Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF36 and GFF37 overlap by 4 nucleotides GFF37 and GFF38 are separated by 12 nucleotides GFF38 and GFF39 are separated by 44 nucleotides GFF39 and GFF40 are separated by 76 nucleotides
GFF36 - Similar to Agmatine deiminase, at 39,121 to 40,245
GFF36
GFF37 - Similar to Agmatine deiminase, at 40,242 to 41,195
GFF37
GFF38 - no description, at 41,208 to 41,465
GFF38
GFF39 - Transcriptional regulator, LysR family, at 41,510 to 42,370
GFF39
GFF40 - no description, at 42,447 to 43,592
GFF40
Position (kb) 41
42 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 40.429 kb on + strand, within GFF37 at 40.429 kb on + strand, within GFF37 at 40.430 kb on - strand, within GFF37 at 40.430 kb on - strand, within GFF37 at 40.430 kb on - strand, within GFF37 at 40.630 kb on + strand, within GFF37 at 40.889 kb on + strand, within GFF37 at 40.890 kb on - strand, within GFF37 at 41.102 kb on - strand at 41.102 kb on - strand at 41.169 kb on + strand at 41.420 kb on + strand, within GFF38 at 41.420 kb on + strand, within GFF38 at 41.420 kb on + strand, within GFF38 at 41.421 kb on - strand, within GFF38 at 41.421 kb on - strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.526 kb on + strand at 41.526 kb on + strand at 41.526 kb on + strand at 41.526 kb on + strand at 41.527 kb on - strand at 41.527 kb on - strand at 41.527 kb on - strand at 41.623 kb on - strand, within GFF39 at 41.739 kb on + strand, within GFF39 at 41.740 kb on - strand, within GFF39 at 41.740 kb on - strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.962 kb on - strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.102 kb on + strand, within GFF39 at 42.297 kb on + strand at 42.297 kb on + strand at 42.297 kb on + strand at 42.298 kb on - strand at 42.298 kb on - strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 40,429 + GFF37 0.20 -2.4 40,429 + GFF37 0.20 +0.4 40,430 - GFF37 0.20 -0.3 40,430 - GFF37 0.20 -0.9 40,430 - GFF37 0.20 -0.7 40,630 + GFF37 0.41 +0.7 40,889 + GFF37 0.68 -0.5 40,890 - GFF37 0.68 -1.0 41,102 - +0.6 41,102 - +0.2 41,169 + +0.3 41,420 + GFF38 0.82 +0.3 41,420 + GFF38 0.82 +0.3 41,420 + GFF38 0.82 +1.1 41,421 - GFF38 0.83 -0.4 41,421 - GFF38 0.83 +3.4 41,423 + GFF38 0.83 -0.6 41,423 + GFF38 0.83 -0.4 41,423 + GFF38 0.83 -0.2 41,424 - GFF38 0.84 -0.4 41,424 - GFF38 0.84 -0.3 41,424 - GFF38 0.84 -0.2 41,424 - GFF38 0.84 +0.9 41,526 + -0.4 41,526 + +0.5 41,526 + -0.3 41,526 + -0.6 41,527 - -0.4 41,527 - +1.6 41,527 - -0.1 41,623 - GFF39 0.13 +0.3 41,739 + GFF39 0.27 -0.9 41,740 - GFF39 0.27 -0.7 41,740 - GFF39 0.27 -0.0 41,961 + GFF39 0.52 +0.2 41,961 + GFF39 0.52 +1.3 41,961 + GFF39 0.52 -0.3 41,962 - GFF39 0.52 +0.1 42,096 + GFF39 0.68 -0.6 42,096 + GFF39 0.68 +0.6 42,096 + GFF39 0.68 -0.2 42,096 + GFF39 0.68 -0.1 42,097 - GFF39 0.68 -0.7 42,097 - GFF39 0.68 -1.8 42,097 - GFF39 0.68 +0.1 42,097 - GFF39 0.68 -1.6 42,102 + GFF39 0.69 +1.0 42,297 + +0.6 42,297 + -0.7 42,297 + -0.7 42,298 - -0.1 42,298 - +0.1 42,408 + -1.2 42,408 + -0.6 42,408 + -0.2 42,408 + +1.3 42,408 + +0.5 42,409 - +1.3 42,409 - -1.9 42,409 - -0.2 42,409 - -0.1 42,409 - -1.5
Or see this region's nucleotide sequence