Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3762 and GFF3763 are separated by 56 nucleotides GFF3763 and GFF3764 are separated by 6 nucleotides GFF3764 and GFF3765 overlap by 4 nucleotides
GFF3762 - hypothetical protein, at 254,406 to 255,440
GFF3762
GFF3763 - no description, at 255,497 to 256,015
GFF3763
GFF3764 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 256,022 to 256,753
GFF3764
GFF3765 - no description, at 256,750 to 257,751
GFF3765
Position (kb) 255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 254.659 kb on - strand, within GFF3762 at 254.659 kb on - strand, within GFF3762 at 254.892 kb on + strand, within GFF3762 at 254.893 kb on - strand, within GFF3762 at 255.108 kb on + strand, within GFF3762 at 255.108 kb on + strand, within GFF3762 at 255.109 kb on - strand, within GFF3762 at 255.109 kb on - strand, within GFF3762 at 255.456 kb on + strand at 255.457 kb on - strand at 255.524 kb on + strand at 255.525 kb on - strand at 255.677 kb on + strand, within GFF3763 at 255.677 kb on + strand, within GFF3763 at 255.678 kb on - strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 256.040 kb on + strand at 256.040 kb on + strand at 256.040 kb on + strand at 256.040 kb on + strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.112 kb on + strand, within GFF3764 at 256.112 kb on + strand, within GFF3764 at 256.112 kb on + strand, within GFF3764 at 256.113 kb on - strand, within GFF3764 at 256.211 kb on + strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.232 kb on + strand, within GFF3764 at 256.232 kb on + strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.777 kb on + strand at 256.777 kb on + strand at 256.864 kb on + strand, within GFF3765 at 256.909 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.970 kb on - strand, within GFF3765 at 256.985 kb on - strand, within GFF3765
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 254,659 - GFF3762 0.24 +0.2 254,659 - GFF3762 0.24 +0.2 254,892 + GFF3762 0.47 -2.0 254,893 - GFF3762 0.47 +0.4 255,108 + GFF3762 0.68 -2.8 255,108 + GFF3762 0.68 -0.0 255,109 - GFF3762 0.68 -0.2 255,109 - GFF3762 0.68 +0.7 255,456 + -0.5 255,457 - +0.5 255,524 + -0.7 255,525 - +0.7 255,677 + GFF3763 0.35 +0.1 255,677 + GFF3763 0.35 +0.2 255,678 - GFF3763 0.35 +0.7 255,752 + GFF3763 0.49 -0.9 255,752 + GFF3763 0.49 -0.2 255,752 + GFF3763 0.49 +0.6 255,752 + GFF3763 0.49 +0.2 255,752 + GFF3763 0.49 -2.5 255,752 + GFF3763 0.49 +0.3 255,752 + GFF3763 0.49 +0.9 255,753 - GFF3763 0.49 -0.3 255,753 - GFF3763 0.49 -0.2 255,753 - GFF3763 0.49 -0.5 255,753 - GFF3763 0.49 -1.2 255,753 - GFF3763 0.49 -0.6 255,753 - GFF3763 0.49 +1.9 255,753 - GFF3763 0.49 +0.6 255,770 + GFF3763 0.53 +1.1 255,770 + GFF3763 0.53 -0.5 255,770 + GFF3763 0.53 -0.0 255,771 - GFF3763 0.53 -0.4 255,771 - GFF3763 0.53 +0.9 255,771 - GFF3763 0.53 -0.9 255,771 - GFF3763 0.53 -0.1 256,040 + -1.0 256,040 + +0.4 256,040 + -1.0 256,040 + -0.1 256,041 - -0.5 256,041 - -0.1 256,041 - -1.0 256,041 - -1.4 256,041 - +0.6 256,112 + GFF3764 0.12 -1.0 256,112 + GFF3764 0.12 -0.1 256,112 + GFF3764 0.12 -0.3 256,113 - GFF3764 0.12 -0.8 256,211 + GFF3764 0.26 -0.4 256,212 - GFF3764 0.26 +1.1 256,212 - GFF3764 0.26 +0.3 256,212 - GFF3764 0.26 +0.3 256,212 - GFF3764 0.26 -0.1 256,212 - GFF3764 0.26 +0.3 256,232 + GFF3764 0.29 -0.1 256,232 + GFF3764 0.29 -1.0 256,233 - GFF3764 0.29 -0.4 256,233 - GFF3764 0.29 -0.0 256,233 - GFF3764 0.29 -1.2 256,233 - GFF3764 0.29 -0.2 256,233 - GFF3764 0.29 -0.1 256,777 + +0.6 256,777 + +1.6 256,864 + GFF3765 0.11 +0.1 256,909 + GFF3765 0.16 -0.4 256,969 + GFF3765 0.22 -0.8 256,969 + GFF3765 0.22 +2.0 256,969 + GFF3765 0.22 +2.3 256,969 + GFF3765 0.22 +1.3 256,969 + GFF3765 0.22 +0.5 256,969 + GFF3765 0.22 -1.1 256,969 + GFF3765 0.22 +0.5 256,970 - GFF3765 0.22 +0.3 256,985 - GFF3765 0.23 -1.5
Or see this region's nucleotide sequence