Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3963 and GFF3964 overlap by 4 nucleotides GFF3964 and GFF3965 overlap by 4 nucleotides GFF3965 and GFF3966 are separated by 41 nucleotides GFF3966 and GFF3967 are separated by 144 nucleotides
GFF3963 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 164,624 to 165,628
GFF3963
GFF3964 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 165,625 to 166,428
GFF3964
GFF3965 - Alcohol dehydrogenase, zinc-binding, at 166,425 to 167,309
GFF3965
GFF3966 - Homoprotocatechuate degradative operon repressor, at 167,351 to 167,827
GFF3966
GFF3967 - Indolepyruvate oxidoreductase subunit IorA (EC 1.2.7.8), at 167,972 to 170,164
GFF3967
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 165.440 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.513 kb on - strand, within GFF3963 at 165.519 kb on - strand, within GFF3963 at 165.676 kb on + strand at 165.676 kb on + strand at 165.749 kb on - strand, within GFF3964 at 165.749 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.004 kb on - strand, within GFF3964 at 166.594 kb on - strand, within GFF3965 at 166.745 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 167.058 kb on + strand, within GFF3965 at 167.784 kb on + strand at 168.101 kb on + strand at 168.102 kb on - strand at 168.237 kb on - strand, within GFF3967
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 165,440 + GFF3963 0.81 +0.1 165,512 + GFF3963 0.88 -1.6 165,512 + GFF3963 0.88 -1.6 165,513 - GFF3963 0.88 -1.3 165,519 - GFF3963 0.89 +0.1 165,676 + +0.4 165,676 + -0.9 165,749 - GFF3964 0.15 -1.0 165,749 - GFF3964 0.15 +1.2 165,912 - GFF3964 0.36 -1.2 165,912 - GFF3964 0.36 -0.1 165,912 - GFF3964 0.36 +0.3 166,003 + GFF3964 0.47 -1.0 166,003 + GFF3964 0.47 +0.2 166,003 + GFF3964 0.47 +1.3 166,004 - GFF3964 0.47 +0.4 166,594 - GFF3965 0.19 +0.0 166,745 - GFF3965 0.36 +1.2 166,982 - GFF3965 0.63 -0.1 166,982 - GFF3965 0.63 -0.3 166,982 - GFF3965 0.63 +0.2 166,985 + GFF3965 0.63 -0.1 166,985 + GFF3965 0.63 -1.7 166,985 + GFF3965 0.63 -0.2 166,985 + GFF3965 0.63 -0.5 166,985 + GFF3965 0.63 -0.1 166,986 - GFF3965 0.63 +0.1 166,986 - GFF3965 0.63 +0.3 166,986 - GFF3965 0.63 -0.9 166,986 - GFF3965 0.63 -0.9 166,986 - GFF3965 0.63 -0.7 166,986 - GFF3965 0.63 -1.3 166,986 - GFF3965 0.63 -1.5 167,058 + GFF3965 0.72 -0.3 167,784 + +0.5 168,101 + -0.3 168,102 - -0.7 168,237 - GFF3967 0.12 -0.2
Or see this region's nucleotide sequence