Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3611 and GFF3612 are separated by 34 nucleotides GFF3612 and GFF3613 are separated by 7 nucleotides GFF3613 and GFF3614 are separated by 8 nucleotides
GFF3611 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 124,379 to 126,235
GFF3611
GFF3612 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 126,270 to 127,436
GFF3612
GFF3613 - Protein of unknown function DUF55, at 127,444 to 127,917
GFF3613
GFF3614 - UPF0056 inner membrane protein YchE, at 127,926 to 128,579
GFF3614
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 125.273 kb on + strand, within GFF3611 at 125.274 kb on - strand, within GFF3611 at 125.327 kb on + strand, within GFF3611 at 125.328 kb on - strand, within GFF3611 at 125.328 kb on - strand, within GFF3611 at 125.328 kb on - strand, within GFF3611 at 125.525 kb on + strand, within GFF3611 at 125.525 kb on + strand, within GFF3611 at 125.526 kb on - strand, within GFF3611 at 125.526 kb on - strand, within GFF3611 at 125.526 kb on - strand, within GFF3611 at 125.643 kb on - strand, within GFF3611 at 125.900 kb on + strand, within GFF3611 at 125.900 kb on + strand, within GFF3611 at 125.900 kb on + strand, within GFF3611 at 125.901 kb on - strand, within GFF3611 at 125.901 kb on - strand, within GFF3611 at 125.939 kb on + strand, within GFF3611 at 125.940 kb on - strand, within GFF3611 at 125.940 kb on - strand, within GFF3611 at 126.017 kb on + strand, within GFF3611 at 126.018 kb on - strand, within GFF3611 at 126.203 kb on + strand at 126.204 kb on - strand at 126.204 kb on - strand at 126.204 kb on - strand at 126.216 kb on + strand at 126.216 kb on + strand at 126.216 kb on + strand at 126.217 kb on - strand at 126.217 kb on - strand at 126.217 kb on - strand at 126.217 kb on - strand at 126.227 kb on + strand at 126.228 kb on - strand at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.648 kb on + strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.649 kb on - strand, within GFF3612 at 126.678 kb on + strand, within GFF3612 at 126.679 kb on - strand, within GFF3612 at 126.679 kb on - strand, within GFF3612 at 126.702 kb on + strand, within GFF3612 at 126.702 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.720 kb on + strand, within GFF3612 at 126.721 kb on - strand, within GFF3612 at 126.721 kb on - strand, within GFF3612 at 126.721 kb on - strand, within GFF3612 at 126.721 kb on - strand, within GFF3612 at 126.721 kb on - strand, within GFF3612 at 126.723 kb on + strand, within GFF3612 at 126.724 kb on - strand, within GFF3612 at 126.724 kb on - strand, within GFF3612 at 126.724 kb on - strand, within GFF3612 at 126.724 kb on - strand, within GFF3612 at 126.792 kb on + strand, within GFF3612 at 126.793 kb on - strand, within GFF3612 at 127.045 kb on - strand, within GFF3612 at 127.045 kb on - strand, within GFF3612 at 127.092 kb on + strand, within GFF3612 at 127.092 kb on + strand, within GFF3612 at 127.092 kb on + strand, within GFF3612 at 127.092 kb on + strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.093 kb on - strand, within GFF3612 at 127.120 kb on - strand, within GFF3612 at 127.120 kb on - strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.164 kb on + strand, within GFF3612 at 127.165 kb on - strand, within GFF3612 at 127.165 kb on - strand, within GFF3612 at 127.165 kb on - strand, within GFF3612 at 127.207 kb on - strand, within GFF3612 at 127.212 kb on + strand, within GFF3612 at 127.213 kb on - strand, within GFF3612 at 127.341 kb on + strand at 127.341 kb on + strand at 127.341 kb on + strand at 127.341 kb on + strand at 127.342 kb on - strand at 127.342 kb on - strand at 127.342 kb on - strand at 127.342 kb on - strand at 127.422 kb on + strand at 127.422 kb on + strand at 127.422 kb on + strand at 127.423 kb on - strand at 127.423 kb on - strand at 127.423 kb on - strand at 127.423 kb on - strand at 127.423 kb on - strand at 127.558 kb on + strand, within GFF3613 at 127.558 kb on + strand, within GFF3613 at 127.558 kb on + strand, within GFF3613 at 127.558 kb on + strand, within GFF3613 at 127.558 kb on + strand, within GFF3613 at 127.559 kb on - strand, within GFF3613 at 127.559 kb on - strand, within GFF3613 at 127.615 kb on + strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.616 kb on - strand, within GFF3613 at 127.711 kb on + strand, within GFF3613 at 127.712 kb on - strand, within GFF3613 at 127.712 kb on - strand, within GFF3613 at 127.714 kb on + strand, within GFF3613 at 127.714 kb on + strand, within GFF3613 at 127.714 kb on + strand, within GFF3613 at 127.715 kb on - strand, within GFF3613 at 127.715 kb on - strand, within GFF3613 at 127.715 kb on - strand, within GFF3613 at 127.945 kb on - strand at 128.114 kb on - strand, within GFF3614 at 128.161 kb on + strand, within GFF3614
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 125,273 + GFF3611 0.48 -1.5 125,274 - GFF3611 0.48 +0.4 125,327 + GFF3611 0.51 -0.9 125,328 - GFF3611 0.51 -1.4 125,328 - GFF3611 0.51 -0.2 125,328 - GFF3611 0.51 +0.0 125,525 + GFF3611 0.62 +0.6 125,525 + GFF3611 0.62 +0.5 125,526 - GFF3611 0.62 -0.5 125,526 - GFF3611 0.62 -0.2 125,526 - GFF3611 0.62 +0.2 125,643 - GFF3611 0.68 -0.5 125,900 + GFF3611 0.82 +0.2 125,900 + GFF3611 0.82 +0.9 125,900 + GFF3611 0.82 -0.4 125,901 - GFF3611 0.82 +1.2 125,901 - GFF3611 0.82 -0.3 125,939 + GFF3611 0.84 -1.8 125,940 - GFF3611 0.84 +2.2 125,940 - GFF3611 0.84 -0.0 126,017 + GFF3611 0.88 +0.3 126,018 - GFF3611 0.88 +0.4 126,203 + +0.1 126,204 - +0.4 126,204 - +0.6 126,204 - -0.4 126,216 + -1.3 126,216 + +0.7 126,216 + -0.7 126,217 - -1.5 126,217 - -0.2 126,217 - +0.1 126,217 - -0.4 126,227 + -0.1 126,228 - +0.2 126,648 + GFF3612 0.32 -0.5 126,648 + GFF3612 0.32 -0.3 126,648 + GFF3612 0.32 +1.1 126,648 + GFF3612 0.32 -0.6 126,648 + GFF3612 0.32 +0.9 126,648 + GFF3612 0.32 -0.2 126,648 + GFF3612 0.32 +0.1 126,649 - GFF3612 0.32 -0.4 126,649 - GFF3612 0.32 -0.5 126,649 - GFF3612 0.32 +0.3 126,649 - GFF3612 0.32 +0.5 126,649 - GFF3612 0.32 -0.2 126,649 - GFF3612 0.32 -0.3 126,649 - GFF3612 0.32 -0.3 126,678 + GFF3612 0.35 -0.1 126,679 - GFF3612 0.35 -2.0 126,679 - GFF3612 0.35 +0.5 126,702 + GFF3612 0.37 +0.0 126,702 + GFF3612 0.37 +0.9 126,720 + GFF3612 0.39 +1.2 126,720 + GFF3612 0.39 -0.2 126,720 + GFF3612 0.39 -0.0 126,720 + GFF3612 0.39 +0.0 126,720 + GFF3612 0.39 +0.3 126,720 + GFF3612 0.39 -0.4 126,721 - GFF3612 0.39 -0.9 126,721 - GFF3612 0.39 -0.1 126,721 - GFF3612 0.39 +0.0 126,721 - GFF3612 0.39 +0.4 126,721 - GFF3612 0.39 +1.1 126,723 + GFF3612 0.39 +0.3 126,724 - GFF3612 0.39 +0.3 126,724 - GFF3612 0.39 +1.1 126,724 - GFF3612 0.39 -0.4 126,724 - GFF3612 0.39 +1.2 126,792 + GFF3612 0.45 +0.3 126,793 - GFF3612 0.45 +0.6 127,045 - GFF3612 0.66 +0.4 127,045 - GFF3612 0.66 -0.4 127,092 + GFF3612 0.70 -0.8 127,092 + GFF3612 0.70 -0.1 127,092 + GFF3612 0.70 +0.1 127,092 + GFF3612 0.70 +0.5 127,093 - GFF3612 0.71 -0.5 127,093 - GFF3612 0.71 +1.3 127,093 - GFF3612 0.71 +0.5 127,093 - GFF3612 0.71 +0.5 127,093 - GFF3612 0.71 +0.2 127,093 - GFF3612 0.71 +0.2 127,093 - GFF3612 0.71 +0.2 127,120 - GFF3612 0.73 +0.1 127,120 - GFF3612 0.73 -0.4 127,164 + GFF3612 0.77 -0.3 127,164 + GFF3612 0.77 +0.9 127,164 + GFF3612 0.77 -0.4 127,164 + GFF3612 0.77 -0.3 127,164 + GFF3612 0.77 +0.7 127,164 + GFF3612 0.77 +0.5 127,164 + GFF3612 0.77 +0.7 127,165 - GFF3612 0.77 +0.2 127,165 - GFF3612 0.77 -0.5 127,165 - GFF3612 0.77 +0.1 127,207 - GFF3612 0.80 -0.1 127,212 + GFF3612 0.81 +0.2 127,213 - GFF3612 0.81 -1.2 127,341 + +0.8 127,341 + +1.4 127,341 + +0.3 127,341 + +0.7 127,342 - -0.2 127,342 - -0.2 127,342 - -0.5 127,342 - -0.5 127,422 + +0.2 127,422 + +0.1 127,422 + +0.2 127,423 - +0.0 127,423 - -0.6 127,423 - +0.0 127,423 - +0.1 127,423 - +0.7 127,558 + GFF3613 0.24 -0.9 127,558 + GFF3613 0.24 +1.6 127,558 + GFF3613 0.24 +0.1 127,558 + GFF3613 0.24 -2.2 127,558 + GFF3613 0.24 +0.1 127,559 - GFF3613 0.24 -0.5 127,559 - GFF3613 0.24 +2.5 127,615 + GFF3613 0.36 +0.2 127,616 - GFF3613 0.36 +0.1 127,616 - GFF3613 0.36 +1.4 127,616 - GFF3613 0.36 -0.3 127,616 - GFF3613 0.36 -1.7 127,616 - GFF3613 0.36 +0.9 127,616 - GFF3613 0.36 +1.2 127,711 + GFF3613 0.56 -0.8 127,712 - GFF3613 0.57 -1.1 127,712 - GFF3613 0.57 +0.1 127,714 + GFF3613 0.57 -0.4 127,714 + GFF3613 0.57 +0.8 127,714 + GFF3613 0.57 +0.2 127,715 - GFF3613 0.57 +0.3 127,715 - GFF3613 0.57 +0.6 127,715 - GFF3613 0.57 -0.2 127,945 - -2.2 128,114 - GFF3614 0.29 +1.3 128,161 + GFF3614 0.36 -0.2
Or see this region's nucleotide sequence