Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3598 and GFF3599 are separated by 177 nucleotides GFF3599 and GFF3600 are separated by 90 nucleotides GFF3600 and GFF3601 are separated by 20 nucleotides
GFF3598 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 111,538 to 112,653
GFF3598
GFF3599 - Rhodanese-related sulfurtransferase, at 112,831 to 113,241
GFF3599
GFF3600 - Pantothenate kinase type III, CoaX-like (EC 2.7.1.33), at 113,332 to 114,111
GFF3600
GFF3601 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily, at 114,132 to 115,472
GFF3601
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.346 kb on + strand, within GFF3598 at 112.347 kb on - strand, within GFF3598 at 112.490 kb on + strand, within GFF3598 at 112.491 kb on - strand, within GFF3598 at 112.491 kb on - strand, within GFF3598 at 112.503 kb on - strand, within GFF3598 at 112.583 kb on + strand at 112.711 kb on + strand at 112.711 kb on + strand at 112.712 kb on - strand at 112.712 kb on - strand at 112.712 kb on - strand at 112.712 kb on - strand at 112.858 kb on + strand at 112.858 kb on + strand at 112.859 kb on - strand at 112.859 kb on - strand at 112.859 kb on - strand at 112.960 kb on + strand, within GFF3599 at 112.961 kb on - strand, within GFF3599 at 112.961 kb on - strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.140 kb on + strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.141 kb on - strand, within GFF3599 at 113.322 kb on - strand at 113.368 kb on - strand at 113.390 kb on - strand at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.926 kb on + strand, within GFF3600 at 113.927 kb on - strand, within GFF3600 at 113.927 kb on - strand, within GFF3600 at 113.998 kb on + strand, within GFF3600 at 113.998 kb on + strand, within GFF3600 at 113.998 kb on + strand, within GFF3600 at 113.999 kb on - strand, within GFF3600 at 114.112 kb on + strand at 114.113 kb on - strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.259 kb on + strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.260 kb on - strand at 114.292 kb on + strand, within GFF3601 at 114.292 kb on + strand, within GFF3601 at 114.292 kb on + strand, within GFF3601 at 114.292 kb on + strand, within GFF3601 at 114.292 kb on + strand, within GFF3601 at 114.292 kb on + strand, within GFF3601 at 114.293 kb on - strand, within GFF3601 at 114.293 kb on - strand, within GFF3601 at 114.293 kb on - strand, within GFF3601 at 114.293 kb on - strand, within GFF3601 at 114.667 kb on + strand, within GFF3601 at 114.667 kb on + strand, within GFF3601 at 114.668 kb on - strand, within GFF3601 at 114.668 kb on - strand, within GFF3601 at 114.668 kb on - strand, within GFF3601 at 114.668 kb on - strand, within GFF3601 at 114.721 kb on + strand, within GFF3601 at 114.721 kb on + strand, within GFF3601 at 114.722 kb on - strand, within GFF3601 at 114.742 kb on + strand, within GFF3601 at 114.781 kb on + strand, within GFF3601 at 114.781 kb on + strand, within GFF3601 at 114.781 kb on + strand, within GFF3601 at 114.782 kb on - strand, within GFF3601 at 114.811 kb on + strand, within GFF3601 at 114.811 kb on + strand, within GFF3601 at 114.811 kb on + strand, within GFF3601 at 114.811 kb on + strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.812 kb on - strand, within GFF3601 at 114.823 kb on + strand, within GFF3601 at 114.823 kb on + strand, within GFF3601 at 114.823 kb on + strand, within GFF3601 at 114.824 kb on - strand, within GFF3601 at 114.824 kb on - strand, within GFF3601 at 114.824 kb on - strand, within GFF3601 at 114.874 kb on + strand, within GFF3601 at 114.875 kb on - strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.901 kb on + strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 114.902 kb on - strand, within GFF3601 at 115.027 kb on + strand, within GFF3601 at 115.027 kb on + strand, within GFF3601 at 115.028 kb on - strand, within GFF3601 at 115.028 kb on - strand, within GFF3601 at 115.078 kb on + strand, within GFF3601 at 115.078 kb on + strand, within GFF3601 at 115.078 kb on + strand, within GFF3601 at 115.078 kb on + strand, within GFF3601 at 115.078 kb on + strand, within GFF3601 at 115.079 kb on - strand, within GFF3601 at 115.079 kb on - strand, within GFF3601 at 115.079 kb on - strand, within GFF3601
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 112,346 + GFF3598 0.72 +0.2 112,347 - GFF3598 0.72 +0.3 112,490 + GFF3598 0.85 -2.2 112,491 - GFF3598 0.85 -0.3 112,491 - GFF3598 0.85 -0.4 112,503 - GFF3598 0.86 -0.4 112,583 + +0.4 112,711 + -0.6 112,711 + -0.3 112,712 - -0.1 112,712 - +0.1 112,712 - -0.5 112,712 - +0.6 112,858 + -0.1 112,858 + -0.4 112,859 - -0.7 112,859 - -0.1 112,859 - +0.1 112,960 + GFF3599 0.31 -1.0 112,961 - GFF3599 0.32 -0.1 112,961 - GFF3599 0.32 +0.7 113,140 + GFF3599 0.75 +0.4 113,140 + GFF3599 0.75 +0.2 113,140 + GFF3599 0.75 -0.5 113,140 + GFF3599 0.75 +0.4 113,140 + GFF3599 0.75 -0.1 113,140 + GFF3599 0.75 -0.0 113,140 + GFF3599 0.75 +0.2 113,140 + GFF3599 0.75 -0.1 113,140 + GFF3599 0.75 +0.5 113,140 + GFF3599 0.75 +1.3 113,140 + GFF3599 0.75 -0.7 113,140 + GFF3599 0.75 +0.4 113,140 + GFF3599 0.75 +2.1 113,140 + GFF3599 0.75 -0.1 113,140 + GFF3599 0.75 +1.4 113,140 + GFF3599 0.75 +0.6 113,141 - GFF3599 0.75 +0.8 113,141 - GFF3599 0.75 -0.4 113,141 - GFF3599 0.75 +0.8 113,141 - GFF3599 0.75 -0.2 113,141 - GFF3599 0.75 +1.0 113,141 - GFF3599 0.75 +1.5 113,141 - GFF3599 0.75 -0.6 113,141 - GFF3599 0.75 +3.1 113,141 - GFF3599 0.75 -0.0 113,141 - GFF3599 0.75 -0.4 113,141 - GFF3599 0.75 -0.3 113,141 - GFF3599 0.75 -0.2 113,141 - GFF3599 0.75 +0.3 113,141 - GFF3599 0.75 -0.3 113,141 - GFF3599 0.75 +0.9 113,322 - -0.7 113,368 - -0.9 113,390 - +0.3 113,926 + GFF3600 0.76 +0.3 113,926 + GFF3600 0.76 -1.1 113,926 + GFF3600 0.76 -1.5 113,926 + GFF3600 0.76 -1.2 113,926 + GFF3600 0.76 -2.4 113,926 + GFF3600 0.76 -0.1 113,926 + GFF3600 0.76 +0.9 113,927 - GFF3600 0.76 +0.9 113,927 - GFF3600 0.76 -1.5 113,998 + GFF3600 0.85 -2.0 113,998 + GFF3600 0.85 -0.1 113,998 + GFF3600 0.85 -0.1 113,999 - GFF3600 0.86 -0.1 114,112 + +0.1 114,113 - -0.1 114,259 + -0.1 114,259 + +1.0 114,259 + +0.9 114,259 + -1.9 114,259 + -0.0 114,259 + -0.5 114,259 + +1.6 114,259 + -0.2 114,260 - -0.1 114,260 - +1.2 114,260 - -0.6 114,260 - -0.7 114,260 - -1.1 114,260 - -0.6 114,260 - -1.5 114,260 - -0.3 114,260 - -0.6 114,260 - -0.2 114,292 + GFF3601 0.12 -1.9 114,292 + GFF3601 0.12 +0.3 114,292 + GFF3601 0.12 -0.5 114,292 + GFF3601 0.12 +0.0 114,292 + GFF3601 0.12 -0.4 114,292 + GFF3601 0.12 +0.4 114,293 - GFF3601 0.12 -1.7 114,293 - GFF3601 0.12 +1.1 114,293 - GFF3601 0.12 +0.5 114,293 - GFF3601 0.12 -0.7 114,667 + GFF3601 0.40 +2.3 114,667 + GFF3601 0.40 -0.7 114,668 - GFF3601 0.40 +1.9 114,668 - GFF3601 0.40 +0.1 114,668 - GFF3601 0.40 +0.0 114,668 - GFF3601 0.40 +0.6 114,721 + GFF3601 0.44 +0.1 114,721 + GFF3601 0.44 -1.7 114,722 - GFF3601 0.44 -1.3 114,742 + GFF3601 0.45 +0.4 114,781 + GFF3601 0.48 +0.8 114,781 + GFF3601 0.48 -0.0 114,781 + GFF3601 0.48 -0.6 114,782 - GFF3601 0.48 -0.5 114,811 + GFF3601 0.51 +0.3 114,811 + GFF3601 0.51 +0.3 114,811 + GFF3601 0.51 -1.2 114,811 + GFF3601 0.51 +0.6 114,812 - GFF3601 0.51 -0.5 114,812 - GFF3601 0.51 +1.7 114,812 - GFF3601 0.51 +0.6 114,812 - GFF3601 0.51 +0.9 114,812 - GFF3601 0.51 -0.4 114,812 - GFF3601 0.51 -0.6 114,823 + GFF3601 0.52 +0.0 114,823 + GFF3601 0.52 -0.1 114,823 + GFF3601 0.52 +0.1 114,824 - GFF3601 0.52 -0.5 114,824 - GFF3601 0.52 +0.4 114,824 - GFF3601 0.52 -0.3 114,874 + GFF3601 0.55 -0.7 114,875 - GFF3601 0.55 +0.0 114,901 + GFF3601 0.57 +0.3 114,901 + GFF3601 0.57 +0.3 114,901 + GFF3601 0.57 +0.1 114,901 + GFF3601 0.57 +0.7 114,901 + GFF3601 0.57 -0.1 114,901 + GFF3601 0.57 -0.1 114,901 + GFF3601 0.57 -1.3 114,901 + GFF3601 0.57 +0.0 114,901 + GFF3601 0.57 -0.9 114,901 + GFF3601 0.57 +0.4 114,902 - GFF3601 0.57 +0.6 114,902 - GFF3601 0.57 -0.7 114,902 - GFF3601 0.57 +0.3 114,902 - GFF3601 0.57 -0.3 114,902 - GFF3601 0.57 -1.2 114,902 - GFF3601 0.57 -0.3 114,902 - GFF3601 0.57 -0.5 114,902 - GFF3601 0.57 -0.5 114,902 - GFF3601 0.57 -0.8 114,902 - GFF3601 0.57 -0.4 114,902 - GFF3601 0.57 -1.3 114,902 - GFF3601 0.57 -0.8 114,902 - GFF3601 0.57 +1.1 115,027 + GFF3601 0.67 -1.0 115,027 + GFF3601 0.67 -0.1 115,028 - GFF3601 0.67 -0.2 115,028 - GFF3601 0.67 -0.8 115,078 + GFF3601 0.71 -0.7 115,078 + GFF3601 0.71 -1.6 115,078 + GFF3601 0.71 -0.3 115,078 + GFF3601 0.71 -0.4 115,078 + GFF3601 0.71 +0.0 115,079 - GFF3601 0.71 -0.7 115,079 - GFF3601 0.71 -0.1 115,079 - GFF3601 0.71 -1.1
Or see this region's nucleotide sequence