on - strand, within GFF4551 at 188.941 kb on - strand, within GFF4551 at 188.941 kb on - strand, within GFF4551 at 189.392 kb on + strand at 189.392 kb on + strand at 189.392 kb on + strand at 189.392 kb on + strand at 189.392 kb on + strand at 189.392 kb on + strand at 189.392 kb on + strand at 189.393 kb on - strand at 189.393 kb on - strand at 189.393 kb on - strand at 189.393 kb on - strand at 189.393 kb on - strand at 189.393 kb on - strand at 189.509 kb on + strand, within GFF4552 at 189.533 kb on + strand, within GFF4552 at 189.533 kb on + strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552 at 189.534 kb on - strand, within GFF4552
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 186,400 + GFF4549 0.28 -1.6 186,400 + GFF4549 0.28 -2.7 186,401 - GFF4549 0.28 +0.6 186,401 - GFF4549 0.28 -0.2 186,401 - GFF4549 0.28 -0.6 186,401 - GFF4549 0.28 -0.0 186,401 - GFF4549 0.28 +0.1 186,401 - GFF4549 0.28 -1.2 186,463 + GFF4549 0.35 +2.8 187,012 + +0.2 187,012 + -0.5 187,012 + -0.5 187,013 - +0.0 187,013 - +0.5 187,030 + -0.5 187,030 + +0.7 187,030 + -0.4 187,030 + -2.6 187,030 + -0.5 187,031 - +0.3 187,031 - -0.4 187,031 - +0.4 187,031 - -0.1 187,031 - +1.6 187,031 - -0.9 187,031 - +0.1 187,116 + -0.3 187,116 + +0.1 187,116 + +0.2 187,116 + -0.3 187,117 - -0.3 187,117 - -1.1 187,120 + -0.3 187,120 + -1.5 187,120 + -0.9 187,120 + -0.0 187,120 + -0.5 187,120 + +0.7 187,120 + -1.6 187,120 + +0.4 187,120 + -0.4 187,120 + -0.5 187,120 + -1.4 187,120 + -0.5 187,121 - +0.3 187,121 - -0.2 187,121 - +1.3 187,121 - -0.2 187,121 - -0.5 187,121 - -0.5 187,121 - -0.3 187,121 - +0.4 187,121 - +0.6 187,121 - +0.8 187,129 + +0.8 187,129 + -1.4 187,252 + -2.1 187,252 + -0.2 187,252 + -0.7 187,252 + -0.4 187,252 + +0.4 187,252 + -1.5 187,252 + -0.2 187,253 - -0.0 187,253 - -0.3 187,253 - +0.0 187,253 - +0.8 187,253 - -0.5 187,253 - +0.2 187,297 + GFF4550 0.12 -1.2 187,297 + GFF4550 0.12 -0.1 187,297 + GFF4550 0.12 -0.7 187,297 + GFF4550 0.12 -0.2 187,297 + GFF4550 0.12 +0.1 187,297 + GFF4550 0.12 +0.6 187,297 + GFF4550 0.12 +0.3 187,297 + GFF4550 0.12 -1.4 187,298 - GFF4550 0.12 +0.5 187,298 - GFF4550 0.12 -0.2 187,298 - GFF4550 0.12 +2.2 187,298 - GFF4550 0.12 +0.6 187,298 - GFF4550 0.12 +0.7 187,298 - GFF4550 0.12 +0.2 187,298 - GFF4550 0.12 +0.4 187,298 - GFF4550 0.12 -0.2 187,298 - GFF4550 0.12 +0.3 187,298 - GFF4550 0.12 -0.1 187,298 - GFF4550 0.12 +0.8 187,298 - GFF4550 0.12 -0.1 187,298 - GFF4550 0.12 -0.2 187,298 - GFF4550 0.12 -1.2 187,396 + GFF4550 0.18 +0.8 187,396 + GFF4550 0.18 -0.2 187,397 - GFF4550 0.18 -0.8 187,397 - GFF4550 0.18 -1.7 187,645 + GFF4550 0.32 -0.0 187,645 + GFF4550 0.32 -0.0 187,645 + GFF4550 0.32 -0.1 187,645 + GFF4550 0.32 -1.0 187,645 + GFF4550 0.32 +0.5 187,645 + GFF4550 0.32 -0.3 187,646 - GFF4550 0.32 -0.1 187,646 - GFF4550 0.32 -1.7 187,729 + GFF4550 0.37 -1.0 187,729 + GFF4550 0.37 -0.3 187,729 + GFF4550 0.37 +1.7 187,729 + GFF4550 0.37 +0.5 187,730 - GFF4550 0.37 -0.8 187,730 - GFF4550 0.37 -0.4 187,730 - GFF4550 0.37 +0.0 187,848 + GFF4550 0.44 -0.1 188,104 + GFF4550 0.60 -0.2 188,104 + GFF4550 0.60 +0.4 188,104 + GFF4550 0.60 -0.1 188,104 + GFF4550 0.60 -0.2 188,104 + GFF4550 0.60 -0.9 188,105 - GFF4550 0.60 +2.4 188,105 - GFF4550 0.60 -0.9 188,105 - GFF4550 0.60 -0.5 188,105 - GFF4550 0.60 -0.3 188,143 + GFF4550 0.62 +1.1 188,143 + GFF4550 0.62 +0.5 188,143 + GFF4550 0.62 -0.5 188,143 + GFF4550 0.62 +0.0 188,143 + GFF4550 0.62 -1.1 188,144 - GFF4550 0.62 +0.2 188,144 - GFF4550 0.62 -1.2 188,144 - GFF4550 0.62 +0.0 188,144 - GFF4550 0.62 -0.4 188,144 - GFF4550 0.62 -0.5 188,227 + GFF4550 0.67 +0.8 188,227 + GFF4550 0.67 -0.7 188,228 - GFF4550 0.67 -0.4 188,288 - GFF4550 0.71 -0.3 188,365 + GFF4550 0.75 +1.5 188,443 + GFF4550 0.80 -0.1 188,443 + GFF4550 0.80 -0.5 188,443 + GFF4550 0.80 +0.8 188,443 + GFF4550 0.80 +0.5 188,443 + GFF4550 0.80 +0.6 188,444 - GFF4550 0.80 -1.1 188,444 - GFF4550 0.80 -0.0 188,444 - GFF4550 0.80 -0.2 188,444 - GFF4550 0.80 -0.6 188,491 + GFF4550 0.83 +0.7 188,491 + GFF4550 0.83 -1.5 188,491 + GFF4550 0.83 +0.5 188,492 - GFF4550 0.83 -0.7 188,677 + -0.7 188,678 - -0.5 188,928 + GFF4551 0.25 +1.3 188,928 + GFF4551 0.25 +0.0 188,928 + GFF4551 0.25 +0.4 188,929 - GFF4551 0.25 -1.0 188,929 - GFF4551 0.25 -0.7 188,929 - GFF4551 0.25 +0.6 188,929 - GFF4551 0.25 +0.6 188,929 - GFF4551 0.25 +2.1 188,929 - GFF4551 0.25 -0.6 188,940 + GFF4551 0.27 +0.4 188,940 + GFF4551 0.27 +0.6 188,940 + GFF4551 0.27 -1.2 188,940 + GFF4551 0.27 +1.2 188,940 + GFF4551 0.27 +0.5 188,940 + GFF4551 0.27 +1.2 188,941 - GFF4551 0.28 +0.2 188,941 - GFF4551 0.28 -0.3 188,941 - GFF4551 0.28 +0.5 189,392 + -0.0 189,392 + -0.8 189,392 + -0.2 189,392 + -1.2 189,392 + -0.3 189,392 + +0.8 189,392 + -1.0 189,393 - -1.0 189,393 - +0.1 189,393 - -0.0 189,393 - -0.2 189,393 - +0.7 189,393 - -1.9 189,509 + GFF4552 0.23 +0.3 189,533 + GFF4552 0.27 +0.8 189,533 + GFF4552 0.27 -0.3 189,534 - GFF4552 0.27 -1.5 189,534 - GFF4552 0.27 -0.5 189,534 - GFF4552 0.27 -0.3 189,534 - GFF4552 0.27 -0.4 189,534 - GFF4552 0.27 -0.7 189,534 - GFF4552 0.27 -0.4 189,534 - GFF4552 0.27 -0.2 189,534 - GFF4552 0.27 -0.8 189,534 - GFF4552 0.27 -0.8
Or see this region's nucleotide sequence