Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF405 and GFF406 are separated by 27 nucleotides GFF406 and GFF407 are separated by 73 nucleotides GFF407 and GFF408 are separated by 53 nucleotides GFF408 and GFF409 are separated by 74 nucleotides
GFF405 - 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 440,225 to 440,995
GFF405
GFF406 - hypothetical protein, at 441,023 to 441,727
GFF406
GFF407 - no description, at 441,801 to 442,283
GFF407
GFF408 - Two-component system response regulator OmpR, at 442,337 to 443,068
GFF408
GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564
GFF409
Position (kb) 441
442
443 Strain fitness (log2 ratio) -2
-1
0
1
2 at 440.993 kb on + strand at 440.993 kb on + strand at 440.994 kb on - strand at 440.994 kb on - strand at 441.011 kb on + strand at 441.012 kb on - strand at 441.024 kb on + strand at 441.024 kb on + strand at 441.024 kb on + strand at 441.024 kb on - strand at 441.025 kb on - strand at 441.025 kb on - strand at 441.025 kb on - strand at 441.025 kb on - strand at 441.025 kb on - strand at 441.187 kb on - strand, within GFF406 at 441.426 kb on + strand, within GFF406 at 441.426 kb on + strand, within GFF406 at 441.426 kb on + strand, within GFF406 at 441.427 kb on - strand, within GFF406 at 441.427 kb on - strand, within GFF406 at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.732 kb on + strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.733 kb on - strand at 441.750 kb on - strand at 441.750 kb on - strand at 441.750 kb on - strand at 441.750 kb on - strand at 441.750 kb on - strand at 441.952 kb on + strand, within GFF407 at 441.952 kb on + strand, within GFF407 at 441.952 kb on + strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 441.953 kb on - strand, within GFF407 at 442.082 kb on - strand, within GFF407 at 442.177 kb on + strand, within GFF407 at 442.178 kb on - strand, within GFF407 at 442.178 kb on - strand, within GFF407 at 442.248 kb on + strand at 442.253 kb on + strand at 442.253 kb on + strand at 442.254 kb on - strand at 442.254 kb on - strand at 442.266 kb on + strand at 442.266 kb on + strand at 442.266 kb on + strand at 442.267 kb on - strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.316 kb on + strand at 442.332 kb on + strand at 442.333 kb on - strand at 442.416 kb on - strand, within GFF408 at 442.652 kb on + strand, within GFF408 at 442.653 kb on - strand, within GFF408 at 442.653 kb on - strand, within GFF408 at 443.012 kb on + strand at 443.012 kb on + strand at 443.012 kb on + strand at 443.013 kb on - strand at 443.013 kb on - strand at 443.013 kb on - strand at 443.039 kb on + strand at 443.039 kb on + strand at 443.040 kb on - strand at 443.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 440,993 + -0.9 440,993 + +0.6 440,994 - +0.8 440,994 - -0.6 441,011 + -0.5 441,012 - -0.1 441,024 + -1.0 441,024 + -0.5 441,024 + +0.0 441,024 - -2.1 441,025 - -0.2 441,025 - +0.1 441,025 - -0.2 441,025 - -0.5 441,025 - -0.3 441,187 - GFF406 0.23 +1.9 441,426 + GFF406 0.57 +1.6 441,426 + GFF406 0.57 -1.3 441,426 + GFF406 0.57 +1.1 441,427 - GFF406 0.57 -0.8 441,427 - GFF406 0.57 +0.6 441,732 + +0.7 441,732 + +0.1 441,732 + +0.1 441,732 + -0.7 441,732 + +0.1 441,732 + -0.1 441,732 + -0.1 441,732 + -0.7 441,732 + +1.3 441,732 + -0.2 441,732 + -0.2 441,732 + -2.1 441,732 + -0.3 441,732 + -0.7 441,732 + +2.3 441,732 + -0.1 441,732 + +0.2 441,732 + -1.0 441,732 + -2.1 441,733 - +0.3 441,733 - +0.6 441,733 - -0.3 441,733 - -0.3 441,733 - +0.2 441,733 - -0.3 441,733 - -0.4 441,733 - -1.1 441,733 - +0.2 441,733 - +0.2 441,733 - +0.3 441,733 - -0.5 441,733 - +0.1 441,733 - +0.2 441,733 - -0.4 441,733 - -0.0 441,733 - +0.4 441,733 - +0.3 441,733 - +0.5 441,733 - -0.2 441,733 - +1.0 441,733 - -0.6 441,750 - -0.2 441,750 - -0.4 441,750 - +0.5 441,750 - +0.1 441,750 - +1.7 441,952 + GFF407 0.31 -0.2 441,952 + GFF407 0.31 -0.6 441,952 + GFF407 0.31 -1.3 441,953 - GFF407 0.31 -0.6 441,953 - GFF407 0.31 -1.3 441,953 - GFF407 0.31 -0.1 441,953 - GFF407 0.31 +0.8 441,953 - GFF407 0.31 -0.7 441,953 - GFF407 0.31 -1.3 441,953 - GFF407 0.31 +0.4 442,082 - GFF407 0.58 +1.3 442,177 + GFF407 0.78 +0.2 442,178 - GFF407 0.78 -0.0 442,178 - GFF407 0.78 -0.5 442,248 + +0.0 442,253 + -1.0 442,253 + -1.7 442,254 - +0.4 442,254 - -0.1 442,266 + +0.3 442,266 + -0.3 442,266 + +0.6 442,267 - +0.3 442,270 + -0.8 442,270 + +0.4 442,270 + +0.1 442,270 + -2.1 442,270 + -0.2 442,270 + +0.3 442,270 + -0.3 442,270 + -0.7 442,270 + -1.6 442,270 + -0.7 442,271 - -0.3 442,271 - -0.1 442,271 - +0.7 442,271 - -0.0 442,271 - -0.5 442,271 - +0.4 442,271 - -0.3 442,271 - -0.7 442,271 - +1.1 442,316 + -0.2 442,332 + -0.9 442,333 - -1.5 442,416 - GFF408 0.11 -1.0 442,652 + GFF408 0.43 -0.7 442,653 - GFF408 0.43 -2.1 442,653 - GFF408 0.43 -1.0 443,012 + -2.0 443,012 + -1.0 443,012 + +1.3 443,013 - -1.9 443,013 - -0.7 443,013 - +1.3 443,039 + +0.3 443,039 + -2.0 443,040 - -1.0 443,040 - -2.0
Or see this region's nucleotide sequence