Strain Fitness in Variovorax sp. SCN45 around GFF448

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF446 and GFF447 overlap by 4 nucleotidesGFF447 and GFF448 overlap by 4 nucleotidesGFF448 and GFF449 overlap by 17 nucleotides GFF446 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 484,125 to 485,150 GFF446 GFF447 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 485,147 to 486,019 GFF447 GFF448 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 486,016 to 486,621 GFF448 GFF449 - Cobyrinic acid a,c-diamide synthetase (EC 6.3.5.11), at 486,605 to 488,026 GFF449 Position (kb) 486 487Strain fitness (log2 ratio) -2 -1 0 1 2at 485.367 kb on + strand, within GFF447at 485.367 kb on + strand, within GFF447at 485.367 kb on + strand, within GFF447at 485.368 kb on - strand, within GFF447at 485.368 kb on - strand, within GFF447at 485.368 kb on - strand, within GFF447at 485.368 kb on - strand, within GFF447at 485.497 kb on - strand, within GFF447at 485.497 kb on - strand, within GFF447at 486.464 kb on + strand, within GFF448at 486.464 kb on + strand, within GFF448at 486.464 kb on + strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.606 kb on + strandat 486.606 kb on + strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.695 kb on + strandat 486.699 kb on + strandat 486.699 kb on + strandat 486.699 kb on + strandat 486.700 kb on - strandat 486.700 kb on - strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.819 kb on + strand, within GFF449at 486.819 kb on + strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 487.210 kb on - strand, within GFF449at 487.585 kb on - strand, within GFF449

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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485,367 + GFF447 0.25 -0.3
485,367 + GFF447 0.25 -0.8
485,367 + GFF447 0.25 +0.6
485,368 - GFF447 0.25 -0.4
485,368 - GFF447 0.25 -0.7
485,368 - GFF447 0.25 +0.8
485,368 - GFF447 0.25 +0.5
485,497 - GFF447 0.40 -2.0
485,497 - GFF447 0.40 -0.5
486,464 + GFF448 0.74 -1.0
486,464 + GFF448 0.74 -1.7
486,464 + GFF448 0.74 -0.1
486,465 - GFF448 0.74 -0.7
486,465 - GFF448 0.74 +0.2
486,465 - GFF448 0.74 -0.0
486,465 - GFF448 0.74 -1.6
486,465 - GFF448 0.74 +1.3
486,606 + +1.9
486,606 + +0.1
486,607 - -0.9
486,607 - -0.7
486,607 - +0.6
486,607 - -1.7
486,607 - +0.7
486,695 + +1.0
486,699 + -0.3
486,699 + -0.6
486,699 + -0.2
486,700 - +1.3
486,700 - -0.8
486,726 + +0.1
486,726 + -0.3
486,726 + -1.3
486,726 + -1.4
486,726 + -0.7
486,726 + -0.2
486,726 + -2.0
486,727 - -0.6
486,727 - -0.1
486,727 - -1.3
486,727 - +1.9
486,727 - -1.0
486,727 - +0.4
486,819 + GFF449 0.15 +0.2
486,819 + GFF449 0.15 +0.2
486,820 - GFF449 0.15 +0.1
486,820 - GFF449 0.15 -0.3
486,820 - GFF449 0.15 +0.9
486,820 - GFF449 0.15 -0.6
487,210 - GFF449 0.43 +2.3
487,585 - GFF449 0.69 -0.2

Or see this region's nucleotide sequence